Patents by Inventor Laurent Segueilha

Laurent Segueilha has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11473050
    Abstract: The invention relates to a method for the protein enrichment of a heterotrophically cultured microalga, the microalga being of the genus Chlorella, even more particularly Chlorella protothecoides, characterized in that it comprises: a first step directed toward limiting the ammonium supply so as to obtain a microalgal biomass with a protein content of less than 50% expressed as N.6.25, preferably less than 30%, more preferentially between 20 and 25%; a second step in which the ammonium supply in the fermentation medium is increased so as to obtain a protein content of greater than 50%, preferably greater than 60%, more preferentially greater than 65%.
    Type: Grant
    Filed: February 8, 2016
    Date of Patent: October 18, 2022
    Assignee: Corbion Biotech, Inc.
    Inventors: Marie Le Ruyet, Laurent Segueilha, Mélanie Cappe, Sylvain Delaroche
  • Patent number: 11193105
    Abstract: The invention relates to a method for protein enrichment of a microalga grown under heterotrophic conditions, said microalga being of the Chlorella genus, characterized in that the heterotrophic culture comprises a step aimed at limiting the growth of said microalga by means of a deficiency of the fermentation medium in terms of a non-nitrogenous nutritional source.
    Type: Grant
    Filed: January 22, 2019
    Date of Patent: December 7, 2021
    Assignee: Corbion Biotech, Inc.
    Inventors: Gabriel Macquart, Sylvain Delaroche, Marie Le Ruyet, Laurent Segueilha
  • Publication number: 20200046002
    Abstract: The present invention relates to a method for preparing, by fermentation, lipid-rich compositions of microalga flour having optimised organoleptic properties, characterised in that the microalgae are cultured in a minimal culture medium.
    Type: Application
    Filed: August 14, 2019
    Publication date: February 13, 2020
    Applicant: Corbion Biotech, Inc.
    Inventors: Amandine Druon, Marie Le Ruyet, Laurent Segueilha
  • Publication number: 20190345435
    Abstract: The invention relates to a method for the protein enrichment of a heterotrophically cultured microalga, the microalga being of the genus Chlorella, even more particularly Chlorella protothecoides, characterized in that it comprises: a first step directed toward limiting the ammonium supply so as to obtain a microalgal biomass with a protein content of less than 50% expressed as N.6.25, preferably less than 30%, more preferentially between 20 and 25%; a second step in which the ammonium supply in the fermentation medium is increased so as to obtain a protein content of greater than 50%, preferably greater than 60%, more preferentially greater than 65%.
    Type: Application
    Filed: February 8, 2016
    Publication date: November 14, 2019
    Inventors: Marie Le Ruyet, Laurent Segueilha, Mélanie Cappe, Sylvain Delaroche
  • Publication number: 20190218503
    Abstract: The invention relates to a method for protein enrichment of a microalga grown under heterotrophic conditions, said microalga being of the Chlorella genus, characterized in that the heterotrophic culture comprises a step aimed at limiting the growth of said microalga by means of a deficiency of the fermentation medium in terms of a non-nitrogenous nutritional source.
    Type: Application
    Filed: January 22, 2019
    Publication date: July 18, 2019
    Inventors: Gabriel Macquart, Sylvain Delaroche, Marie Le Ruyet, Laurent Segueilha
  • Patent number: 10351814
    Abstract: The invention relates to a method for fermentative production, on an industrial scale, of lipid-rich biomass of microalgae of the Chlorella genus having acceptable sensory properties, characterized in that the dissolved oxygen availability in the fermenter is controlled by tracking the respiratory quotient of said microalgae.
    Type: Grant
    Filed: August 22, 2014
    Date of Patent: July 16, 2019
    Assignee: CORBION BIOTECH, INC.
    Inventors: Sylvain Delaroche, Marie Le Ruyet, Laurent Segueilha, Heike Jerosch, Amandine Druon
  • Patent number: 10280439
    Abstract: The present invention relates to a method for a dicarboxylic acid, which method comprises fermenting fungal cells in a vessel comprising a suitable fermentation medium, wherein a least a portion of the fungal cells are reused in the presence of a vitamin and/or a trace element.
    Type: Grant
    Filed: July 18, 2014
    Date of Patent: May 7, 2019
    Assignees: DSM IP ASSESTS B.V., ROQUETTE FRERES SA
    Inventors: Mickel Leonardus August Jansen, René Verwaal, Laurent Segueilha, Mélanie Louchart, Tania Veiga Dos Inocentes
  • Publication number: 20180312798
    Abstract: The invention related to a method for producing microalgae of the chlorella genus by fed-batch fermentation, characterized in that the provision of a carbon source is carried out sequentially and automatically in response to a drop in oxygen consumption by the microalga, in particular when the dissolved oxygen pressue in the fermentation medium (pO2) exceeds a predefined threshold value.
    Type: Application
    Filed: April 26, 2018
    Publication date: November 1, 2018
    Inventors: Laurent Segueilha, Marie Le Ruyet, Sylvain Delaroche
  • Patent number: 10087467
    Abstract: The invention relates to a method for the production of squalene from microalgae belonging to the family of Thraustochytriales sp., preferably at concentrations of between 2 and 12 g per 100 g of dry biomass. The method is characterized in that it comprises steps consisting in: culturing microalgae belonging to the family of Thraustochytriales sp. at a temperature of between 25 and 35° C., preferably between 28 and 32° C., and more preferably of the order of 30° C.; and adding between 1 and 1000 ?g of vitamin B12 per liter of culture medium to said culture medium.
    Type: Grant
    Filed: May 18, 2012
    Date of Patent: October 2, 2018
    Assignee: Roquette Frares
    Inventors: Bernard Pora, Yun Qian, Bernard Caulier, Serge Comini, Philippe Looten, Laurent Segueilha
  • Publication number: 20180230421
    Abstract: The invention relates to a method for the protein enrichment of a microalga of the genus Chlorella, cultivated under heterotrophic conditions. The method is characterized in that the heterotrophic cultivation comprises a step intended to slow down the growth of the microalga, with the fermentation medium being deficient in a nitrogen-free nutritional source.
    Type: Application
    Filed: April 13, 2018
    Publication date: August 16, 2018
    Inventors: Gabriel Macquart, Sylvain Delaroche, Marie Le Ruyet, Laurent Segueilha
  • Publication number: 20180139993
    Abstract: The invention relates to a method for bleaching a biomass of Chlorella protothecoides microalgae that is rich in protein, said biomass being produced by fermentation, characterised in that it comprises: choosing to produce the microalga biomass under heterotrophic conditions and under continuous fermentation; and varying the colouring of said biomass by monitoring the ratio of the ?/?max growth rates.
    Type: Application
    Filed: May 18, 2016
    Publication date: May 24, 2018
    Applicant: Roquette Freres
    Inventors: Marie Le Ruyet, Laurent Segueilha, Sylvain Delaroche
  • Publication number: 20160376544
    Abstract: The invention relates to a process for the enrichment, with carotenoids and proteins, of a biomass of a microalga cultivated under heterotrophic conditions, wherein said microalga is of the Chlorella genus, which comprises culturing said microalga in a minimum medium supplemented with a nitrogen source in organic form, preferably chosen from the group consisting of yeast extract, corn steep liquor, and a combination thereof.
    Type: Application
    Filed: November 28, 2014
    Publication date: December 29, 2016
    Inventors: MATHIEU COSSART, SOPHIE DEFRETIN, GABRIEL MACQUART, LAURENT SEGUEILHA
  • Publication number: 20160340640
    Abstract: The invention relates to a method for the protein enrichment of a microalga of the genus Chlorella, cultivated under heterotrophic conditions. The method is characterized in that the heterotrophic cultivation comprises a step intended to slow down the growth of the microalga, with the fermentation medium being deficient in a nitrogen-free nutritional source.
    Type: Application
    Filed: January 19, 2015
    Publication date: November 24, 2016
    Inventors: GABRIEL MACQUART, SYLVAIN DELAROCHE, MARIE LE RUYET, LAURENT SEGUEILHA
  • Publication number: 20160326483
    Abstract: The invention relates to a method for producing microalgae of the Chlorella genus by fed-batch fermentation, characterised in that the provision of a carbon source is carried out sequentially and automatically in response to a drop in oxygen consumption by the microalga, in particular when the dissolved oxygen pressure in the fermentation medium (pO2) exceeds a predefined threshold value.
    Type: Application
    Filed: July 25, 2014
    Publication date: November 10, 2016
    Inventors: LAURENT SEGUEILHA, MARIE LE RUYET, SYLVAIN DELAROCHE
  • Publication number: 20160208212
    Abstract: The invention relates to a method for fermentative production, on an industrial scale, of lipid-rich biomass of microalgae of the Chlorella genus having acceptable sensory properties, characterised in that the dissolved oxygen availability in the fermenter is controlled by tracking the respiratory quotient of said microalgae.
    Type: Application
    Filed: August 22, 2014
    Publication date: July 21, 2016
    Inventors: SYLVAIN DELAROCHE, MARIE LE RUYET, LAURENT SEGUEILHA, HEIKE JEROSCH, AMANDINE DRUON
  • Publication number: 20160194670
    Abstract: The present invention relates to a method for a dicarboxylic acid, which method comprises fermenting fungal cells in a vessel comprising a suitable fermentation medium, wherein a least a portion of the fungal cells are reused in the presence of a vitamin and/or a trace element.
    Type: Application
    Filed: July 18, 2014
    Publication date: July 7, 2016
    Inventors: Mickel Leonardus August JANSEN, René VERWAAL, Laurent SEGUEILHA, Mélanie LOUCHART, Tania VEIGA DOS INOCENTES
  • Publication number: 20160192691
    Abstract: The present invention relates to a method for preparing, by fermentation, lipid-rich compositions of microalga flour having optimised organoleptic properties, characterised in that the microalgae are cultured in a minimal culture medium.
    Type: Application
    Filed: August 12, 2014
    Publication date: July 7, 2016
    Inventors: AMANDINE DRUON, MARIE LE RUYET, LAURENT SEGUEILHA
  • Publication number: 20160046900
    Abstract: The invention relates to a method for protein enrichment of a microalga grown under heterotrophic conditions, said microalga being of the Chlorella genus, characterized in that the heterotrophic culture comprises a step aimed at limiting the growth of said microalga by means of a deficiency of the fermentation medium in terms of a non-nitrogenous nutritional source.
    Type: Application
    Filed: March 27, 2014
    Publication date: February 18, 2016
    Inventors: GABRIEL MACQUART, SYLVAIN DELAROCHE, MARIE LE RUYET, LAURENT SEGUEILHA
  • Publication number: 20140088201
    Abstract: The invention relates to a method for the production of squalene from microalgae belonging to the family of Thraustochytriales sp., preferably at concentrations of between 2 and 12 g per 100 g of dry biomass. The method is characterised in that it comprises steps consisting in: culturing microalgae belonging to the family of Thraustochytriales sp. at a temperature of between 25 and 35° C., preferably between 28 and 32° C., and more preferably of the order of 30° C.; and adding between 1 and 1000 ?g of vitamin B12 per litre of culture medium to said culture medium.
    Type: Application
    Filed: May 18, 2012
    Publication date: March 27, 2014
    Applicant: ROQUETTE FRERES
    Inventors: Bernard Pora, Yun Qian, Bernard Caulier, Serge Comini, Philippe Looten, Laurent Segueilha
  • Patent number: 8486686
    Abstract: A new culture method for producing high levels of a metabolite, such as succinic acid uses oxygen rich culture without pH adjustment to increase the biomass, acclimation in under oxygen lean conditions having <5% partial pressure of oxygen, and the production of high levels of succinate under oxygen deprived conditions. The method can be performed in a single reactor, and is amenable to efficient scale up.
    Type: Grant
    Filed: December 28, 2007
    Date of Patent: July 16, 2013
    Assignee: William Marsh Rice University
    Inventors: Laurent Segueilha, Ka-Yiu San, George Bennett, Irene Martinez