Abstract: The invention provides a method for controlling Huanglongbing (HLB) disease of citrus plants through expressing genes encoding synthases for sesquiterpenes such as ?-caryophyllene, and ?-copaene, and combinations thereof, in citrus plants. Methods of controlling HLB comprising applying at least one purified sesquiterpene, which repels Diaphorina citri and/or Tryoza erytrae psyllid insects, so as to control the HLB disease of citrus plants, are also disclosed.

Abstract: The invention provides a method for controlling Huanglongbing (HLB) disease of citrus plants through expressing genes encoding synthases for sesquiterpenes such as ?-caryophyllene, and ?-copaene, and combinations thereof, in citrus plants. Methods of controlling HLB comprising applying at least one purified sesquiterpene, which repels Diaphorina citri and/or Tryoza erytrae psyllid insects, so as to control the HLB disease of citrus plants, are also disclosed.

Abstract: The invention provides a method for controlling Huanglongbing (HLB) disease of citrus plants through expressing genes encoding synthases for sesquiterpenes such as ?-caryophyllene, and ?-copaene, and combinations thereof, in citrus plants. Methods of controlling HLB comprising applying at least one purified sesquiterpene, which repels Diaphorina citri and/or Tryoza erytrae psyllid insects, so as to control the HLB disease of citrus plants, are also disclosed.

Abstract: The invention provides a method for controlling Huanglongbing (HLB) disease of citrus plants through expressing genes encoding synthases for sesquiterpenes such as ?-caryophyllene, and ?-copaene, and combinations thereof, in citrus plants. Methods of controlling HLB comprising applying at least one purified sesquiterpene, which repels Diaphorina citri and/or Tryoza erytrae psyllid insects, so as to control the HLB disease of citrus plants, are also disclosed.

Abstract: The invention consists in modifying the levels of accumulation and emission of monoterpenes and sesquiterpenes in citrus as a mechanism to achieve systemic resistance against pathogens or repellency against pests. The alteration of the content of d-limonene and other terpenes is achieved by genetic transformation via the introduction of a gene that encodes an enzyme with d-limonene synthase activity, from a citrus fruit or plant or from another living organism, in antisense or RNAi (RNA interference) configuration. Genetic modification is achieved either by Agrobacterium tumefaciens or any other method of genetic transformation of plants from protoplasts or explants. The construction is incorporated in citrus genotypes or related genera of the family Rutaceae in order to reduce the levels of accumulation and emission of the monoterpene and precursor compounds and/or derivatives, either of leaves or flowers and/or fruit.

Abstract: The invention provides a method for controlling Huanglongbing (HLB) disease of citrus plants through expressing genes encoding synthases for sesquiterpenes such as ?-caryophyllene, and ?-copaene, and combinations thereof, in citrus plants. Methods of controlling HLB comprising applying at least one purified sesquiterpene, which repels Diaphorina citri and/or Tryoza erytrae psyllid insects, so as to control the HLB disease of citrus plants, are also disclosed.

Abstract: The invention consists in modifying the levels of accumulation and emission of monoterpenes and sesquiterpenes in citrus as a mechanism to achieve systemic resistance against pathogens or repellency against pests. The alteration of the content of d-limonene and other terpenes is achieved by genetic transformation via the introduction of a gene that encodes an enzyme with d-limonene synthase activity, from a citrus fruit or plant or from another living organism, in antisense or RNAi (RNA interference) configuration. Genetic modification is achieved either by Agrobacterium tumefaciens or any other method of genetic transformation of plants from protoplasts or explants. The construction is incorporated in citrus genotypes or related genera of the family Rutaceae in order to reduce the levels of accumulation and emission of the monoterpene and precursor compounds and/or derivatives, either of leaves or flowers and/or fruit.

Abstract: The procedure is based on the use, as vegetable starting material for the transformation, of the first shoots from the graft of buds of adult trees onto stock, the genetic transformation of explants from the adult shoots by mean of co cultivation with Agrobacterium tumefaciens in mother plaques, and the obtaining of complete adult woody plants by means of in vitro micrografting of the transgenic buds, apices or shoots, regenerated by means of the explants, by means of in vitro micrograft onto stock cultivated in vitro.This procedure makes it possible to avoid the juvenile period and the high heterozygosis which affects most woody species, blossoming and fruiting of the transgenic plants are brought forward, and it permits the direct genetic transformation of commercially interesting varieties.It has argicultural applications.