Abstract: The invention relates to a process for the production of a fructooligosaccharide (FOS) composition on industrial scale, comprising the steps of: a) providing a raw material containing sucrose; b) bringing the raw material together with an enzyme to form a mixture, whereby the enzyme has an endo-inulinase activity and/or a fructosyltransferase activity and is in free, non-immobilized form; c) exposing the mixture to conditions whereby FOS-forming can take place for an amount of time such that the FOS in the mixture constitutes at least 45 wt. % of the total amount of carbohydrates in the mixture, thereby forming the FOS composition; d) optionally deactivating the enzyme; e) recovering the FOS composition. The invention further relates to Fructooligosaccharide composition (FC) wherein: a portion of at most 2 wt. % of the carbohydrates in the FC consists of oligosaccharides that have a degree of polymerisation (DP) of 7 or more; and at least 56 wt.

Abstract: A method is disclosed for producing a transgenic plant with a modified inulin producing profile comprising in its genome a combination of one or more expressible 1-SST enzyme encoding genes and one or more expressible 1-FFT enzyme encoding genes, wherein either of these genes or both of them comprise one or more recombinant genes containing one or more 1-SST, respectively 1-FFT, enzyme encoding DNA sequences of plant origin or an expressible homologous sequence thereof. The invention also relates to a method for modifying and controlling the inulin profile of plants and to a method for producing inulin from said transgenic plants. Furthermore, a novel cDNA sequence of a 1-SST enzyme encoding gene of Helianthus tuberosus and a novel cDNA sequence of a 1-FFT enzyme encoding gene of Cichorium intybus are disclosed, novel recombinant DNA constructs and genes derived thereof, as well as novel combinations of expressible 1-SST and 1-FFT enzyme encoding genes.