Abstract: A micro IVF chamber that enables rapid filling, hermetic sealing, and custom gas environment control for secure cell extraction, fertilization, culturing, insemination, macro and microscopic content examination, post examination rapid refilling, return to optimal gas levels and pH values, with direct view of cell in conventional microscopes while maintaining culture environment, designed for use in any incubator and multiple devices per shelf for optimum efficiency while maintaining integrity of individual devices and patient-culture identity in a simple one-sample clamp-and-go device is disclosed.