Patents by Inventor Lawrence S. Cousens
Lawrence S. Cousens has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20030072747Abstract: The present invention provides purified and isolated polynucleotide sequences encoding human plasma platelet-activating factor acetylhydrolase. Also provided are materials and methods for the recombinant production of platelet-activating factor acetylhydrolase products which are expected to be useful in regulating pathological inflammatory events.Type: ApplicationFiled: October 13, 2001Publication date: April 17, 2003Applicant: ICOS CorporationInventors: Lawrence S. Cousens, Christine D. Eberhardt, Patrick Gray, Hai Le Trong, Larry W. Tjoelker, Cheryl L. Wilder
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Publication number: 20020146764Abstract: Novel methods and compositions are provided for enhanced yield of heterologous proteins in eucaryotic cells. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide. In particular, a sequence coding for ubiquitin is joined to another polypeptide of interest providing for high yields of the fusion product.Type: ApplicationFiled: August 16, 2001Publication date: October 10, 2002Applicant: Chiron CorporationInventors: Lawrence S. Cousens, Patricia A. Tekamp-Olson, Jeffrey R. Shuster, James P. Merryweather
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Publication number: 20020028481Abstract: Novel methods and compositions are provided for enhanced yield of heterologous proteins in eucaryotic cells. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide. In particular, a sequence coding for ubiquitin is joined to another polypeptide of interest providing for high yields of the fusion product.Type: ApplicationFiled: May 24, 1995Publication date: March 7, 2002Inventors: LAWRENCE S. COUSENS, PATRICIA A. TEKAMP-OLSON, JEFFREY R. SHUSTER, JAMES P. MERRYWEATHER
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Publication number: 20010021379Abstract: The present invention provides purified and isolated polynucleotide sequences encoding human plasma platelet-activating factor acetylhydrolase. Also provided are materials and methods for the recombinant production of platelet-activating factor acetylhydrolase products which are expected to be useful in regulating pathological inflammatory events.Type: ApplicationFiled: December 4, 2000Publication date: September 13, 2001Applicant: ICOS CorporationInventors: Lawrence S. Cousens, Christine D. Eberhardt, Patrick Gray, Hai Le Trong, Larry W. Tjoelker, Cheryl L. Wilder
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Patent number: 6203790Abstract: The present invention provides purified and isolated polynucleotide sequences encoding human plasma platelet-activating factor acetylhydrolase. Also provided are materials and methods for the recombinant production of platelet-activating factor acetylhydrolase products which are expected to be useful in regulating pathological inflammatory events.Type: GrantFiled: May 23, 2000Date of Patent: March 20, 2001Assignee: ICOS CorporationInventors: Lawrence S. Cousens, Christine D. Eberhardt, Patrick Gray, Hai Le Trong, Larry W. Tjoelker, Cheryl L. Wilder
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Patent number: 6146625Abstract: The present invention provides purified and isolated polynucleotide sequences encoding human plasma platelet-activating factor acetylhydrolase. Also provided are materials and methods for the recombinant production of platelet-activating factor acetylhydrolase products which are expected to be useful in regulating pathological inflammatory events.Type: GrantFiled: January 22, 1998Date of Patent: November 14, 2000Assignee: ICOS CorporationInventors: Lawrence S. Cousens, Christine D. Eberhardt, Patrick Gray, Hai Le Trong, Larry W. Tjoelker, Cheryl L. Wilder
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Patent number: 6099836Abstract: The present invention provides purified and isolated polynucleotide sequences encoding human plasma platelet-activating factor acetylhydrolase. Also provided are materials and methods for the recombinant production of platelet-activating factor acetylhydrolase products which are expected to be useful in regulating pathological inflammatory events. Specifically disclosed are methods for the treatment of reperfusion injury using platelet-activating factor acetylhydrolase products.Type: GrantFiled: June 19, 1998Date of Patent: August 8, 2000Assignee: ICOS CorporationInventors: Lawrence S. Cousens, Christine D. Eberhardt, Patrick Gray, Hai Le Trong, Larry W. Tjoelker, Cheryl L. Wilder
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Patent number: 6045794Abstract: The present invention provides purified and isolated polynucleotide sequences encoding human plasma platelet-activating factor acetylhydrolase. Also provided are materials and methods for the recombinant production of platelet-activating factor acetylhydrolase products which are expected to be useful in regulating pathological inflammatory events.Type: GrantFiled: June 9, 1999Date of Patent: April 4, 2000Assignee: ICOS CorporationInventors: Lawrence S. Cousens, Christine D. Eberhardt, Patrick Gray, Hai Le Trong, Larry W. Tjoelker, Cheryl L. Wilder
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Patent number: 5977308Abstract: The present invention provides purified and isolated polynucleotide sequences encoding human plasma platelet-activating factor acetylhydrolase. Also provided are materials and methods for the recombinant production of platelet-activating factor acetylhydrolase products which are expected to be useful in regulating pathological inflammatory events.Type: GrantFiled: August 12, 1997Date of Patent: November 2, 1999Assignee: ICOS CorporationInventors: Lawrence S. Cousens, Christine D. Eberhardt, Patrick Gray, Hai Le Trong, Larry W. Tjoelker, Cheryl L. Wilder
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Patent number: 5866362Abstract: Protein aggregates obtained by a process comprising culturing a host cell that expresses a desired protein in a medium comprising an effective amount of Cu.sup.++ and wherein the desired protein forms inclusion bodies in the host cell.Type: GrantFiled: June 7, 1995Date of Patent: February 2, 1999Assignee: Chiron CorporationInventors: Lawrence S. Cousens, Patricia Tekamp-Olson
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Patent number: 5847088Abstract: The present invention provides purified and isolated polynucleotide sequences encoding human plasma platelet-activating factor acetylhydrolase. Also provided are materials and methods for the recombinant production of platelet-activating factor acetylhydrolase products which are expected to be useful in regulating pathological inflammatory events.Type: GrantFiled: June 7, 1995Date of Patent: December 8, 1998Assignee: ICOS CorporationInventors: Lawrence S. Cousens, Christine D. Eberhardt, Patrick Gray, Hai Le Trong, Larry W. Tjoelker, Cheryl L. Wilder
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Patent number: 5698403Abstract: The present invention provides purified and isolated polynucleotide sequences encoding human plasma platelet-activating factor acetylhydrolase. Also provided are materials and methods for the recombinant production of platelet-activating factor acetylhydrolase products which are expected to be useful in regulating pathological inflammatory events.Type: GrantFiled: June 7, 1995Date of Patent: December 16, 1997Assignee: ICOS CorporationInventors: Lawrence S. Cousens, Christine D. Eberhardt, Patrick Gray, Hai Le Trong, Larry W. Tjoelker, Cheryl L. Wilder
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Patent number: 5656431Abstract: The present invention provides purified and isolated polynucleotide sequences encoding human plasma platelet-activating factor acetylhydrolase. Also provided are materials and methods for the recombinant production of platelet-activating factor acetylhydrolase products which are expected to be useful in regulating pathological inflammatory events.Type: GrantFiled: June 7, 1995Date of Patent: August 12, 1997Assignee: ICOS CorporationInventors: Lawrence S. Cousens, Christine D. Eberhardt, Patrick Gray, Hai Le Trong, Larry W. Tjoelker, Cheryl L. Wilder
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Patent number: 5641669Abstract: The present invention provides purified and isolated polynucleotide sequences encoding human plasma platelet-activating factor acetylhydrolase. Also provided are materials and methods for the recombinant production of platelet-activating factor acetylhydrolase products which are expected to be useful in regulating pathological inflammatory events.Type: GrantFiled: October 6, 1994Date of Patent: June 24, 1997Assignee: ICOS CorporationInventors: Lawrence S. Cousens, Christine D. Eberhardt, Patrick Gray, Hai Le Trong, Larry W. Tjoelker, Cheryl L. Wilder
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Patent number: 5605801Abstract: The present invention provides purified and isolated polynucleotide sequences encoding human plasma platelet-activating factor acetylhydrolase. Also provided are materials and methods for the recombinant production of platelet-activating factor acetylhydrolase products which are expected to be useful in regulating pathological inflammatory events. Furthermore provided are therapeutic and diagnostic methods using such polynucleotide sequences and platelet-activating factor acethylhydrolase products.Type: GrantFiled: June 7, 1995Date of Patent: February 25, 1997Assignee: ICOS CorporationInventors: Lawrence S. Cousens, Christine D. Eberhardt, Patrick Gray, Hai L. Trong, Larry W. Tjoelker, Cheryl L. Wilder
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Patent number: 5532152Abstract: The present invention provides purified and isolated polynucleotide sequences encoding human plasma platelet-activating factor acetylhydrolase. Also provided are materials and methods for the recombinant production of platelet-activating factor acetylhydrolase products which are expected to be useful in regulating pathological inflammatory events.Type: GrantFiled: June 6, 1995Date of Patent: July 2, 1996Assignee: ICOS CorporationInventors: Lawrence S. Cousens, Christine D. Eberhardt, Patrick Gray, Larry W. Tjoelker, Cheryl L. Wilder, Hai Trong
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Patent number: 5523215Abstract: A method for isolating a desired protein which comprises: providing a host cell expressing the desired protein as an insoluble aggregate; culturing the host cell with an effective mount of Cu.sup.++ ; disrupting the host cell producing a lysate; incubating the insoluble fraction non-disulfide-bond reducing or non-copper competing chaotropic conditions to solubilize contaminants; separating the insoluble from the soluble fraction; and exposing the insoluble fraction to disulfide-bond reducing or copper competing chaotropic conditions to solubilize the desired protein.Type: GrantFiled: April 16, 1992Date of Patent: June 4, 1996Assignee: Chiron CorporationInventors: Lawrence S. Cousens, Patricia Tekamp-Olson
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Patent number: 5342921Abstract: Novel methods and compositions are provided for enhanced yield of heterologous proteins in fungi. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide, where the two polypeptides are joined by a selectively cleavable linkage. In particular, a sequence coding for superoxide dismutase is joined to another polypeptide of interest at either terminus of the superoxide dismutase in a yeast expression vector under transcriptional control of an active promoter and the vector introduced into a yeast host and the host grown. High yields of the fusion product are obtained in this manner, where the fusion product can be selectively cleaved so as to produce both the superoxide dismutase and the other polypeptide in high yield.The S.Type: GrantFiled: March 29, 1991Date of Patent: August 30, 1994Assignee: Chiron CorporationInventors: Lawrence S. Cousens, Patricia A. Tekamp-Olson, Jeffrey R. Shuster, James P. Merryweather
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Patent number: 5015575Abstract: Methods and compositions are provided for efficient production of polypeptides having insulin activity. The proinsulin gene is joined to secretion and processing signals for expression and secretion in yeast. The product may be obtained in enhanced yield in the nutrient medium.Cell line pyinse was deposited at the A.T.C.C. on Apr. 6, 1983 and given Accession No. 20670.Type: GrantFiled: February 13, 1990Date of Patent: May 14, 1991Assignee: Chiron CorporationInventors: Anthony J. Brake, Lawrence S. Cousens, Mickey S. Urdea, Pablo D. T. Valenzuela
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Patent number: 4914026Abstract: Methods and compositions are provided for efficient production of polypeptides having insulin activity. The proinsulin gene is joined to secretion and processing signals for expession and secretion in yeast. The product may be obtained in enhanced yield in the nutrient medium.Cell line AB103(PYinsl) was deposited at the A.T.C.C. on Apr. 6, 1983 and given Accession No. 20,670.Type: GrantFiled: October 23, 1986Date of Patent: April 3, 1990Assignee: Chiron CorporationInventors: Anthony J. Brake, Lawrence S. Cousens, Mickey S. Urdea, Pablo D. T. Valenzuela