Abstract: The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.

Abstract: Provided herein are methods, devices, kits, and articles of manufacture for detecting pregnancy-associated plasma protein-A (PAPP-A) in a biological sample, for instance a whole blood or serum sample obtained from a pregnant subject, and estimating gestational age (GA) based on the detection of PAPP-A, such that clinical or personal decisions informed by GA can be made without ultrasound.

Abstract: Bone fusion system, devices, guides, implant and methods for using the bone fusion system, devices, guides and implant are disclosed. The fusion system includes a first alignment guide, a second alignment guide, and an implant. The first alignment guide couples to an intermediate portion of the implant and the second alignment guide couples to a distal portion of the implant. The implant includes a body portion, a first extension portion extending away from a first end of the body portion, a second extension portion extending away from a posterior of the body portion, and a third extension portion extending away from a distal end of the body portion. Finally, methods for using the bone fusion system, devices, guide and implant are disclosed.

Abstract: Data comprising a request specifying a desired change in electrical load for a defined period of time is received. The electrical load is associated with a plurality of resources under control of resource agents and on a utility grid. Available capacity of resources within the control of each resource agent over the defined period of time is determined. Each available capacity is determined at least using a resource load model. Using the determined available capacity of resources over the defined period of time, resource scheduling instructions are calculated for a subset of resources. The resource scheduling instructions satisfy the desired change in electrical load of the request. Data characterizing the resource scheduling instructions is provided. Related systems, apparatus, methods, and articles are also described.

Abstract: Data comprising a request specifying a desired change in electrical load for a defined period of time is received. The electrical load is associated with a plurality of resources under control of resource agents and on a utility grid. Available capacity of resources within the control of each resource agent over the defined period of time is determined. Each available capacity is determined at least using a resource load model. Using the determined available capacity of resources over the defined period of time, resource scheduling instructions are calculated for a subset of resources. The resource scheduling instructions satisfy the desired change in electrical load of the request. Data characterizing the resource scheduling instructions is provided. Related systems, apparatus, methods, and articles are also described.