Abstract: The present disclosure relates to the technical fields of agriculture and environmental microbiology, and provides Agrobacterium for reducing uptake of heavy metals by wheat. The Agrobacterium is strain NJ20, which is deposited in China General Microbiological Culture Collection Center under Accession CGMCC No. 26197. The present disclosure also provides a bacterial agent which is prepared by the aforementioned Agrobacterium. The Agrobacterium and bacterial agent thereof are used for reducing uptake of heavy metals in heavy metal contaminated soil by wheat. The strain NJ20 of the present disclosure can grow well under the stress of lead and cadmium, and remove soluble lead and cadmium in the culture solution by adsorption; meanwhile the strain NJ20 can generate indoleacetic acid, dissolve insoluble tricalcium phosphate and improve phosphorus nutrition in soil. Inoculation of the strain NJ20 into heavy metal contaminated soil can reduce the contents of lead and cadmium in wheat grains and straws.