Abstract: Methods for transdermal delivery of glycosaminoglycans (GAGs) are disclosed, comprising applying ultrasound to the skin followed by topical administration of one or more GAGs optionally complexed with a polysaccharide carrier. When GAG is hyaluronic acid complexed with a modified starch, a transdermal delivery method is provided for facilitating delivery of high molecular weight hyaluronic acid into deep layers of the epidermis and to the dermis in a non-invasive, convenient and painless way. This transdermal delivery method may be applied in treatment of skin aging phenomena associated with collagen and/or hyaluronic acid depletion or loss.