Abstract: A reagent filling device and a sample analyzer with the reagent filling device. The reagent filling device includes a base, a horizontal rotation component, a vertical lifting component and a cantilever component, wherein, the horizontal rotation component is connected with the base, the vertical lifting component moves in a vertical direction relative to the base, the vertical lifting component drives the cantilever component to move along the vertical direction, the cantilever component is rotatably arranged relative to the vertical lifting component, there are a plurality of cantilever components, the cantilever component includes a cantilever body, a length of the cantilever body of at least one cantilever component is greater than a length of the cantilever body of each of the rest cantilever components, and the cantilever component is configured to add a reagent in a reagent disc to a reaction cup in a reaction disc.