Patents by Inventor Laurene Cheung

Laurene Cheung has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods of producing aggregate-free monomeric diphtheria toxin fusion proteins and therapeutic uses
    Patent number: 11965009
    Abstract: The present invention is a DNA expression vector comprising: a toxP: a mutant toxO that blocks Fe-mediated regulation of gene expression; and a DNA sequence encoding a protein, wherein the toxP and the mutant toxO regulate expression of the DNA segment encoding the protein. It is preferred that DNA expression vectors of the present invention include DNA sequences encoding a signal peptide so that a protein expressed is attached to the signal peptide prior to processing. Novel proteins are produced off of the DNA expression vector of the present invention.
    Type: Grant
    Filed: December 13, 2021
    Date of Patent: April 23, 2024
    Assignees: The Johns Hopkins University, Trustees of Boston University
    Inventors: William R. Bishai, John R. Murphy, Laurene Cheung, Shashank Gupta, Cynthia K. Bullen
  • METHODS OF PRODUCING AGGREGATE-FREE MONOMERIC DIPHTHERIA TOXIN FUSION PROTEINS AND THERAPEUTIC USES
    Publication number: 20230183307
    Abstract: The present invention is a DNA expression vector comprising: a toxP: a mutant toxO that blocks Fe-mediated regulation of gene expression; and a DNA sequence encoding a protein, wherein the toxP and the mutant toxO regulate expression of the DNA segment encoding the protein. It is preferred that DNA expression vectors of the present invention include DNA sequences encoding a signal peptide so that a protein expressed is attached to the signal peptide prior to processing. Novel proteins are produced off of the DNA expression vector of the present invention.
    Type: Application
    Filed: December 13, 2021
    Publication date: June 15, 2023
    Inventors: William R. Bishai, John R. Murphy, Laurene Cheung, Shashank Gupta, Cynthia K. Bullen
  • Methods of treating cancer using bacteria expressing c-di-AMP
    Patent number: 11590177
    Abstract: The present invention includes the discovery of a strain of Mycobacterium comprising an expression vector encoding a di-adenylate cyclase enzyme. The Mycobacterium is selected from the group consisting of Mycobacterium tuberculosis, Mycobacterium bovis, or a combination thereof and the preferred strain of Mycobacterium is BCG. The preferred expression vector is a mycobacterial expression vector including an hsp60 promoter and a DNA sequence of diadenylate cyclase (disA), or a functional part thereof. The strains of Mycobacterium are used in therapeutic applications including tuberculosis and cancer.
    Type: Grant
    Filed: November 19, 2020
    Date of Patent: February 28, 2023
    Assignee: The Johns Hopkins University
    Inventors: William R. Bishai, Ruchi Jain Dey, Bappaditya Dey, Laurene Cheung
  • METHODS OF PRODUCING AGGREGATE-FREE MONOMERIC DIPHTHERIA TOXIN FUSION PROTEINS AND THERAPEUTIC USES
    Publication number: 20220204579
    Abstract: The present invention is a DNA expression vector comprising: a toxP: a mutant toxO that blocks Fe-mediated regulation of gene expression; and a DNA sequence encoding a protein, wherein the toxP and the mutant toxO regulate expression of the DNA segment encoding the protein. It is preferred that DNA expression vectors of the present invention include DNA sequences encoding a signal peptide so that a protein expressed is attached to the signal peptide prior to processing. Novel proteins are produced off of the DNA expression vector of the present invention.
    Type: Application
    Filed: December 13, 2021
    Publication date: June 30, 2022
    Inventors: William R. Bishai, John R. Murphy, Laurene Cheung, Shashank Gupta, Cynthia K. Bullen
  • Methods of producing aggregate-free monomeric diphtheria toxin fusion proteins and therapeutic uses
    Patent number: 11203626
    Abstract: The present invention is a DNA expression vector comprising: a toxP; a mutant toxO that blocks Fe-mediated regulation of gene expression; and a DNA sequence encoding a protein, wherein the toxP and the mutant toxO regulate expression of the DNA segment encoding the protein. It is preferred that DNA expression vectors of the present invention include DNA sequences encoding a signal peptide so that a protein expressed is attached to the signal peptide prior to processing. Novel proteins are produced off of the DNA expression vector of the present invention.
    Type: Grant
    Filed: September 11, 2017
    Date of Patent: December 21, 2021
    Assignees: The Johns Hopkins University, Trustees of Boston University
    Inventors: William R. Bishai, John R. Murphy, Laurene Cheung, Shashank Gupta, Cynthia K. Bullen
  • Methods of Treating Cancer Using Bacteria Expressing c-di-AMP
    Publication number: 20210145896
    Abstract: The present invention includes the discovery of a strain of Mycobacterium comprising an expression vector encoding a di-adenylate cyclase enzyme. The Mycobacterium is selected from the group consisting of Mycobacterium tuberculosis, Mycobacterium bovis, or a combination thereof and the preferred strain of Mycobacterium is BCG. The preferred expression vector is a mycobacterial expression vector including an hsp60 promoter and a DNA sequence of diadenylate cyclase (disA), or a functional part thereof. The strains of Mycobacterium are used in therapeutic applications including tuberculosis and cancer.
    Type: Application
    Filed: November 19, 2020
    Publication date: May 20, 2021
    Inventors: William R. Bishai, Ruchi Jain Dey, Bappaditya Dey, Laurene Cheung
  • Methods of producing aggregate-free monomeric diphtheria toxin fusion proteins and therapeutic uses
    Patent number: 10988512
    Abstract: The present invention is a DNA expression vector comprising: a toxP; a mutant toxO that blocks Fe-mediated regulation of gene expression; and a DNA sequence encoding a protein, wherein the toxP and the mutant toxO regulate expression of the DNA segment encoding the protein. It is preferred that DNA expression vectors of the present invention include DNA sequences encoding a signal peptide so that a protein expressed is attached to the signal peptide prior to processing. Novel proteins are produced off of the DNA expression vector of the present invention.
    Type: Grant
    Filed: March 10, 2017
    Date of Patent: April 27, 2021
    Assignees: The Johns Hopkins University, Trustees of Boston University
    Inventors: William R. Bishai, John R. Murphy, Laurene Cheung, Shashank Gupta, Cynthia K. Bullen
  • Methods of treating cancer using bacteria expressing c-di-AMP
    Patent number: 10842828
    Abstract: Described herein are methods of treating or preventing cancer in a subject comprising administering to the subject an effective amount of a bacteria comprising and expression vector encoding a di-adenylate cyclase enzyme.
    Type: Grant
    Filed: October 1, 2018
    Date of Patent: November 24, 2020
    Assignee: The Johns Hopkins University
    Inventors: William R. Bishai, Ruchi Jain Dey, Bappaditya Dey, Laurene Cheung
  • METHODS OF PRODUCING AGGREGATE-FREE MONOMERIC DIPHTHERIA TOXIN FUSION PROTEINS AND THERAPEUTIC USES
    Publication number: 20200157166
    Abstract: The present invention is a DNA expression vector comprising: a toxP; a mutant toxO that blocks Fe-mediated regulation of gene expression; and a DNA sequence encoding a protein, wherein the toxP and the mutant toxO regulate expression of the DNA segment encoding the protein. It is preferred that DNA expression vectors of the present invention include DNA sequences encoding a signal peptide so that a protein expressed is attached to the signal peptide prior to processing. Novel proteins are produced off of the DNA expression vector of the present invention.
    Type: Application
    Filed: September 11, 2017
    Publication date: May 21, 2020
    Inventors: William R. Bishai, John R. Murphy, Laurene Cheung, Shashank Gupta, Cynthia K. Bullen
  • Methods of Producing Aggregate-Free Monomeric Diphtheria Toxin Fusion Proteins and Therapeutic Uses
    Publication number: 20190071472
    Abstract: The present invention is a DNA expression vector comprising: a toxP; a mutant toxO that blocks Fe-mediated regulation of gene expression; and a DNA sequence encoding a protein, wherein the toxP and the mutant toxO regulate expression of the DNA segment encoding the protein. It is preferred that DNA expression vectors of the present invention include DNA sequences encoding a signal peptide so that a protein expressed is attached to the signal peptide prior to processing. Novel proteins are produced off of the DNA expression vector of the present invention.
    Type: Application
    Filed: March 10, 2017
    Publication date: March 7, 2019
    Applicants: The Johns Hopkins University, Trustees of Boston University
    Inventors: William R. BISHAI, John R. MURPHY, Laurene CHEUNG, Shashank GUPTA, Cynthia K. BULLEN
  • Methods of treating cancer using bacteria expressing c-di-AMP
    Publication number: 20190030091
    Abstract: Described herein are methods of treating or preventing cancer in a subject comprising administering to the subject an effective amount of a bacteria comprising and expression vector encoding a di-adenylate cyclase enzyme.
    Type: Application
    Filed: October 1, 2018
    Publication date: January 31, 2019
    Inventors: William R. Bishai, Ruchi Jain Dey, Bappaditya Dey, Laurene Cheung
  • Bacteria over-expressing c-di-AMP and therapeutic methods
    Patent number: 10130663
    Abstract: The present invention includes the discovery of a strain of Mycobacterium comprising an expression vector encoding a di-adenylate cyclase enzyme. The Mycobacterium is selected from the group consisting of Mycobacterium tuberculosis, Mycobacterium bovis, or a combination thereof and the preferred strain of Mycobacterium is BCG. The preferred expression vector is a mycobacterial expression vector including an hsp60 promoter and a DNA sequence of diadenylate cyclase (disA), or a functional part thereof. The strains of Mycobacterium are used in therapeutic applications including tuberculosis and cancer.
    Type: Grant
    Filed: February 10, 2016
    Date of Patent: November 20, 2018
    Assignee: The John Hopkins University
    Inventors: William R. Bishai, Ruchi Jain Dey, Bappaditya Dey, Laurene Cheung
  • BACTERIA OVER-EXPRESSING C-DI-AMP AND THERAPEUTIC METHODS
    Publication number: 20180028577
    Abstract: The present invention includes the discovery of a strain of Mycobacterium comprising an expression vector encoding a di-adenylate cyclase enzyme. The Mycobacterium is selected from the group consisting of Mycobacterium tuberculosis, Mycobacterium bovis, or a combination thereof and the preferred strain of Mycobacterium is BCG. The preferred expression vector is a mycobacterial expression vector including an hsp60 promoter and a DNA sequence of diadenylate cyclase (disA), or a functional part thereof. The strains of Mycobacterium are used in therapeutic applications including tuberculosis and cancer.
    Type: Application
    Filed: February 10, 2016
    Publication date: February 1, 2018
    Applicant: The John Hopkins University
    Inventors: William R. Bishai, Ruchi Jain Dey, Bappaditya Dey, Laurene Cheung