Patents by Inventor Lawrence A. Wade
Lawrence A. Wade has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20150322395Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation apparatus described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation apparatus can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation apparatus can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation apparatus can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.Type: ApplicationFiled: July 17, 2015Publication date: November 12, 2015Inventors: Emil P. KARTALOV, Darryl SHIBATA, Clive TAYLOR, Lawrence A. WADE
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Publication number: 20150038379Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation apparatus described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation apparatus can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation apparatus can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation apparatus can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.Type: ApplicationFiled: October 10, 2014Publication date: February 5, 2015Inventors: Emil P. KARTALOV, Darryl SHIBATA, Clive TAYLOR, Lawrence A. WADE
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Patent number: 8889416Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation apparatus described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation apparatus can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation apparatus can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation apparatus can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.Type: GrantFiled: January 20, 2011Date of Patent: November 18, 2014Assignees: California Institute of Technology, University of Southern CaliforniaInventors: Emil P. Kartalov, Darryl Shibata, Clive Taylor, Lawrence A. Wade
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Patent number: 8492160Abstract: The invention is a device including array of aciive regions for use in reacting one or more species in at least two of the active regions in a sequential process, e.g., sequential reactions. The device has a transparent substrate member, which has a surface region and a silane material overlying the surface region. A first active region overlies a first portion of the silane material. The first region has a first dimension of less than 1 micron in size and has first molecules capable of binding to the first portion of the silane material. A second active region overlies a second portion of the silane material. The second region has a second dimension of less than 1 micron in size, second molecules capable of binding to the second portion of the active region, and a spatial distance separates the first active region and the second active region.Type: GrantFiled: March 9, 2010Date of Patent: July 23, 2013Assignee: The United States of America as Represented by the Administrator of the National Aeronautics and Space AdministrationInventors: Lawrence A. Wade, Charles Patrick Collier
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Publication number: 20110177518Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation apparatus described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation apparatus can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation apparatus can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation apparatus can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.Type: ApplicationFiled: January 20, 2011Publication date: July 21, 2011Inventors: Emil P. Kartalov, Darryl Shibata, Clive Taylor, Lawrence A. Wade
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Patent number: 7808628Abstract: Embodiments in accordance with the present invention relate to methods and apparatuses for aligning a scanning probe used to pattern a substrate, by comparing the position of the probe to a reference location or spot on the substrate. A first light beam is focused on a surface of the substrate as a spatial reference point. A second light beam then illuminates the scanning probe being used for patterning. An optical microscope images both the focused light beam, and a diffraction pattern, shadow, or light backscattered by the illuminated scanning probe tip of a scanning probe microscope (SPM), which is typically the tip of the scanning probe on an atomic force microscope (AFM). Alignment of the scanning probe tip relative to the mark is then determined by visual observation of the microscope image. This alignment process may be repeated to allow for modification or changing of the scanning probe microscope tip.Type: GrantFiled: June 21, 2006Date of Patent: October 5, 2010Assignee: California Institute of TechnologyInventor: Lawrence A. Wade
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Patent number: 7514214Abstract: Embodiments in accordance with the present invention relate to techniques for the growth and attachment of single wall carbon nanotubes (SWNT), facilitating their use as robust and well-characterized tools for AFM imaging and other applications. In accordance with one embodiment, SWNTs attached to an AFM tip can function as a structural scaffold for nanoscale device fabrication on a scanning probe. Such a probe can trigger, with nanometer precision, specific biochemical reactions or conformational changes in biological systems. The consequences of such triggering can be observed in real time by single-molecule fluorescence, electrical, and/or AFM sensing. Specific embodiments in accordance with the present invention utilize sensing and manipulation of individual molecules with carbon nanotubes, coupled with single-molecule fluorescence imaging, to allow observation of spectroscopic signals in response to mechanically induced molecular changes.Type: GrantFiled: February 20, 2004Date of Patent: April 7, 2009Assignee: California Institute of TechnologyInventors: Lawrence A. Wade, Ian R. Shapiro, Charles Patrick Collier, Maria J. Esplandiu, Vern Garrett Bittner, Jr., Konstantinos P. Giapis
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Patent number: 7314514Abstract: The invention relates to energy storage devices such as capacitors and supercapacitors and non-aqueous solvent systems suitable for use as an electrolyte solvent therein. Devices incorporating the solvent system are suitable for use in, for example, wireless devices or automotive applications at high temperatures with minimal, if any mass loss. The solvent system has at least one low boiling component (preferably a nitrile, eg acetonitrile) at least one high boiling component compatible with said low boiling component (preferably lactones, eg ?-butyrolactone and/or organic carbonates eg ethylene carbonate or propylene carbonate); and wherein the components are selected in an amount such that said non-aqueous solvent system does not boil at the boiling point of the low viscosity solvent alone but has a boiling point greater than said low viscosity solvent alone.Type: GrantFiled: March 19, 2003Date of Patent: January 1, 2008Assignee: Cap-XX LimitedInventors: Calum John Drummond, Hung Chi Nguyen, Timothy Lawrence Wade
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Patent number: 7247842Abstract: Methods and systems for operating an apertureless microscope for observing one or more features to a molecular sensitivity on objects are described. More particularly, the method includes moving a tip of a probe coupled to a cantilever in a vicinity of a feature of a sample, which emits one or more photons at a detected rate relative to a background rate of the sample based upon the presence of the tip of the probe in the vicinity of the feature. The method modifies the detected rate of the feature of the sample, whereupon the modifying of the detected rate causes the feature of the sample to enhance relative to background rate of the feature.Type: GrantFiled: July 12, 2005Date of Patent: July 24, 2007Assignee: California Institute of TechnologyInventors: Stephen R. Quake, Guillaume Lessard, Lawrence A. Wade, Jordan M. Gerton
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Publication number: 20070152144Abstract: Methods and systems for operating an apertureless microscope for observing one or more features to a molecular sensitivity on objects are described. More particularly, the method includes moving a tip of a probe coupled to a cantilever in a vicinity of a feature of a sample, which emits one or more photons at a detected rate relative to a background rate of the sample based upon the presence of the tip of the probe in the vicinity of the feature. The method modifies the detected rate of the feature of the sample, whereupon the modifying of the detected rate causes the feature of the sample to enhance relative to background rate of the feature.Type: ApplicationFiled: July 12, 2005Publication date: July 5, 2007Applicant: California Institute of TechnologyInventors: Stephen Quake, Guillaume Lessard, Lawrence Wade, Jordan Gerton
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Patent number: 7211795Abstract: A method for fabricating assembled structures. The method includes providing a tip structure, which has a first end, a second end, and a length defined between the first end and the second end. The second end is a free end. The method includes attaching a nano-sized structure along a portion of the length of the tip structure to extend a total length of the tip structure to include the length of the tip structure and a first length associated with the nano-sized structure. The method includes shortening the nano-sized structure from the first length to a second length. The method also includes pushing the nano-sized structure in a direction parallel to the second length to reduce the second length to a third length of the nano-sized structure along the direction parallel to the second length to cause the nano-sized structure to move along a portion of the length of the tip structure.Type: GrantFiled: January 27, 2005Date of Patent: May 1, 2007Assignee: California Institute of TechnologyInventors: Charles Patrick Collier, Ma Ziyang, Stephen R. Quake, Ian R. Shapiro, Lawrence Wade
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Publication number: 20060246467Abstract: A device comprising an array of active regions for use in reacting one or more species in at least two of the active regions in a sequential process, e.g., sequential reactions. The device has a transparent substrate member, which has a surface region. The device has a silane material overlying the surface region. The device has a first active region formed overlying a first portion of the silane material. In a specific embodiment, the first active region has a first dimension of less than 1 micron in size and has one or more first molecules capable of binding to the first portion of the silane material. In a specific embodiment, the device has a second active region formed overlying a second portion of the silane material. The second active region has a second dimension of less than 1 micron in size and has one or more second molecules capable of binding to the second portion of the active region.Type: ApplicationFiled: November 14, 2005Publication date: November 2, 2006Applicant: California Institute of TechnologyInventors: Lawrence Wade, Charles Collier
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Publication number: 20050269509Abstract: A method for fabricating assembled structures. The method includes providing a tip structure, which has a first end, a second end, and a length defined between the first end and the second end. The second end is a free end. The method includes attaching a nano-sized structure along a portion of the length of the tip structure to extend a total length of the tip structure to include the length of the tip structure and a first length associated with the nano-sized structure. The method includes shortening the nano-sized structure from the first length to a second length. The method also includes pushing the nano-sized structure in a direction parallel to the second length to reduce the second length to a third length of the nano-sized structure along the direction parallel to the second length to cause the nano-sized structure to move along a portion of the length of the tip structure.Type: ApplicationFiled: January 27, 2005Publication date: December 8, 2005Applicant: California Institute of TechnologyInventors: Charles Collier, Ma Ziyang, Stephen Quake, Ian Shapiro, Lawrence Wade
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Patent number: 6953927Abstract: Methods and systems for operating an apertureless microscope for observing one or more features to a molecular sensitivity on objects are described. More particularly, the method includes moving a tip of a probe coupled to a cantilever in a vicinity of a feature of a sample, which emits one or more photons at a detected rate relative to a background rate of the sample based upon the presence of the tip of the probe in the vicinity of the feature. The method modifies the detected rate of the feature of the sample, whereupon the modifying of the detected rate causes the feature of the sample to enhance relative to background rate of the feature.Type: GrantFiled: July 9, 2003Date of Patent: October 11, 2005Assignee: California Institute of TechnologyInventors: Stephen R. Quake, Guillaume Lessard, Lawrence A. Wade, Jordan M. Gerton
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Publication number: 20050191427Abstract: Embodiments in accordance with the present invention relate to techniques for the growth and attachment of single wall carbon nanotubes (SWNT), facilitating their use as robust and well-characterized tools for AFM imaging and other applications. In accordance with one embodiment, SWNTs attached to an AFM tip can function as a structural scaffold for nanoscale device fabrication on a scanning probe. Such a probe can trigger, with nanometer precision, specific biochemical reactions or conformational changes in biological systems. The consequences of such triggering can be observed in real time by single-molecule fluorescence, electrical, and/or AFM sensing. Specific embodiments in accordance with the present invention utilize sensing and manipulation of individual molecules with carbon nanotubes, coupled with single-molecule fluorescence imaging, to allow observation of spectroscopic signals in response to mechanically induced molecular changes.Type: ApplicationFiled: February 20, 2004Publication date: September 1, 2005Applicant: California Institute of TechnologyInventors: Lawrence Wade, Ian Shapiro, Charles Collier, Maria Esplandiu, Vern Bittner, Konstantinos Giapis
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Publication number: 20040089816Abstract: Methods and systems for operating an apertureless microscope for observing one or more features to a molecular sensitivity on objects are described. More particularly, the method includes moving a tip of a probe coupled to a cantilever in a vicinity of a feature of a sample, which emits one or more photons at a detected rate relative to a background rate of the sample based upon the presence of the tip of the probe in the vicinity of the feature. The method modifies the detected rate of the feature of the sample, whereupon the modifying of the detected rate causes the feature of the sample to enhance relative to background rate of the feature.Type: ApplicationFiled: July 9, 2003Publication date: May 13, 2004Applicant: California Institute of TechnologyInventors: Stephen R. Quake, Guillaume Lessard, Lawrence A. Wade, Jordan M. Gerton