Abstract: The present disclosure includes MGMT modifications that cause MGMT resistance to MGMT inhibitors. The present disclosure also includes in vivo, in vitro, and ex vivo modification of MGMT-encoding nucleic acids to encode and express an MGMT variant that is resistant to MGMT inhibitors. Inhibitor-resistant MGMT modifications and modification of an MGMT-encoding nucleic can occur or be used in conjunction with a therapeutic modification, e.g., in a single cell. Expression of inhibitor-resistant MGMT can selectively protect modified cells from a selection regimen, such as a selection regimen including an MGMT inhibitor and an alkylating agent. Accordingly, in vivo, in vitro, and ex vivo modification of MGMT-encoding nucleic acids can be used in gene therapy to select for modified cells.

Abstract: A system and method are provided for monitoring utilization of network resources in a photonic network. A monitoring threshold is set for monitoring utilization of network components of interest. A resource utilization data processor obtains resource utilization data indicating utilization of the network resources. A threshold crossing determination unit determines if threshold crossing has occurred based on the utilization data and the monitoring threshold. A user interface indicates the utilization of the network components in accordance with the determination of the threshold crossing.

Abstract: In a method of determining the availability of a network resource in a digital communications network, atomic resources that are not composed of other resources are first identified. A degradation factor is assigned to each atomic resource. Subsequently a degradation factor for a composite resource is derived from the degradation factors of the atomic resources in accordance with a predetermined relationship.