Patents by Inventor Lawrence E. Pelcher

Lawrence E. Pelcher has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 5474929
    Abstract: Adenosine deaminase gene (ADA gene) is used as a selectable/reporter gene for plant cells and plants. When introduced into plant cells, the ADA gene makes the cells resistant to inhibition by 2-deoxyadenosine or an analog, so transformed cells can be separated from non-transformed cells by culturing a cell mixture in a medium containing 2-deoxyadenosine or an analog in amounts that inhibit normal plant cells. A single vector may be constructed containing an ADA gene from any source (e.g. a mouse ADA gene) and an additional gene-of-interest to be introduced into plant cells. The vector may then be introduced into the cells by such a route as Agrobacterium infection or micro-projectile bombardment. The presence of the ADA gene in successfully transformed plant cells may be confirmed by cultivating the cells in a medium containing 2-deoxyadenosine or an analog in the presence of a material that changes color in the presence of ammonia. Observation of a color change confirms the presence of the gene.
    Type: Grant
    Filed: October 28, 1993
    Date of Patent: December 12, 1995
    Assignee: National Research Council of Canada
    Inventor: Lawrence E. Pelcher