Abstract: This invention relates to the differentiation of recombination activating gene (RAG) inactivated progenitor cells into T cells through the expression of an exogenous T Cell Receptor (TCR). A population of T cells may be produced by a method that comprises (i) differentiating a population of RAG inactivated induced pluripotent stem cells (IPSCs) into mesoderm cells, (ii) differentiating the mesoderm cells (MCs) to produce a population of haemogenic endothelial cells (HECs), (iii) differentiating the HECs into a population of haematopoietic progenitor cells (HPCs), (iv) differentiating the population of HECs into progenitor T cells; and (v) maturing the progenitor T cells to produce a population of double positive CD4+CD8+ T cells. The method may further comprise introducing a heterologous nucleic acid encoding an antigen receptor, such as a T Cell Receptor (TCR) or chimeric antigen receptor (CAR), into one of the RAG inactivated (a) IPSCs (b) MCs (c) HECs (d) HPCs or (e) the progenitor T cells.

Abstract: This invention concerns chemically defined haematopoietic induction media that support the differentiation of haemogenic endothelial cells (HECs) into haematopoietic progenitor cells (MFCs) that are capable of further differentiation into T cells. The media may (a) stimulate cKIT receptor and/or cKIT receptor mediated signalling pathways and/or (b) stimulate VEGFR and/or VEGFR mediated signalling pathways. For example, the medium may comprise SCF and VEGF. In some embodiments, the media may further (c) stimulate MPL or MPL mediated signalling pathways; (d) stimulate FLT3 or FLT3 mediated signalling pathways (e) stimulate IGF1R or IGF1R mediated signalling pathways and (f) display interleukin (IL) activity. For example, the medium may further comprise Thrombopoietin (TPO), Fits ligand (FltSL), IGF-1, IL-3, IL-6 and optionally IL-7. These media may be useful for example in the production of blood cells or use in immunotherapy.

Abstract: This invention relates to the production of a population of haemogenic progenitor cells by (i) differentiating a population of induced pluri potent stem cells (IPSCs) into mesoderm cells and; (II) differentiating the mesoderm cells to produce a population of haemogenic progenitor cells. Steps (i) and (ii) are performed without purification or isolation of cells in the population. In addition, the haemogenic progenitor cells may be produced without the use of serum or stromal co-culture. Methods of the invention may be useful for example, in the production of clinical grade blood cells, such as T cells, for use in immunotherapy.

Abstract: A wall mount for an electronic device includes two screw or hook receiving keyhole apertures. The two keyhole apertures have different geometric shapes with respect to each other such that the locked mounting positions of the two corresponding mounting screws or hooks which would engage the same are neither laterally nor vertically aligned with each other.

Abstract: An antenna including a first conductive element having a first length and including a first connecting interface to connect, at a first end, the antenna structure to an electrical circuit, a second conductive element having a second length, the second conductive element connecting, at a first end, to a second end of the first conductive element, and a third conductive element having a third length and connecting at a first end to a second end of the second conductive element and oriented at an angle that is orthogonal to the orientation axis for the first conductive element and the second conductive element. The antenna can be placed by machine without the antenna moving or tilting in any direction prior attaching to a printed circuit board.

Abstract: A chipper has a knife clamped between a clamp and a counter knife that sits on a holder. The knife, damp, and counter knife are collectively positioned in a machined area formed in a holder that is in turn within a larger recess formed in a chipping disk or chipping drum. A first fastener secures the knife holder to the chipping disk, a second fastener secures the clamp to the knife holder, and a third fastener secures the counter knife lo the knife holder. The knife comprises a ridge which is formed by the termination in two straight lines which form a triangle shape on the bottom of the knife. These straight lines reduce the manufacturing cost of the knife and provide support for the knife to locate. The knife is also manufactured without 100% grinding of all services, which is unique in that this reduces manufacturing costs dramatically.

Abstract: Apparatus to wirelessly monitor a position of a hatch are disclosed. An example apparatus includes a hatch to be coupled to a fluid tank, a latch to secure the hatch in a closed position, and a sensor to detect whether the latch is securing the hatch in a closed position.

Abstract: A wall mount for an electronic device includes two screw or hook receiving keyhole apertures. The two keyhole apertures have different geometric shapes with respect to each other such that the locked mounting positions of the two corresponding mounting screws or hooks which would engage the same are neither laterally nor vertically aligned with each other.

Abstract: Apparatus providing a thermal interface includes a printed circuit board configured to be thermally coupled to a component to dissipate heat from the printed circuit board and including a surface to be thermally coupled to a surface of the component with a thermally conductive substance such as a thermal putty included in a region between the surface of the printed circuit board and the surface of the component and the surface of the printed circuit board includes a pattern of features configured to decrease migration of the thermal putty out of the region.

Abstract: An electronic device such as a set top box is provided having a vertical chassis wall having an aperture; a horizontal circuit board that extends toward the vertical chassis wall; F-connector connected to the horizontal circuit board, either under or over the board, and extending out of the a vertical chassis wall through the aperture; and inner shield, which can generally be used to contain/shield the RF circuit components mounted on the PCB, on the interior side of the vertical chassis wall and connected to the F-connector on or under the horizontal circuit board, wherein the inner shield comprises a series of vertical peripheral walls that surround components (either under or over the board); and a shield cover that covers the inner shield, the shield cover comprises a top portion that runs along or extends horizontally and side portions that extend from the periphery of the top portion and downward to overlap a part of the shield wall.

Abstract: The present principles of the embodiments generally relate to an apparatus and a method for bosses such as, for example, screw bosses, serving for multiple purposes. In one exemplary embodiment, a boss comprising a plurality of ribs is mated with a guide comprising a plurality of slots to resist twisting motion of the screw boss when a screw is inserted into the screw boss.

Abstract: An antenna including a first conductive element having a first length and including a first connecting interface to connect, at a first end, the antenna structure to an electrical circuit, a second conductive element having a second length, the second conductive element connecting, at a first end, to a second end of the first conductive element, and a third conductive element having a third length and connecting at a first end to a second end of the second conductive element and oriented at an angle that is orthogonal to the orientation axis for the first conductive element and the second conductive element. The antenna can be placed by machine without the antenna moving or tilting in any direction prior attaching to a printed circuit board.

Abstract: An improved heat transfer system for components an electronic device is provided. The electronic device includes a printed circuit board, a component shield and a heatsink or heat spreader. An open heat transfer window is positioned in the component shield so as to enable the heat sink to directly contact a thermal pad for a component requiring heat dissipation. Grounding fingers between the shield and the heatsink may be provided to connect the shield to the heatsink, thereby protecting the component against electromagnetic interference and electrostatic discharge from the outside while preventing the component from leaking radio frequency radiation to the outside resulting from the creation of the open heat transfer window in the shield.

Abstract: An improved heat transfer system for components an electronic device is provided. The electronic device includes a printed circuit board, a component shield and a heatsink or heat spreader. An open heat transfer window is positioned in the component shield so as to enable a depression in the heat sink to pass through the window and directly contact a thermal pad for a component requiring heat dissipation. A grounding connection between the shield and the heatsink is provided to prevent radiation loss in the radio frequency shielding capability resulting from the creation of the open heat transfer window in the shield.

Abstract: There is provided an electronic device. The electronic device includes a circuit board having heat generating components thereon. The electronic device further includes spring clips or bend tabs. The electronic device also includes a heat sink, disposed over the circuit board, through which heat from the circuit board and the components thereon is released. The heat sink has a generally flat horizontal base and a series of vertically directed fins. The heat sink is secured to at least one of the circuit board and at least one of the components thereon by the spring clips or bend tabs being positioned at a periphery of the horizontal base. The spring clips or bend tabs have distal contact prongs that contact and apply downward force on the horizontal base.

Abstract: A light pipe bracket (6) for an electronic device is provided that comprises a planar main body, light pipes (13) each having a proximal end and a distal end in which the light pipe extends from the distal end at the planar main body toward the distal end, and circuit board holding means (7) that support at least a portion of a circuit in the electronic device.

Abstract: There is provided an electronic device. The electronic device includes a circuit board having heat generating components thereon. The electronic device further includes spring clips or bend tabs. The electronic device also includes a heat sink, disposed over the circuit board, through which heat from the circuit board and the components thereon is released. The heat sink has a generally flat horizontal base and a series of vertically directed fins. The heat sink is secured to at least one of the circuit board and at least one of the components thereon by the spring clips or bend tabs being positioned at a periphery of the horizontal base. The spring clips or bend tabs have distal contact prongs that contact and apply downward force on the horizontal base.

Abstract: An electronic device such as a set top box is provided having a vertical chassis wall having an aperture; a horizontal circuit board that extends toward the vertical chassis wall; F-connector connected to the horizontal circuit board, either under or over the board, and extending out of the a vertical chassis wall through the aperture; and inner shield, which can generally be used to contain/shield the RF circuit components mounted on the PCB, on the interior side of the vertical chassis wall and connected to the F-connector on or under the horizontal circuit board, wherein the inner shield comprises a series of vertical peripheral walls that surround components (either under or over the board); and a shield cover that covers the inner shield, the shield cover comprises a top portion that runs along or extends horizontally and side portions that extend from the periphery of the top portion and downward to overlap a part of the shield wall.

Abstract: A light pipe bracket (6) for an electronic device is provided that comprises a planar main body, light pipes (13) each having a proximal end and a distal end in which the light pipe extends from the distal end at the planar main body toward the distal end, and circuit board holding means (7) that support at least a portion of a circuit in the electronic device.

Abstract: A screen using RNAi methods was used to test the entire set of protein kinases in Drosophila for an effect on mitosis. Most kinases previously known to be involved in the cell cycle were identified, providing validation of the approach. A mitotic function was found for a number of kinases not previously known to be involved in the cell cycle. Materials and methods are therefore provided for control of the cell cycle using modulators of expression or activity of kinases not previously known to act in mitosis, including human orthologues thereof.