Abstract: The present invention provides a non-mediative approach to capture biological cells and initiate cell pairing and fusion in a microfluidic-based system with multiple channels configured in various dimensions and shapes in order to create a hydrodynamic platform incorporating a passive trapping mechanism to entrap the cells of interest from fluid flowing through the multiple channels and allow cell fusion with certain rheological deformation in the absence of any cell disruption while the whole cell pairing and fusion process is closely monitored by simple optical monitoring device.