Abstract: The present disclosure includes provides methods for assessing resting-state fMRI functional connectivity, resting-state MEGI functional connectivity, and/or task-based spatiotemporal auditory cortical activity latency in a subject to detect, monitor, and/or diagnose Tinnitus, with or without hearing impairment. The present disclosure also provides systems, devices, and methods for diagnosing Tinnitus and/or hearing impairment in a subject. Also provided are systems configured for performing the disclosed methods and computer readable medium storing instructions for performing steps of the disclosed methods.