Abstract: The present invention relates to methods and tool for amplification and detection of different NRAS isotypes.

Abstract: A method for obtaining a liquid from a porous solid phase is described. The method comprises forming a liquid seal at a first end of a porous solid phase to which a liquid is bound, wherein liquid of the liquid seal is immiscible with the liquid bound to the solid phase, and applying a pressure differential across the porous solid phase to cause the immiscible liquid to move through the porous solid phase towards a second end of the porous solid phase, thereby displacing the liquid bound to the porous solid phase towards the second end and releasing this liquid from the second end. Recovery of liquid from the solid phase using such methods is increased compared with corresponding methods in which no liquid seal is formed. In preferred embodiments, the liquid used to form the liquid seal is a mineral oil. The methods have particular application in nucleic acid extractions which utilise capture of nucleic acid to a solid phase. Kits and apparatus for performing the methods are also described.

Abstract: A method for obtaining a liquid from a porous solid phase is described. The method comprises forming a liquid seal at a first end of a porous solid phase to which a liquid is bound, wherein liquid of the liquid seal is immiscible with the liquid bound to the solid phase, and applying a pressure differential across the porous solid phase to cause the immiscible liquid to move through the porous solid phase towards a second end of the porous solid phase, thereby displacing the liquid bound to the porous solid phase towards the second end and releasing this liquid from the second end. Recovery of liquid from the solid phase using such methods is increased compared with corresponding methods in which no liquid seal is formed. In preferred embodiments, the liquid used to form the liquid seal is a mineral oil. The methods have particular application in nucleic acid extractions which utilize capture of nucleic acid to a solid phase. Kits and apparatus for performing the methods are also described.

Abstract: A method for isolating a nucleic acid comprises: binding the nucleic acid to a solid phase at a first pH in the presence of a binding buffer, washing the bound nucleic acid with a wash solution, and eluting the nucleic acid from the solid phase at a second pH which is higher than the first pH. The wash solution comprises a buffer with a buffering range that encompasses a pH that is higher than the first pH, and the wash solution is at a pH that is within a buffering range of the binding buffer but lower than the buffering range of the buffer of the wash solution. Solutions, compositions, and kits for use in the methods are described.

Abstract: A method for isolating a nucleic acid comprises: binding the nucleic acid to a solid phase at a first pH in the presence of a binding buffer, washing the bound nucleic acid with a wash solution, and eluting the nucleic acid from the solid phase at a second pH which is higher than the first pH. The wash solution comprises a buffer with a buffering range that encompasses a pH that is higher than the first pH, and the wash solution is at a pH that is within a buffering range of the binding buffer but lower than the buffering range of the buffer of the wash solution. Solutions, compositions, and kits for use in the methods are described.

Abstract: In one example, a sealable vent for an ink cartridge or other liquid container includes a recess having a sloping first wall and a removable cover covering the recess and sealing the vent.

Abstract: In one example, a sealable vent for an ink cartridge or other liquid container includes a recess having a sloping first wall and a removable cover covering the recess and sealing the vent.

Abstract: A method for isolating a nucleic acid comprises: binding the nucleic acid to a solid phase at a first pH in the presence of a binding buffer, washing the bound nucleic acid with a wash solution, and eluting the nucleic acid from the solid phase at a second pH which is higher than the first pH. The wash solution comprises a buffer with a buffering range that encompasses a pH that is higher than the first pH, and the wash solution is at a pH that is within a buffering range of the binding buffer but lower than the buffering range of the buffer of the wash solution. Solutions, compositions, and kits for use in the methods are described.

Abstract: Images may be registered using temporal (time-based) and spatial information. In a film implementation, because film is a sequence of frames, using information from neighboring frames may enable a temporally smoother visual experience. In addition, it may be beneficial to take advantage of the fact that consecutive frames are often shifted similarly during the photographic process. Distortion measures may be used that discount candidate transformations that are considered to be too far from one or more preferred transformations, such as, for example, an optimal transformation from another frame or block or a currently-optimal transformation from the same frame/block. Composite color images may be processed to provide registration of underlying components.

Abstract: Images may be registered using temporal (time-based) and spatial information. In a film implementation, because film is a sequence of frames, using information from neighboring frames may enable a temporally smoother visual experience. In addition, it may be beneficial to take advantage of the fact that consecutive frames are often shifted similarly during the photographic process. Distortion measures may be used that discount candidate transformations that are considered to be too far from one or more preferred transformations, such as, for example, an optimal transformation from another frame or block or a currently-optimal transformation from the same frame/block. Composite color images may be processed to provide registration of underlying components.

Abstract: Images may be registered using temporal (time-based) and spatial information. In a film implementation, because film is a sequence of frames, using information from neighboring frames may enable a temporally smoother visual experience. In addition, it may be beneficial to take advantage of the fact that consecutive frames are often shifted similarly during the photographic process. Distortion measures may be used that discount candidate transformations that are considered to be too far from one or more preferred transformations, such as, for example, an optimal transformation from another frame or block or a currently-optimal transformation from the same frame/block. Composite color images may be processed to provide registration of underlying components.

Abstract: Images may be registered using temporal (time-based) and spatial information. In a film implementation, because film is a sequence of frames, using information from neighboring frames may enable a temporally smoother visual experience. In addition, it may be beneficial to take advantage of the fact that consecutive frames are often shifted similarly during the photographic process. Distortion measures may be used that discount candidate transformations that are considered to be too far from one or more preferred transformations, such as, for example, an optimal transformation from another frame or block or a currently-optimal transformation from the same frame/block. Composite color images may be processed to provide registration of underlying components.

Abstract: Images may be registered using temporal (time-based) and spatial information. In a film implementation, because film is a sequence of frames, using information from neighboring frames may enable a temporally smoother visual experience. In addition, it may be beneficial to take advantage of the fact that consecutive frames are often shifted similarly during the photographic process. Distortion measures may be used that discount candidate transformations that are considered to be too far from one or more preferred transformations, such as, for example, an optimal transformation from another frame or block or a currently-optimal transformation from the same frame/block. Composite color images may be processed to provide registration of underlying components.

Abstract: Images may be registered using temporal (time-based) and spatial information. In a film implementation, because film is a sequence of frames, using information from neighboring frames may enable a temporally smoother visual experience. In addition, it may be beneficial to take advantage of the fact that consecutive frames are often shifted similarly during the photographic process. Distortion measures may be used that discount candidate transformations that are considered to be too far from one or more preferred transformations, such as, for example, an optimal transformation from another frame or block or a currently-optimal transformation from the same frame/block. Composite color images may be processed to provide registration of underlying components.