Abstract: A process is provided for the production of lymphocytosis promoting factor (LPF), filamentous haemagglutinin (FHA) and at least one fimbrial agglutinogen from a liquid culture of Bordetella pertussis, which comprises the steps of (a) separating the culture into cellular and supernatant fractions, (b) concentrating the supernatant fraction, (c) fractionating the concentrated supernatant fraction to isolate LPF and FHA containing fractions, and (d) isolating at least one fimbrial agglutinogen from the cellular fraction. A vaccine composition may be produced by mixing so-produced LPF, FHA and fimbrial agglutinogens produced.

Abstract: The Leukocytosis Promoting Factor (LPF) of Bordetella Haemagglutinin (HG) is separated from crude cell supernatant or partially purified protein by affinity chromatography on a column material consisting of an insoluble polymeric support to which is bound a sialoprotein (glycoprotein containing sialic acid) or other substance rich in sialic acid.The sialoprotein was preferably a plasma sialoprotein, such as haptoglobin or ceruloplasmin, or a salivery mucin, and the polymeric support was preferably an agarose gel, though other conventional supports could be used.By this process, on treatment of an ammonium sulphate precipitated extract, the haemagglinating activity may be increased 300-600 fold over the extract and 10,000 times over the crude centrifuged cell supernatant. Alternatively a fraction substantially free from LPF-HG may be collected.