Patents by Inventor Lennart Johansson
Lennart Johansson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8895710Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.Type: GrantFiled: January 4, 2011Date of Patent: November 25, 2014Assignee: GE Healthcare Bio-Sciences ABInventors: Carina Engstrand, Annika Forss, Gunnar Glad, Bo-Lennart Johansson, Hans J. Johansson, Jean-Luc Maloisel
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Publication number: 20140243498Abstract: The present invention is within the field of biomolecule purification. More closely the invention relates to chromatographic purification of insulin using a specific kind of shell beads having an inner core and an outer functionalized layer. The method enables purification at high flow rates and high purity, over 90%.Type: ApplicationFiled: September 28, 2012Publication date: August 28, 2014Inventors: Eggert Brekkan, Kjell Eriksson, Bo-Lennart Johansson, Jamil Shanagar
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Patent number: 8685248Abstract: The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilized, wherein said ligands comprise at least one aliphatic sulphonamide. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to a separation matrix that comprises aliphatic sulphonamide ligands.Type: GrantFiled: February 22, 2010Date of Patent: April 1, 2014Assignee: GE Healthcare Bio-Sciences ABInventors: Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel, Nils Norrman
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Publication number: 20140069329Abstract: A gluing unit for applying adhesive to a succession of opening devices to be fitted to respective sealed packages of food products pourable into a tube of packaging material, the unit having a conveyor for feeding the opening devices along a path, and an adhesive dispenser located along the path, interacting with each opening device to apply adhesive to a portion of the opening device, and movable in a plane parallel to the path as well as crosswise to the plane.Type: ApplicationFiled: November 18, 2013Publication date: March 13, 2014Applicant: TETRA LAVAL HOLDINGS & FINANCE S.A.Inventors: Andrea MACCAGNANI, Lars Skarin, Lennart Johansson, Mahmod Tabarte
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Publication number: 20140017813Abstract: The present invention relates to a method for depletion of undesired molecules and/or enrichment of desired molecules from a sample comprising high abundant as well as low abundant molecules, comprising the following steps: a) providing a separation material comprising a solid phase (beads) comprising an inner porous core material comprising magnetic particles and an outer porous shell with a porosity equal or denser than that of the shell; b) adding the sample to the separation material; c) adsorbing a first fraction of molecules with a molecular weight of 500-50 000 Da in the core and simultaneously excluding a second fraction of molecules from binding to the core and the shell, wherein the molecular weight of the second fraction molecules is at least 5 preferably 10 times higher than the molecular weight of the first fraction and d) eluting the desired molecules from the separation material, wherein step d) and optionally step c) is performed using an oscillating power/field applied over the separation matType: ApplicationFiled: March 29, 2012Publication date: January 16, 2014Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel
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Patent number: 8584614Abstract: A gluing unit for applying adhesive to a succession of opening devices to be fitted to respective sealed packages of food products pourable into a tube of packaging material, the unit having a conveyor for feeding the opening devices along a path, and an adhesive dispenser located along the path, interacting with each opening device to apply adhesive to a portion of the opening device, and movable in a plane parallel to the path as well as crosswise to the plane.Type: GrantFiled: October 22, 2009Date of Patent: November 19, 2013Assignee: Tetra Laval Holdings & Finance S.A.Inventors: Andrea MacCagnani, Lars Skarin, Lennart Johansson, Mahmod Tabarte
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Patent number: 8574437Abstract: The present invention relates to a method of producing novel chromatography media and use thereof for purification of biomolecules, such as proteins. The chromatography media comprises shell beads having an inner porous core and an outer shell. The method comprises providing buffering ligands in the core of the beads, and providing binding ligands aimed for biomolecule binding in the outer shell of the beads. This method makes it possible to optimize binding properties and buffering properties independently of each other which is especially to advantageous for production of chromatofocusing media.Type: GrantFiled: February 16, 2011Date of Patent: November 5, 2013Assignee: GE Healthcare Bio-Sciences ABInventors: Jan Bergstrom, Bo-Lennart Johansson
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Publication number: 20130244229Abstract: The present invention relates to a buffering composition, more closely a composition comprising porous matrix-enclosed buffering agent(s) giving a stabilisation of pH when applied in for example aqueous environment. The composition comprises buffering agent(s) enclosed in a first porous matrix with an impenetrability corresponding to a fractionation range for globular proteins and peptides of <10000 g/mol, preferably <5000 g/mol, more preferably <700 g/mol.Type: ApplicationFiled: November 29, 2011Publication date: September 19, 2013Inventors: Jan Bergstrom, Bo-Lennart Johansson
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Publication number: 20130115709Abstract: The present invention relates to a method for running ion exchange chromatography on a media comprising shell beads having an inner porous core and an outer shell, wherein the inner core is provided with ligands whose charge changes with pH and the shell is provided with charged ion exchange ligands, the method comprising the following steps: a) adsorbing sample molecules on the shell ligands at a first pH; b) causing a discharge of the inner core ligands at a second pH by addition of a buffer substance that is able to increase its charge having the same sign/type as that of the core ligands, which at the same time causes release of ions from the inner core ligands and thereby an increase in ionic strength that displaces the sample molecules from the shell ligands i.e. causes an elution.Type: ApplicationFiled: June 28, 2011Publication date: May 9, 2013Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Jan Bergstrom, Bo-Lennart Johansson
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Publication number: 20130040809Abstract: The present invention relates to a method and means to produce chromatography media having improved pressure-flow properties. More closely, the invention relates to bimodal particle size distribution and the use of layer functionalisation as means to change pressure-flow properties of chromatography media. The invention relates to a method for production of chromatography media having improved pressure-flow properties, comprising mixing large beads/particles, comprising an inner core and an outer functionalized shell/lid, with smaller beads/particles, wherein the ratio of the particle size of large and small beads: [D50V for large particles/D50V for small particles]>1.2, and wherein the volume ratio of large and small beads in the column: [Total volume of large beds/Total volume beads] is in the range 0.05-0.9.Type: ApplicationFiled: April 20, 2011Publication date: February 14, 2013Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Jan Bergstrom, Bo-Lennart Johansson
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Patent number: 8372286Abstract: The present invention relates to a method for production of separation media using a so called Spinning Disc technology wherein the porosities of the beads are optimized in such a way that a desired biomolecule may be separated from a complex sample. The method comprises the following steps: a) feeding a 4-8% polysaccharide solution, which has a viscosity within 350-450 mPas, at 65-75° C. to one or more spinning discs at 3001-3010 rpm to form polysaccharide beads; b) capturing said formed polysaccharide beads in a capturing bath; wherein the porosity of the polysaccharide beads is controlled by varying the temperature of the capturing between 15 and 27° C., preferably between 17.5 and 24.6° C. The method yields porosities that prevent molecules larger than 150 000 g/mol to diffuse into the beads. The invention also relates to separation media produced by the method and use thereof for purification of biomolecules, in particular monoclonal antibodies.Type: GrantFiled: January 30, 2009Date of Patent: February 12, 2013Assignee: GE Healthcare Bio-Sciences ABInventors: Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel, Nils Norrman, Ake Stenholm
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Publication number: 20120309618Abstract: The present invention relates to a method of producing novel chromatography media and use thereof for purification of biomolecules, such as proteins. The chromatography media comprises shell beads having an inner porous core and an outer shell. The method comprises providing buffering ligands in the core of the beads, and providing binding ligands aimed for biomolecule binding in the outer shell of the beads. This method makes it possible to optimize binding properties and buffering properties independently of each other which is especially to advantageous for production of chromatofocusing media.Type: ApplicationFiled: February 16, 2011Publication date: December 6, 2012Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Jan Bergstrom, Bo-Lennart Johansson
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Patent number: 8114280Abstract: The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilized, wherein said ligands comprise at least one sulphonamide and the R group of the sulphonyl comprises an aromatic group. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to the separation matrix.Type: GrantFiled: June 22, 2005Date of Patent: February 14, 2012Assignee: GE Healthcare Bio-Sciences ABInventors: Jean-Luc Maloisel, Bo-Lennart Johansson, Gunnar Glad, Nils Norrman
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Patent number: 8092683Abstract: The present invention relates to a method of preparing a library of resins which are useful in chromatography, which method comprises creating a diversity of multi-modal ion exchange resins; and providing the diversity in a parallel system in which each resin is presented separated from the other resin(s).Type: GrantFiled: January 10, 2008Date of Patent: January 10, 2012Assignee: GE Healthcare Bio-Sciences ABInventors: Enrique Carredano, Bo-Lennart Johansson, Jean-Luc Maloisel
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Patent number: 8070958Abstract: The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.Type: GrantFiled: January 25, 2010Date of Patent: December 6, 2011Assignee: GE Healthcare Bio-Sciences ABInventors: Jan Bergstrom, Gunnar Glad, Bo-Lennart Johansson, Anders Ljunglof, Jean-Luc Maloisel
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Publication number: 20110266225Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.Type: ApplicationFiled: June 13, 2011Publication date: November 3, 2011Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: BO-LENNART JOHANSSON, JEAN-LUC MALOISEL, HANS J. JOHANSSON, ASA EK, MARIA HOLM, JAFAR SAIDZADEH
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Publication number: 20110174216Abstract: A gluing unit for applying adhesive to a succession of opening devices to be fitted to respective sealed packages of food products pourable into a tube of packaging material, the unit having a conveyor for feeding the opening devices along a path, and an adhesive dispenser located along the path, interacting with each opening device to apply adhesive to a portion of the opening device, and movable in a plane parallel to the path as well as crosswise to the plane.Type: ApplicationFiled: October 22, 2009Publication date: July 21, 2011Applicant: TETRA LAVAL HOLDINGS & FINANCE S.A.Inventors: Andrea Maccagnani, Lars Skarin, Lennart Johansson, Mahmod Tabarte
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Publication number: 20110155668Abstract: The present invention relates to a separation medium, comprising an inner core of a porous material provided with charged ligands, and an outer lid comprising a porous material provided with charged ligands, wherein the charge of the ligands in the inner core is opposite that of the charge of the ligands in the lid. The present invention also relates to a method for biomolecule separation comprising applying a sample to the above separation medium, wherein large molecules are prevented from entering the medium by charge repulsion from the medium and small molecules are captured in the inner core.Type: ApplicationFiled: June 4, 2009Publication date: June 30, 2011Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel, Nils Norrman
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Publication number: 20110118442Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.Type: ApplicationFiled: January 4, 2011Publication date: May 19, 2011Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: CARINA ENGSTRAND, Annika Forss, Gunnar Glad, Bo-Lennart Johansson, Hans J. Johansson, Jean-Luc Maloisel
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Publication number: 20110065900Abstract: The present invention relates to a method for removing at least one negatively charged substance from an aqueous liquid by contacting the liquid with a separation matrix comprising a plurality of polyallylamine ligands, comprising binding said negatively charged substance to said ligands under conditions where the ionic strength of the aqueous liquid applied to the chromatography resin ?0.25 M NaCl.Type: ApplicationFiled: May 29, 2009Publication date: March 17, 2011Applicant: GE HEALTHCARE BIO-SCIENCE ABInventors: Bo-Lennart Johansson, Ulrika Karlsson, Jean-Luc Maloisel