Patents by Inventor Le JI

Le JI has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Fermentation process with Pichia yeast expressing recombinant protein
    Patent number: 11104928
    Abstract: The present invention discloses a fermentation process with a Pichia yeast expressing a recombinant protein, in which Pichia pastoris is used as a fungal strain. The process comprises performing primary seed culturing to reach a fungal concentration of 20±2 g/L; then performing secondary seed culturing to reach a fungal concentration of 120±10 g/L; next proceeding to a glycerol culturing stage, wherein the amount of glycerol added in the glycerol culturing stage is 60-70 g/L; and then proceeding to a methanol-induced stage for 120±8 h after the dissolved oxygen quickly reaches a relatively stable state, to complete the fermentation process. In the present invention, a glycerol fed-batch addition stage in existing processes is omitted, and the process proceeds to a next stage as soon as glycerol is completely consumed, with no need to prepare sterilized glycerol for fluidic addition. As such, the glycerin sterilizer is omitted, the consumption of energy and resource and the waste of glycerin are reduced.
    Type: Grant
    Filed: October 18, 2016
    Date of Patent: August 31, 2021
    Assignee: JIANGSU JLAND BIOTECH CO., LTD.
    Inventors: Shulin Yang, Jianfeng Zhao, Jianmin Huang, Lihu Gao, Erfeng Du, Hai Tao, Liping Feng, Le Ji, Aimei Zhou
  • FERMENTATION PROCESS WITH PICHIA YEAST EXPRESSING RECOMBINANT PROTEIN
    Publication number: 20190309339
    Abstract: The present invention discloses a fermentation process with a Pichia yeast expressing a recombinant protein, in which Pichia pastoris is used as a fungal strain. The process comprises performing primary seed culturing to reach a fungal concentration of 20±2 g/L; then performing secondary seed culturing to reach a fungal concentration of 120±10 g/L; next proceeding to a glycerol culturing stage, wherein the amount of glycerol added in the glycerol culturing stage is 60-70 g/L; and then proceeding to a methanol-induced stage for 120±8 h after the dissolved oxygen quickly reaches a relatively stable state, to complete the fermentation process. In the present invention, a glycerol fed-batch addition stage in existing processes is omitted, and the process proceeds to a next stage as soon as glycerol is completely consumed, with no need to prepare sterilized glycerol for fluidic addition. As such, the glycerin sterilizer is omitted, the consumption of energy and resource and the waste of glycerin are reduced.
    Type: Application
    Filed: October 18, 2016
    Publication date: October 10, 2019
    Applicant: JIANGSU JLAND BIOTECH CO., LTD.
    Inventors: Shulin YANG, Jianfeng ZHAO, Jianmin HUANG, Lihu GAO, Erfeng DU, Hai TAO, Liping FENG, Le JI, Aimei ZHOU