Abstract: A method of obtaining a non-human mammal susceptible to adenovirus-mediated gene delivery, a method for such delivery, and a transgenic non-human mammal susceptible to adenovirus-mediated gene delivery, and more specifically a trans-genic mouse that expresses a cytoplasmically truncated human Coxsackievirus and Adenovirus Receptor (hCAR) in essentially all tissues thereof. The mammal allows for efficient infections at low multiplicity of infection (MOI) into cells that are normally resistant or not very susceptible to adenovirus-mediated gene delivery, such as spleenocytes and dendritic cells (DC). The hCAR transgenic mammal is highly susceptible to adenovirus-mediated gene transfer and will be a useful tool to probe gene function in development and to elucidate molecular pathways, dynamic properties and differentiation mechanisms in non-transformed cells.