Patents by Inventor Laurent Fourage
Laurent Fourage has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230220431Abstract: There is provided SHC/HAC derivatives, amino acid sequences comprising the SHC/HAC derivatives, nucleotide sequences encoding the SHC/HAC derivatives, vectors comprising nucleotide sequences encoding the SHC/HAC derivatives, recombinant host cells comprising nucleotide sequences encoding the SHC/HAC derivatives and applications of the recombinant host cells comprising either SHC/HAC derivatives or WT SHC/HAC enzymes in methods to prepare (?)-Ambrox and SHC/HAC enzymes in methods to prepare (?)-Ambrox.Type: ApplicationFiled: August 31, 2022Publication date: July 13, 2023Inventors: Eric EICHHORN, Boris SCHILLING, Denis WAHLER, Laurent FOURAGE, Esther LOCHER
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Patent number: 11466299Abstract: There is provided SHC/HAC derivatives, amino acid sequences comprising the SHC/HAC derivatives, nucleotide sequences encoding the SHC/HAC derivatives, vectors comprising nucleotide sequences encoding the SHC/HAC derivatives, recombinant host cells comprising nucleotide sequences encoding the SHC/HAC derivatives and applications of the recombinant host cells comprising either SHC/HAC derivatives or WT SHC/HAC enzymes in methods to prepare (?)-Ambrox and SHC/HAC enzymes in methods to prepare (?)-Ambrox.Type: GrantFiled: April 26, 2021Date of Patent: October 11, 2022Assignee: GIVAUDAN S.A.Inventors: Eric Eichhorn, Boris Schilling, Denis Wahler, Laurent Fourage, Esther Locher
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Publication number: 20220145313Abstract: The application generally relates to chloroplast targeting of nuclear-encoded proteins of interest in microalgae. Provided herein are expression cassettes comprising a nucleotide sequence encoding a chloroplast targeting peptide operably linked to a nucleotide sequence encoding a protein of interest, wherein said chloroplast targeting peptide comprises the bipartite targeting sequence of the phosphoribulokinase of Nannochloropsis gaditana (NgPRK BTS). The invention further provides vectors comprising the expression cassettes, and microalgae having stably incorporated or transiently expressed into their nuclear genomes an expression cassette described above. Methods are also provided for the production of a protein of interest in the chloroplast of a microalga, as well as methods for modulating chloroplast pathways.Type: ApplicationFiled: September 23, 2021Publication date: May 12, 2022Inventors: Leonardo MAGNECHI, Eric MARECHAL, Giovanni FINAZZI, Mariette BEDHOMME, Séverine COLLIN, Frédéric LAEUFFER, Laurent FOURAGE
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Patent number: 11162107Abstract: The application generally relates to chloroplast targeting of nuclear-encoded proteins of interest in microalgae. Provided herein are expression cassettes comprising a nucleotide sequence encoding a chloroplast targeting peptide operably linked to a nucleotide sequence encoding a protein of interest, wherein said chloroplast targeting peptide comprises the bipartite targeting sequence of the phosphoribulokinase of Nannochloropsis gaditana (NgPRK BTS). The invention further provides vectors comprising the expression cassettes, and microalgae having stably incorporated or transiently expressed into their nuclear genomes an expression cassette described above. Methods are also provided for the production of a protein of interest in the chloroplast of a microalga, as well as methods for modulating chloroplast pathways.Type: GrantFiled: December 5, 2017Date of Patent: November 2, 2021Assignee: Total Raffinage ChimieInventors: Leonardo Magneschi, Eric Marechal, Giovanni Finazzi, Mariette Bedhomme, Séverine Collin, Frédéric Laeuffer, Laurent Fourage
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Publication number: 20210292733Abstract: The present invention relates to biocatalysts catalyzing the formation of ?-olefins. In particular, the invention provides polypeptides with improved decarboxylase activity on C10-C16 free fatty acids, more particularly on C10 or C12 free fatty acids, as compared to the P450 fatty acid decarboxylase isolated from the Staphylococcus massiliensis strain S46 (Sm46). The invention further provides recombinant nucleic acids and vectors comprising the coding sequences encoding these polypeptides, genetically engineered host cells expressing said polypeptides and methods for the production of C9-C15 ?-olefins, more particularly C9 or C11 ?-olefins, using said polypeptides or said host cells.Type: ApplicationFiled: July 25, 2019Publication date: September 23, 2021Applicants: TOTAL RAFFINAGE CHIMIE, QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECHNOLOGY (QIBEBT)Inventors: Shengying LI, Huifang XU, Linlin NING, Laurent FOURAGE
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Publication number: 20210254112Abstract: There is provided SHC/HAC derivatives, amino acid sequences comprising the SHC/HAC derivatives, nucleotide sequences encoding the SHC/HAC derivatives, vectors comprising nucleotide sequences encoding the SHC/HAC derivatives, recombinant host cells comprising nucleotide sequences encoding the SHC/HAC derivatives and applications of the recombinant host cells comprising either SHC/HAC derivatives or WT SHC/HAC enzymes in methods to prepare (?)-Ambrox and SHC/HAC enzymes in methods to prepare (?)-Ambrox.Type: ApplicationFiled: April 26, 2021Publication date: August 19, 2021Inventors: Eric EICHHORN, Boris SCHILLING, Denis WAHLER, Laurent FOURAGE, Esther LOCHER
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Patent number: 11021722Abstract: There is provided SHC/HAC derivatives, amino acid sequences comprising the SHC/HAC derivatives, nucleotide sequences encoding the SHC/HAC derivatives, vectors comprising nucleotide sequences encoding the SHC/HAC derivatives, recombinant host cells comprising nucleotide sequences encoding the SHC/HAC derivatives and applications of the recombinant host cells comprising either SHC/HAC derivatives or WT SHC/HAC enzymes in methods to prepare (?)-Ambrox and SHC/HAC enzymes in methods to prepare (?)-Ambrox.Type: GrantFiled: October 10, 2019Date of Patent: June 1, 2021Assignee: GIVAUDAN S.A.Inventors: Eric Eichhorn, Boris Schilling, Denis Wahler, Laurent Fourage, Esther Locher
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Publication number: 20200040369Abstract: There is provided SHC/HAC derivatives, amino acid sequences comprising the SHC/HAC derivatives, nucleotide sequences encoding the SHC/HAC derivatives, vectors comprising nucleotide sequences encoding the SHC/HAC derivatives, recombinant host cells comprising nucleotide sequences encoding the SHC/HAC derivatives and applications of the recombinant host cells comprising either SHC/HAC derivatives or WT SHC/HAC enzymes in methods to prepare (?)-Ambrox and SHC/HAC enzymes in methods to prepare (?)-Ambrox.Type: ApplicationFiled: October 10, 2019Publication date: February 6, 2020Inventors: Eric EICHHORN, Boris SCHILLING, Denis WAHLER, Laurent FOURAGE, Esther LOCHER
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Publication number: 20190352346Abstract: The application generally relates to chloroplast targeting of nuclear-encoded proteins of interest in microalgae. Provided herein are expression cassettes comprising a nucleotide sequence encoding a chloroplast targeting peptide operably linked to a nucleotide sequence encoding a protein of interest, wherein said chloroplast targeting peptide comprises the bipartite targeting sequence of the phosphoribulokinase of Nannochloropsis gaditana (NgPRK BTS). The invention further provides vectors comprising the expression cassettes, and microalgae having stably incorporated or transiently expressed into their nuclear genomes an expression cassette described above. Methods are also provided for the production of a protein of interest in the chloroplast of a microalga, as well as methods for modulating chloroplast pathways.Type: ApplicationFiled: December 5, 2017Publication date: November 21, 2019Inventors: Leonardo Magneschi, Eric Marechal, Giovanni Finazzi, Mariette Bedhomme, Séverine Collin, Frédéric Laeuffer, Laurent Fourage
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Patent number: 10472655Abstract: There is provided SHC/HAC derivatives, amino acid sequences comprising the SHC/HAC derivatives, nucleotide sequences encoding the SHC/HAC derivatives, vectors comprising nucleotide sequences encoding the SHC/HAC derivatives, recombinant host cells comprising nucleotide sequences encoding the SHC/HAC derivatives and applications of the recombinant host cells comprising either SHC/HAC derivatives or WT SHC/HAC enzymes in methods to prepare (?)-Ambrox and SHC/HAC enzymes in methods to prepare (?)-Ambrox.Type: GrantFiled: April 22, 2016Date of Patent: November 12, 2019Assignee: GIVAUDAN S.A.Inventors: Eric Eichhorn, Boris Schilling, Denis Wahler, Laurent Fourage, Esther Locher
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Patent number: 10407638Abstract: The present invention relates to the biosynthesis of ?-olefins. In particular, the invention provides methods for the production of medium-chain ?-olefins, more particularly C11 ?-olefins, using a polypeptide with decarboxylase activity on free fatty acids with 8 to 14 carbons, in particular on C8-C12 free fatty acids, more particularly on C12 free fatty acids, or a genetically engineered host cell expressing or overexpressing said polypeptide.Type: GrantFiled: June 30, 2016Date of Patent: September 10, 2019Assignees: Total Raffinage Chimie, Qingdao Institute of Bioenergy and Bioprocess TechnologyInventors: Laurent Fourage, Frédéric Laeuffer, Henri Strub, Yun Wang, Jian Xu, Huifang Xu, Shengying Li
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Publication number: 20190024101Abstract: The present application relates to an expression system for multigene overexpression in microalgae, which expression system comprises at least two nucleic acid expression cassettes, wherein each expression cassette comprises a promoter operably linked to three or more transgenes connected to one another by at least one sequence encoding a 2A peptide (i.e. a multicistronic construct). Also disclosed herein are vector systems comprising said expression systems, host cells transformed with said expression systems or comprising said vector systems, methods for producing these host cells, as well as their use for biosynthesis.Type: ApplicationFiled: January 27, 2017Publication date: January 24, 2019Inventors: Séverine COLLIN, Laurent FOURAGE, Fédéric LAEUFFER, Mariette BEDHOMME, Leonardo MAGNESCHI, Giovanni FINAZZI, Eric MARECHAL
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Publication number: 20180171252Abstract: The present invention relates to the biosynthesis of ?-olefins. In particular, the invention provides methods for the production of medium-chain ?-olefins, more particularly C11 ?-olefins, using a polypeptide with decarboxylase activity on free fatty acids with 8 to 14 carbons, in particular on C8-C12 free fatty acids, more particularly on C12 free fatty acids, or a genetically engineered host cell expressing or overexpressing said polypeptide.Type: ApplicationFiled: June 30, 2016Publication date: June 21, 2018Applicants: TOTAL RAFFINAGE CHIMIE, QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECHNOLOGY (QIBEBT)Inventors: Laurent FOURAGE, Frédéric LAEUFFER, Henri STRUB, Yun WANG, Jian XU, Huifang HU, Shengying LI
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Publication number: 20180148751Abstract: There is provided SHC/HAC derivatives, amino acid sequences comprising the SHC/HAC derivatives, nucleotide sequences encoding the SHC/HAC derivatives, vectors comprising nucleotide sequences encoding the SHC/HAC derivatives, recombinant host cells comprising nucleotide sequences encoding the SHC/HAC derivatives and applications of the recombinant host cells comprising either SHC/HAC derivatives or WT SHC/HAC enzymes in methods to prepare (?)-Ambrox and SHC/HAC enzymes in methods to prepare (?)-Ambrox.Type: ApplicationFiled: April 22, 2016Publication date: May 31, 2018Inventors: Eric EICHHORN, Boris SCHILLING, Denis WAHLER, Laurent FOURAGE, Esther LOCHER
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Patent number: 9309538Abstract: The present invention relates to the expression and optimization of enzymes involved in lignocellulosic biomass decomposition. The present invention relates more particularly to beta-glucosidase variants comprising at least one modification among the amino acids located at positions 225, 238, 240 and 241, according to the numbering in SEQ ID No. 2 of Trichoderma reesei beta-glucosidase, and also relates to the use of said variants having improved effectiveness in methods for cellulose decomposition and biofuel production.Type: GrantFiled: September 10, 2009Date of Patent: April 12, 2016Assignees: IFP Energies Nouvelles, PROTEUSInventors: Nicolas Lopes-Ferreira, Antoine Margeot, Hugues Mathis, Laurent Fourage
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Patent number: 9145570Abstract: The invention relates to an enzymatic acylation method including at least the following steps of: contacting at least one compound having at least one function selected from among the amine, alcohol, or thiol functions, at least one microorganism having an acyl transfer activity and/or an acyl transfer enzyme, and at least one acylphosphonate donor of formula (I), where: R is an alkyl, alkene, alkyne, aryl, or aralkyl radical, or is —ORa, —SRa, —NRaRb, where Ra and Rb are identical or different and are H, an alkyl, alkene, alkyne, aryl or aralkyl radical, the alkyl, alkene, alkyne, aryl or aralkyl radicals being optionally substituted; X is O or S; Y and Z, which are identical or different, are —OR1, —OR2, —SR1, —SR2, —NR?1R?1, —NR?2R?2; R1, R2, R?1, R?2, R?1 and R?2, which are identical or different, are an alkyl, alkene, alkyne, aryl or aralkyl radical, said alkyl, alkene, alkyne, aryl or aralkyl radicals being optionally substituted; and recovering the compound including at least one acyl function, said fuType: GrantFiled: July 29, 2011Date of Patent: September 29, 2015Assignee: PCAS BiosolutionInventors: Juliette Martin, Gerard Guillamot, Laurent Fourage, Denis Wahler
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Patent number: 8951771Abstract: Fatty acid 13-hydroperoxide lyase proteins which have been modified with respect to a previously described guava 13-hydroperoxide lyase and the nucleic acid sequences encoding these proteins. Also, recombinant nucleic acid molecules for expressing the modified 13-hydroperoxide lyases and methods of using such lyases in the field of organic synthesis.Type: GrantFiled: July 26, 2013Date of Patent: February 10, 2015Assignee: Firmenich SAInventors: Fredi Bruhlmann, Laurent Fourage, Denis Wahler
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Publication number: 20140024092Abstract: Fatty acid 13-hydroperoxide lyase proteins which have been modified with respect to a previously described guava 13-hydroperoxide lyase and the nucleic acid sequences encoding these proteins. Also, recombinant nucleic acid molecules for expressing the modified 13-hydroperoxide lyases and methods of using such lyases in the field of organic synthesis.Type: ApplicationFiled: July 26, 2013Publication date: January 23, 2014Inventors: Fredi Bruhlmann, Laurent Fourage, Denis Wahler
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Patent number: 8501452Abstract: Fatty acid 13-hydroperoxide lyase proteins which have been modified with respect to a previously described guava 13-hydroperoxide lyase and the nucleic acid sequences encoding these proteins. Also, recombinant nucleic acid molecules for expressing the modified 13-hydroperoxide lyases and methods of using such lyases in the field of organic synthesis.Type: GrantFiled: June 25, 2008Date of Patent: August 6, 2013Assignee: Firmenich, SAInventors: Fredi Brühlmann, Laurent Fourage, Denis Wahler
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Publication number: 20130130337Abstract: The invention relates to an enzymatic acylation method including at least the following steps of: contacting at least one compound having at least one function selected from among the amine, alcohol, or thiol functions, at least one microorganism having an acyl transfer activity and/or an acyl transfer enzyme, and at least one acylphosphonate donor of formula (I), where: R is an alkyl, alkene, uikyne, aryl, or aralkyl radical, or is —ORa, —SRa, —NRaRb, where Ra and Rb are identical or different and are H, an alkyl, alkene, alkyne, aryl or aralkyl radical, the alkyl, alkene, alkyne, aryl or aralkyl radicals being optionally substituted; X is O or S; Y and Z, which are identical or different, are —OR1, —OR2, —SR1, —SR2, —NR?1R?1, —NR?2R?2; R1, R2, R?1, R?2, R?1 and R?2, which are identical or different, are an alkyl, alkene, alkyne, aryl or aralkyl radical, said alkyl, alkene, alkyne, aryl or aralkyl radicals being optionally substituted; and recovering the compound including at least one acyl function, said fuType: ApplicationFiled: July 29, 2011Publication date: May 23, 2013Applicant: PCAS BIOSOLUTIONInventors: Juliette Martin, Gerard Guillamot, Laurent Fourage, Denis Wahler