Patents by Inventor Lauren Sears Higgins

Lauren Sears Higgins has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • N. bstnbi nicking endonuclease and methods for using endonucleases in single-stranded displacement amplification
    Publication number: 20030211506
    Abstract: The present invention relates to recombinant DNA which encodes a novel nicking endonuclease, N.BstNBI, and the production of N.BstNBI restriction endonuclease from the recombinant DNA utilizing PleI modification methylase. Related expression vectors, as well as the application of N.BstNBI and other nicking enzymes in non-modified strand displacement amplification, is disclosed also.
    Type: Application
    Filed: November 14, 2002
    Publication date: November 13, 2003
    Inventors: Huimin Kong, Lauren Sears Higgins, Michael A Dalton, Rebecca B Kucera, Ira Schildkraut, Geoffrey G Wilson
  • Method for cloning and expression of MlyI restriction endonuclease and MlyI methylase and BstNBII methylase in E. coli
    Patent number: 6395531
    Abstract: The present invention relates to recombinant DNA which encodes the MlyI restriction endonuclease as well as the MlyI and BstNBII methyltransferases and expression of MlyI restriction endonuclease and M.BStNBII in E. coli cells containing the recombinant DNA.
    Type: Grant
    Filed: March 21, 2001
    Date of Patent: May 28, 2002
    Assignee: New England Biolabs, Inc.
    Inventors: Huimin Kong, Lauren Sears Higgins
  • Method for cloning and expression of PleI restriction endonuclease and PleI and BstNBII methylases in E. coli
    Patent number: 6391608
    Abstract: The present invention relates to recombinant DNA which encodes the PleI restriction endonuclease as well as PleI and BstNBII methyltransferase and expression of PleI restriction endonuclease and M.BstNBII in E. coli cells containing the recombinant DNA.
    Type: Grant
    Filed: March 21, 2001
    Date of Patent: May 21, 2002
    Assignee: New England Biolabs, Inc.
    Inventors: Huimin Kong, Lauren Sears Higgins