Abstract: The present invention is related to a treatment unit (3) having an inlet (3a) and an outlet (3b), and a treatment space (4) being formed inside the treatment unit (3) such that it can be penetrated by electric pulses, said treatment space (4) being fluidly connected with the inlet (3a) and the outlet (3b), wherein at least a portion of an inner side face of the treatment space (4) is curved. The present invention is furthermore related to a device comprising such a treatment unit and to a method to be performed with said device, and wherein the inlet (3a) is formed in a top face of the treatment unit (3), and the outlet (3b) is formed in a bottom face of the treatment unit (3).

Abstract: The present invention is related to a treatment unit (3) having an inlet (3a) and an outlet (3b), and a treatment space (4) being formed inside the treatment unit (3) such that it can be penetrated by electric pulses, said treatment space (4) being fluidly connected with the inlet (3a) and the outlet (3b), wherein at least a portion of an inner side face of the treatment space (4) is curved. The present invention is furthermore related to a device comprising such a treatment unit and to a method to be performed with said device, and wherein the inlet (3a) is formed in a top face of the treatment unit (3), and the outlet (3b) is formed in a bottom face of the treatment unit (3).

Abstract: The invention relates to a method of making a product for human consumption which comprises partially lysed microalgae, said method comprising a) preparing a suspension of microalgae, wherein the microalgae belong to a phylaselected from Chlorophyta, Ochrophyta, and Heterokonta; b) applying a pulsed electric field to the suspension of microalgae, wherein said pulsed electric field has a specific energy input between 25 to 150 kJ per kg suspension (kg sus?1); c) optionally treating the microalgae with enzyme; d) incubating the biomass suspension at 300 rpm, 4-37° C. for 6-48 h e) forming a biomass of partially lysed microalgae; and f) adding the biomass of partially lysed microalgae to a product for human consumption.

Abstract: The present invention is related to a method for the identification of optimized conditions for treating cells with electric pulses for targeted inactivation, the extraction of bioactive compounds, and the stimulation of cell growth and/or cellular compounds, comprising the steps of treating samples comprising cellular material under at least one condition, preferably two or more different conditions, analysing the results of the treatment in step a) for each of the applied different conditions, and identifying suitable conditions from the analysis of step b). The present invention is furthermore related to a system (1) for performing said method, as well as to a method for treating cells for targeted inactivation, the extraction of bioactive compounds, and the stimulation of cell growth and/or cellular compounds, employing the above identified suitable conditions.

Abstract: The invention relates to an industrial bioreactor with a dual cycle-controlled optimization process providing an optimized cultivation process for cell cultures, cell components or metabolic products of the cells in a nutrient medium. The bioreactor comprises a reactor vessel providing controlled bioreactor conditions for the cultivation process, a control unit connected to sensory devices measuring sensory parameter values comprising at least measures related to the composition of the nutrient medium and/or concentration of the nutrient medium and/or oxygen and/or temperature and/or pH-value and/or sterility, and transmitting them to the control unit. The control unit controls and automatically optimizes the operational parameter of the cultivation process and operational parameters of a treatment process applied to the cells during the cultivation process by adjusting operational parameters of the bioreactor affecting the measured sensory parameter values.

Abstract: The present invention is related to an insert for a device for treating cells, wherein said insert comprises electrodes for emitting electric pulses and has an electrical connection, wherein a treatment space is provided in said insert, wherein said treatment space can be penetrated by the electric pulses emitted by said electrodes and an electric field resulting therefrom. The present invention is also related to such a device and to a method performed with said device.

Abstract: The present invention is related to an insert for a device for treating cells, wherein said insert comprises electrodes for emitting electric pulses and has an electrical connection, wherein a treatment space is provided in said insert, wherein said treatment space can be penetrated by the electric pulses emitted by said electrodes and an electric field resulting therefrom. The present invention is also related to such a device and to a method performed with said device.

Abstract: The present invention relates to methods of increasing metabolic activity and stimulating cell proliferation of biological cells through the use of pulsed electric field (PEF) treatment, especially through the use of nanosecond pulsed electric field (nsPEF) treatment.

Abstract: A device, comprising a unit for taking up a fluid, a unit for emitting electric pulses, wherein said unit for taking up a fluid and said unit for emitting electric pulses are connected with each other via lines so as to form a circuit, wherein there are provided connections for connecting the lines arranged at said unit for taking up a fluid with the lines arranged at said unit for emitting electric pulses in a releasable, hygienic and leakage-free manner. An apparatus comprising the aforementioned device, and a method for treating cells performed with the device or the apparatus, are also disclosed.

Abstract: The present invention relates to a device (1) for treating cells for stimulating cell growth, comprising a unit (2) for generating and emitting electric pulses, and a treatment unit (3) having an inlet (3a) and an outlet (3a), a treatment space (4) being formed inside the treatment unit (3), the treatment space (4) being penetrated by the emitted electric pulses and an electric field resulting therefrom, a cell material entering into the treatment space (4) through the inlet (3a) being moved without contact through the treatment space (4) and the electric field penetrating the treatment space (4) to the outlet (3b).

Abstract: The present invention is related to a device (1), comprising a unit (2) for generating and emitting electric pulses, two or more electrodes or plates (2a, 2b) of a capacitor that are arranged in one electric circuit with said unit (2), and a treatment space (3) arranged between said electrodes or plates (2a, 2b) so that the treatment space (3) can be penetrated by the emitted electric pulses and an electric field resulting therefrom, characterized in that at least one adjustable resistor (9) is arranged in said electric circuit.

Abstract: The present invention is related to a treatment unit (3) having an inlet (3a) and an outlet (3b), and a treatment space (4) being formed inside the treatment unit (3) such that it can be penetrated by electric pulses, said treatment space (4) being fluidly connected with the inlet (3a) and the outlet (3b), wherein at least a portion of an inner side face of the treatment space (4) is curved. The present invention is furthermore related to a device comprising such a treatment unit and to a method to be performed with said device, and wherein the inlet (3a) is formed in a top face of the treatment unit (3), and the outlet (3b) is formed in a bottom face of the treatment unit (3).

Abstract: The present invention relates to a device (1) for treating cells for stimulating cell growth, comprising a unit (2) for generating and emitting electric pulses and an electric field resulting therefrom, and a treatment space (3) that can be penetrated by the emitted electric pulses, wherein the device (1) further comprises a unit (4, 9) which can be moved into and out of said treatment space (3) such that cell material provided within a compartment (4a) of said unit (4), or provided in a container (5) on or in said unit (4, 9), can be moved into and out of said treatment space (3), and wherein said treatment space (3) is provided between two electrodes (2a, 2b) or plates of a capacitor parallel to each other.