Abstract: Provided is a method for determining a drug combination targeting different sites of a protein or a protein complex, including: identifying a first binding site and a second binding site of the protein or the protein complex based on a three-dimensional structure thereof, wherein the first binding site and the second binding site are different sites in the three-dimensional structure of the protein or the protein complex; identifying a first drug interacting with the first binding site; identifying a second drug interacting with the second binding site; and combining the first drug and the second drug to provide at least one of a synergistic effect and an additive effect in suppressing an activity of the protein or the protein complex. Also provided is a method for treating a ATG4B-related disease or a 3C-like protease-related disease by the drug combination.

Abstract: A drug ranking method, a drug ranking system and a comparison method for drug ranking method are provided. The processing unit executes ranking a plurality of drugs according to a protein target, comparing a plurality of drug ranking methods, and correcting a drug ranking method.

Abstract: Provided is a method for treating a SARS-CoV2 3CLpro-related disease in a subject in need thereof by blocking dimerization of 3C-like main protease (3CLpro) of the SARS-CoV2, including administering to the subject a first agent which binds to a first binding site of a SARS-CoV2 3CLpro complex and a second agent which binds to a second binding site of the SARS-CoV2 3CLpro complex, wherein the first binding site and the second binding site are functionally different sites in the three-dimensional structure of the SARS-CoV2 3CLpro complex. Also provided is a pharmaceutical combination including the first agent and the second agent for suppressing SARS-CoV2, thereby alleviating COVID-19.

Abstract: A text comparison method is adapted for comparing a query file with an existing file. The text comparison method includes: converting the existing file, by an irreversible method, to obtain a first intermediate file, wherein the first intermediate file includes a plurality of characters, and a number of different characters of the plurality of characters is a predetermined value; receiving a second intermediate file which is a file converted from the query file by the irreversible method; and according to a predetermined string length, comparing the second intermediate file with the first intermediate file by a high repeating-character comparison method to output a comparison result. Therefore, the second intermediate file can be created offline and then only the second intermediate file but not the original query file is submitted through internet for private text comparison.

Abstract: A text comparison method is adapted for comparing a query file with an existing file. The text comparison method includes: converting the existing file, by an irreversible method, to obtain a first intermediate file, wherein the first intermediate file includes a plurality of characters, and a number of different characters of the plurality of characters is a predetermined value; receiving a second intermediate file which is a file converted from the query file by the irreversible method; and according to a predetermined string length, comparing the second intermediate file with the first intermediate file by a high repeating-character comparison method to output a comparison result. Therefore, the second intermediate file can be created offline and then only the second intermediate file but not the original query file is submitted through interne for private text comparison.

Abstract: The present invention provides an evaluating system and the use thereof for the efficacy of antimicrobial peptide, which includes the following steps: (a) constructing a peptide by a first input unit, and load the peptide into an aqueous solution for a first time for equilibration; (b) constructing a lipid bilayer by a second input unit, and load the lipid bilayer into an aqueous solution for a second time for equilibration; (c) Using a first processing unit, simulations are carried out for an aqueous system containing an equilibrated peptide from the first input unit and the equilibrated lipid bilayer from the second input unit; (d) calculating the partition free energy of the peptide by a second processing unit; (e) outputting the prediction by an output unit, wherein the output unit is connected with the first processing unit and the second processing unit.

Abstract: The present invention relates to the area of ATG4-related disorders. More particularly, the present invention relates to a method of treating an ATG4B-related disorder comprising a step of administering a subject with an effective amount of tioconazole. The present invention relates also to a method of increasing a subject's responsiveness to a therapy for a cancer comprising inhibition of ATG4B activity in cells; said method comprises administering the subject with an effective amount of tioconazole. The present invention also relates to a method for enhancing or inducing a response in a cancer cell consisting of cytotoxicity, chemosensitivity or starvation-sensitivity; said method comprises administering a subject with an effective amount of tioconazole to inhibit ATG4B's activity.

Abstract: The present invention relates to the area of ATG4-related disorders. More particularly, the present invention relates to a method of treating an ATG4B-related disorder comprising a step of administering a subject with an effective amount of tioconazole. The present invention relates also to a method of increasing a subject's responsiveness to a therapy for a cancer comprising inhibition of ATG4B activity in cells; said method comprises administering the subject with an effective amount of tioconazole. The present invention also relates to a method for enhancing or inducing a response in a cancer cell consisting of cytotoxicity, chemosensitivity or starvation-sensitivity; said method comprises administering a subject with an effective amount of tioconazole to inhibit ATG4B's activity.

Abstract: The present invention provides an evaluating system and the use thereof for the efficacy of antimicrobial peptide, which includes the following steps: (a) constructing a peptide by a first input unit, and load the peptide into an aqueous solution for a first time for equilibration; (b) constructing a lipid bilayer by a second input unit, and load the lipid bilayer into an aqueous solution for a second time for equilibration; (c) Using a first processing unit, simulations are carried out for an aqueous system containing an equilibrated peptide from the first input unit and the equilibrated lipid bilayer from the second input unit; (d) calculating the partition free energy of the peptide by a second processing unit; (e) outputting the prediction by an output unit, wherein the output unit is connected with the first processing unit and the second processing unit.