Abstract: Disclosed herein are methods of performing continuous directed evolution in complex biological systems, including metazoan cells. These methods include the infection of engineered, non-naturally occurring metazoan cells with engineered, non-naturally occurring DNA viruses. The generation of infectious viruses that can infect new cells depends on the evolution of a gene of interest which is driven by an error-prone adenoviral polymerase. Also disclosed herein, are the compositions of engineered, non-naturally occurring metazoan cells and engineered, non-naturally occurring DNA viruses that function as components in the continuous directed evolution methodologies.

Abstract: The present disclosure relates to engineered erythroid cells and enucleated cells that include one or more of exogenous HLA-G polypeptides, exogenous immunogenic polypeptides, and exogenous coinhibitory polypeptides wherein the cells are capable of inducing immune tolerance and/or reducing immune response to the exogenous immunogenic polypeptides when administered to a subject.

Abstract: The present disclosure provides customizable enucleated erythroid cells or enucleated cells that can be engineered to include, on their surface, a loadable exogenous antigen-presenting polypeptide, wherein the loadable exogenous antigen-presenting polypeptide comprises one or more amino acid substitutions. In some embodiments, the one or more amino acid substitutions stabilize the loadable exogenous antigen-presenting polypeptide on the cell surface. In some embodiments, the loadable exogenous antigen-presenting polypeptide is stabilized on the cell surface in the absence of a polypeptide bound to the loadable exogenous antigen-presenting polypeptide. In some embodiments, the loadable exogenous antigen-presenting polypeptide comprises an exogenous displaceable polypeptide bound to the loadable exogenous antigen-presenting polypeptide. In some embodiments, the loadable exogenous antigen-presenting polypeptide is stabilized on the cell surface upon release of the displaceable polypeptide.

Abstract: Disclosed herein are methodologies and kits for dynamic targeted hypermutation that harness the enzymatic activity of a polynucleic acid-binding protein fused to a nucleobase-editing enzyme to specifically target mutations across a region of interest. These methodologies and kits facilitate the rapid creation of diverse DNA libraries in vivo or in vitro.

Abstract: Disclosed herein are methods of performing continuous directed evolution in complex biological systems, including metazoan cells. These methods include the infection of engineered, non-naturally occurring metazoan cells with engineered, non-naturally occurring DNA viruses. The generation of infectious viruses that can infect new cells depends on the evolution of a gene of interest which is driven by an error-prone adenoviral polymerase. Also disclosed herein, are the compositions of engineered, non-naturally occurring metazoan cells and engineered, non-naturally occurring DNA viruses that function as components in the continuous directed evolution methodologies.

Abstract: An apparatus is disclosed that forms a piping thread form of four threads per inch pipe (TPI). The thread form is configured to have a load flank, a load flank angle, a stab flank, a stab flank angle, a crest, and a root. Hydrostatic pressure tests using the 4-round thread form at pressures of 4,500 psi and greater resulted in failures due to thread leaks. Tensile tests using the 4-round thread form at loads of 187,400 lbs. and greater resulted in shearing at forces of 3,535 psi and greater.

Abstract: A method for reviewing a treatment plan including displaying list of at least one of a selected plurality of patients, institutions, and treatment plans associated with a treatment planning system, selecting at least one of the one or more patients, institutions, and treatment plans, querying treatment plan parameters associated with the selected one or more patients, institutions, and treatment plans, and generating a report file of the queried treatment plan parameters

Abstract: An apparatus is disclosed that forms a piping thread form of four threads per inch pipe (TPI). The thread form is configured to have a load flank, a load flank angle, a stab flank, a stab flank angle, a crest, and a root. Hydrostatic pressure tests using the 4-round thread form at pressures of 4,500 psi and greater resulted in failures due to thread leaks. Tensile tests using the 4-round thread form at loads of 187,400 lbs. and greater resulted in shearing at forces of 3,535 psi and greater.

Abstract: An x-ray diffractometry technique finds thickness of multiple layers of non-metallic crystalline material. A rocking curve is windowed to eliminate a main peak. The windowed curve is smoothed. The smoothed curve is subtracted from the windowed curve to yield a difference curve. The difference curve is transformed to make its average value zero and to constrain its endpoints to zero. A Fast Fourier transform is applied to the transformed difference curve. A thickness transform is applied to the result to yield a layer thickness.

Abstract: A process and apparatus to reclaim solid polystyrene-type polymer material in solid form comprising a dissolving section to dissolve the solid polystyrene-type polymer material in a solvent including a dissolving tank to receive solvent from a solvent reservoir and the polystyrene-type polymer material from an external source to form a solution thereof and a recovery section including a recovery tank to receive the solution from the dissolving section, a separator unit to separate the dissolved polystyrene-type polymer material in solid form from the solvent, a solid material processing unit to remove the reclaimed polystyrene-type polymer material from the separator unit and a fluid separator/recovery unit to remove moisture from the separator unit and separate and recover the solvent from the water.

Abstract: A blow moulding method. Cold parisons are conveyed through an oven for heating and then stretched and blown in a mould. A transfer assembly, engages the upper end of each heated parison, removes it from the oven, and swings it into a mould and then rises to stretch the parison as a threading die holds the lower end thereof. The mould then closes and air is injected to form the bottle, after which the mould opens and the finished bottle is removed.

Abstract: A blow molding apparatus including a rotary frame located adjacent a parison heating oven and having a plurality of blow molds mounted thereon for rotation therewith about the frame axis. A separate transfer arm is provided for each blow mold, the transfer arms also being located on the rotating frame for rotation therewith, each transfer arm being operable to transfer heated parisons from the oven to its respective blow mold.