Patents by Inventor Le Cong

Le Cong has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 12280741
    Abstract: A control apparatus and a method for locking or unlocking a door include a smart key including a door locking and unlocking button provided thereon and configured for transmitting a control signal for locking or unlocking the door through a door locking and unlocking button; a smart key check module configured to determine whether the smart key is in a vehicle; and a door control module configured to determine whether the vehicle is in a started state or a turned-off state, determine whether to receive a control signal for locking or unlocking the door from the smart key according to a result of the determining whether the smart key is in the vehicle when the vehicle is in the started state, and control a corresponding door of the vehicle when receiving the control signal for locking or unlocking the door from the smart key.
    Type: Grant
    Filed: February 13, 2023
    Date of Patent: April 22, 2025
    Assignees: Hyundai Motor Company, Kia Corporation
    Inventors: Bing Le Cong, Jing Song Sun, Xu Qiang Sun
  • Publication number: 20250101468
    Abstract: The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues of organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provide dare methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.
    Type: Application
    Filed: June 11, 2024
    Publication date: March 27, 2025
    Applicants: The Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College
    Inventors: Feng ZHANG, Le CONG, Fei RAN
  • Publication number: 20250034594
    Abstract: The present disclosure provides recombineering-editing systems using CRISPR and recombination enzymes as well as methods, vectors, nucleic acid compositions, and kits thereof. The methods and systems provide means for altering target DNA, including genomic DNA in a host cell.
    Type: Application
    Filed: September 1, 2022
    Publication date: January 30, 2025
    Inventor: Le Cong
  • Patent number: 12168789
    Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems. In particular the present invention comprehends engineered new guide architectures and enzymes to be used in optimized Staphylococcus aureus CRISPR-Cas enzyme systems.
    Type: Grant
    Filed: January 26, 2023
    Date of Patent: December 17, 2024
    Assignees: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY, PRESIDENT AND FELLOWS OF HARVARD COLLEGE, UNIVERSITY OF TOKYO
    Inventors: Feng Zhang, Winston Yan, Osamu Nureki, Kaijie Zheng, Le Cong, Hiroshi Nishimasu, Fei Ran, Yinqing Li
  • Publication number: 20240209447
    Abstract: The present disclosure provides systems, methods, nucleic acids, and kits for barcoding and tracking cells based on CRISPR editing technologies.
    Type: Application
    Filed: April 20, 2022
    Publication date: June 27, 2024
    Inventors: Le Cong, Nicholas W. Hughes
  • Patent number: 12018275
    Abstract: The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues of organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provide dare methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.
    Type: Grant
    Filed: February 25, 2020
    Date of Patent: June 25, 2024
    Assignees: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY, PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Feng Zhang, Le Cong, Fei Ran
  • Patent number: 11996168
    Abstract: The present invention relates to genomic informatics and gene-expression profiling. Gene-expression profiles provide complex molecular fingerprints regarding the relative state of a cell or tissue. Similarities in gene-expression profiles between organic states provide molecular taxonomies, classification, and diagnostics. Similarities in gene-expression profiles resulting from various external perturbations reveal functional similarities between these perturbagens, of value in pathway and mechanism-of-action elucidation. Similarities in gene-expression profiles between organic and induced states may identify clinically-effective therapies. Systems and methods herein provide for the measurement of relative gene abundances, including unbiased selection of and construction of probes and targets designed and methods for using known properties of sparsity of measurements to reach gene abundances.
    Type: Grant
    Filed: October 27, 2016
    Date of Patent: May 28, 2024
    Assignees: The Broad Institute, Inc., Massachusetts Institute of Technology
    Inventors: Aviv Regev, Eric S. Lander, Brian Cleary, Le Cong
  • Publication number: 20240051498
    Abstract: A control apparatus and a method for locking or unlocking a door include a smart key including a door locking and unlocking button provided thereon and configured for transmitting a control signal for locking or unlocking the door through a door locking and unlocking button; a smart key check module configured to determine whether the smart key is in a vehicle; and a door control module configured to determine whether the vehicle is in a started state or a turned-off state, determine whether to receive a control signal for locking or unlocking the door from the smart key according to a result of the determining whether the smart key is in the vehicle when the vehicle is in the started state, and control a corresponding door of the vehicle when receiving the control signal for locking or unlocking the door from the smart key.
    Type: Application
    Filed: February 13, 2023
    Publication date: February 15, 2024
    Applicants: Hyundai Motor Company, Kia Corporation
    Inventors: Bing Le CONG, Jing Song Sun, Xu Qiang Sun
  • Publication number: 20230287373
    Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRJSPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CR.ISPR-Cas enzyme systems. In particular the present invention comprehends engineered new guide architectures and enzymes to be used in optimized Staphylococcus aureus CRISPR-Cas enzyme systems.
    Type: Application
    Filed: January 26, 2023
    Publication date: September 14, 2023
    Applicants: The Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College, University of Tokyo
    Inventors: Feng ZHANG, Winston YAN, Osamu NUREKI, Kaijie ZHENG, Le CONG, Hiroshi NISHIMASU, Fei RAN, Yinqing LI
  • Publication number: 20230091242
    Abstract: The present disclosure provides recombineering-editing systems using CRISPR and recombination enzymes as well as methods, vectors, nucleic acid compositions, and kits thereof. The methods and systems provide means for altering target DNA, including genomic DNA in a host cell.
    Type: Application
    Filed: March 2, 2021
    Publication date: March 23, 2023
    Inventor: Le Cong
  • Patent number: 11578312
    Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems. In particular the present invention comprehends engineered new guide architectures and enzymes to be used in optimized Staphylococcus aureus CRISPR-Cas enzyme systems.
    Type: Grant
    Filed: December 12, 2017
    Date of Patent: February 14, 2023
    Assignees: THE BROAD INSTITUTE INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY, PRESIDENT AND FELLOWS OF HARVARD COLLEGE, UNIVERSITY OF TOKYO
    Inventors: Feng Zhang, Winston Yan, Osamu Nureki, Kaijie Zheng, Le Cong, Hiroshi Nishimasu, Fei Ran, Yinqing Li
  • Publication number: 20230029506
    Abstract: The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.
    Type: Application
    Filed: June 3, 2022
    Publication date: February 2, 2023
    Applicants: The Broad Institute, Inc., Massachusetts Institute of Technology
    Inventors: Le CONG, David Benjamin Turitz COX, Matthias HEIDENREICH, Randall Jeffrey PLATT, Lukasz SWIECH, Feng ZHANG
  • Patent number: 11434262
    Abstract: Provided herein are compositions, kits and methods useful in the construction of designer transcription activator-like effector (dTALE) polypeptides.
    Type: Grant
    Filed: October 26, 2016
    Date of Patent: September 6, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: Feng Zhang, Le Cong, Sriram Kosuri, George M. Church
  • Patent number: 11407985
    Abstract: The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: August 9, 2022
    Assignees: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Le Cong, David Benjamin Turitz Cox, Matthias Heidenreich, Randall Jeffrey Platt, Lukasz Swiech, Feng Zhang
  • Publication number: 20220204954
    Abstract: The disclosure provides variant Staphylococcus aureus Cas9 (SaCas9) proteins with altered specificity for protospacer adjacent motif (PAM) sequences. The disclosure also is directed to CRISPR/Cas9 systems and methods of altering a genomic DNA sequence using the variant SaCas9 protein. Methods of generating variant Cas9 proteins with altered PAM specificity are also disclosed.
    Type: Application
    Filed: April 24, 2020
    Publication date: June 30, 2022
    Inventor: Le Cong
  • Patent number: 11332719
    Abstract: The present invention generally relates to methods and compositions used delivery of gene editing compositions including transcriptional effectors with parvovirus and preferred methods for making same.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: May 17, 2022
    Assignees: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY, PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Feng Zhang, Mark D. Brigham, Le Cong, Silvana Konermann
  • Patent number: 11312937
    Abstract: The invention relates to methods of altering expression of a genomic locus of interest or specifically targeting a genomic locus of interest in an animal cell, which may involve contacting the genomic locus with a non-naturally occurring or engineered composition that includes a deoxyribonucleic acid (DNA) binding polypeptide having a N-terminal capping region, a DNA binding domain comprising at least five or more Transcription activator-like effector (TALE) monomers and at least one or more half-monomers specifically ordered to target the genomic locus of interest, and a C-terminal capping region, wherein the polypeptide includes at least one or more effector domains, and wherein the polypeptide is encoded by and translated from a codon optimized nucleic acid molecule so that the polypeptide preferentially binds to the DNA of the genomic locus.
    Type: Grant
    Filed: June 19, 2018
    Date of Patent: April 26, 2022
    Assignees: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY, PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Feng Zhang, Le Cong
  • Patent number: 11214797
    Abstract: The present invention provides tools and methods for the systematic analysis of genetic interactions, including higher order interactions. The present invention provides tools and methods for combinatorial probing of cellular circuits, for dissecting cellular circuitry, for delineating molecular pathways, and/or for identifying relevant targets for therapeutics development.
    Type: Grant
    Filed: April 27, 2018
    Date of Patent: January 4, 2022
    Assignees: The Broad Institute, Inc., Massachusetts Institute of Technology, The Regents of the University of California
    Inventors: Aviv Regev, Brittany Adamson, Brian Cleary, Le Cong, Atray Dixit, Jellert Gaublomme, Eric S. Lander, Thomas Norman, Oren Parnas, Orit Rozenblatt-Rosen, Alexander K. Shalek, Jonathan Weissman
  • Publication number: 20210403861
    Abstract: The invention relates to methods of altering expression of a genomic locus of interest or specifically targeting a genomic locus of interest in an animal cell, which may involve contacting the genomic locus with a non-naturally occurring or engineered composition that includes a deoxyribonucleic acid (DNA) binding polypeptide having a N-terminal capping region, a DNA binding domain comprising at least five or more Transcription activator-like effector (TALE) monomers and at least one or more half-monomers specifically ordered to target the genomic locus of interest, and a C-terminal capping region, wherein the polypeptide includes at least one or more effector domains, and wherein the polypeptide is encoded by and translated from a codon optimized nucleic acid molecule so that the polypeptide preferentially binds to the DNA of the genomic locus.
    Type: Application
    Filed: September 16, 2021
    Publication date: December 30, 2021
    Applicants: The Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College
    Inventors: Feng ZHANG, Le Cong
  • Patent number: 11186825
    Abstract: The present invention provides markers, marker signatures and molecular targets that correlate with dysfunction of immune cells and are advantageously independent of the immune cell activation status. The present markers, marker signatures and molecular targets provide for new ways to evaluate and modulate immune responses. Specifically, POU2AF1 modulation is provided for use as a marker, marker signature and molecular target. Therapeutic methods are also provided to treat a patient in need thereof who would benefit from an increased immune response.
    Type: Grant
    Filed: April 30, 2018
    Date of Patent: November 30, 2021
    Assignees: The Broad Institute, Inc., Massachusetts Institute of Technology, The Brigham and Women's Hospital, Inc.
    Inventors: Aviv Regev, Ana Carrizosa Anderson, Le Cong, Vijay K. Kuchroo, Meromit Singer, Chao Wang