Patents by Inventor Leon Meadows

Leon Meadows has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10988513
    Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.
    Type: Grant
    Filed: June 24, 2016
    Date of Patent: April 27, 2021
    Assignees: AMYRIS, INC., TOTAL MARKETING SERVICES
    Inventors: Penelope R. Chua, Hanxiao Jiang, Adam Leon Meadows
  • Patent number: 10808015
    Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.
    Type: Grant
    Filed: June 24, 2016
    Date of Patent: October 20, 2020
    Assignee: AMYRIS, INC.
    Inventors: Penelope R. Chua, Hanxiao Jiang, Adam Leon Meadows
  • Patent number: 9914941
    Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.
    Type: Grant
    Filed: September 2, 2014
    Date of Patent: March 13, 2018
    Assignee: AMYRIS, INC.
    Inventors: Timothy Stevens Gardner, Kristy Michelle Hawkins, Adam Leon Meadows, Annie Ening Tsong, Yoseph Tsegaye
  • Patent number: 9500573
    Abstract: Systems and methods provide for testing a cement by applying force in a first direction until the first portion of the cement sample fails in compression or tension; and applying force a second direction opposite the first direction until the second portion of the cement sample fails in tension or compression.
    Type: Grant
    Filed: July 21, 2014
    Date of Patent: November 22, 2016
    Assignee: Halliburton Energy Services, Inc.
    Inventors: David Leon Meadows, Robert Phillip Darbe, Walmy Cuello Jimenez, Robert Lee Browning
  • Patent number: 9410214
    Abstract: Provided herein are compositions and methods for improved production of acetyl-CoA and acetyl-CoA derived compounds in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a phosphoketolase (PK), and a functional disruption of an endogenous enzyme that converts acetyl phosphate to acetate. In some embodiments, the host cell further comprises a heterologous nucleotide sequence encoding a phosphotransacetylase (PTA). In some embodiments, the enzyme that converts acetyl phosphate to acetate is a glycerol-1-phosphatase. In some embodiments, the glycerol-1-phosphatase is GPP1/RHR2. In some embodiments, the glycerol-1-phosphatase is GPP2/HOR2. The compositions and methods described herein provide an efficient route for the heterologous production of acetyl-CoA-derived compounds, including but not limited to, isoprenoids, polyketides, and fatty acids.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: August 9, 2016
    Assignee: Amyris, Inc.
    Inventors: Kristy Michelle Hawkins, Tina Tipawan Mahatdejkul-Meadows, Adam Leon Meadows, Lauren Barbara Pickens, Anna Tai, Annie Ening Tsong
  • Patent number: 9151154
    Abstract: An apparatus for preparing and testing a sample of a subterranean formation, the apparatus comprising a pressure cell defining an interior volume, the pressure cell comprising a first end member comprising a channel formed therein, a second end member, a wall member positioned between the first end member and the second end member, and a sample cell positioned within the interior volume of the pressure cell, wherein the channel of the first end member fluidly connects with a first point external of the pressure cell, with a second point external of the pressure cell, and with the sample cell.
    Type: Grant
    Filed: May 31, 2013
    Date of Patent: October 6, 2015
    Assignee: Halliburton Energy Services, Inc.
    Inventor: David Leon Meadows
  • Patent number: 8960013
    Abstract: Systems and methods provide for testing a cement by applying force in a first direction until the first portion of the cement sample fails in compression or tension; and applying force a second direction opposite the first direction until the second portion of the cement sample fails in tension or compression.
    Type: Grant
    Filed: March 1, 2012
    Date of Patent: February 24, 2015
    Assignee: Halliburton Energy Services, Inc.
    Inventors: David Leon Meadows, Robert Phillip Darbe, Walmy Cuello Jimenez, Robert Lee Browning
  • Publication number: 20140326073
    Abstract: Systems and methods provide for testing a cement by applying force in a first direction until the first portion of the cement sample fails in compression or tension; and applying force a second direction opposite the first direction until the second portion of the cement sample fails in tension or compression.
    Type: Application
    Filed: July 21, 2014
    Publication date: November 6, 2014
    Inventors: David Leon Meadows, Robert Phillip Darbe, Walmy Cuello Jimenez, Robert Lee Browning
  • Patent number: 8859261
    Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.
    Type: Grant
    Filed: October 24, 2013
    Date of Patent: October 14, 2014
    Assignee: Amyris, Inc.
    Inventors: Timothy Stevens Gardner, Kristy Michelle Hawkins, Adam Leon Meadows, Annie Ening Tsong, Yoseph Tsegaye
  • Publication number: 20140273144
    Abstract: Provided herein are compositions and methods for improved production of acetyl-CoA and acetyl-CoA derived compounds in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a phosphoketolase (PK), and a functional disruption of an endogenous enzyme that converts acetyl phosphate to acetate. In some embodiments, the host cell further comprises a heterologous nucleotide sequence encoding a phosphotransacetylase (PTA). In some embodiments, the enzyme that converts acetyl phosphate to acetate is a glycerol-1-phosphatase. In some embodiments, the glycerol-1-phosphatase is GPP1/RHR2. In some embodiments, the glycerol-1-phosphatase is GPP2/HOR2. The compositions and methods described herein provide an efficient route for the heterologous production of acetyl-CoA-derived compounds, including but not limited to, isoprenoids, polyketides, and fatty acids.
    Type: Application
    Filed: March 14, 2014
    Publication date: September 18, 2014
    Inventors: Kristy Michelle Hawkins, Tina Tipawan Mahatdejkul-Meadows, Adam Leon Meadows, Lauren Barbara Pickens, Anna Tai, Annie Ening Tsong
  • Patent number: 8794078
    Abstract: Systems and methods provide for testing a cement by measuring both axial shrinkage/expansion and radial shrinkage/expansion of a sample.
    Type: Grant
    Filed: July 5, 2012
    Date of Patent: August 5, 2014
    Assignee: Halliburton Energy Services, Inc.
    Inventors: Robert Phillip Darbe, David Leon Meadows, Walmy Cuello Jimenez
  • Publication number: 20140154765
    Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.
    Type: Application
    Filed: October 24, 2013
    Publication date: June 5, 2014
    Applicant: AMYRIS, INC.
    Inventors: Timothy Stevens GARDNER, Kristy Michelle Hawkins, Adam Leon Meadows, Annie Ening Tsong, Yoseph Tsegaye
  • Publication number: 20140007695
    Abstract: Systems and methods provide for testing a cement by measuring both axial shrinkage/expansion and radial shrinkage/expansion of a sample.
    Type: Application
    Filed: July 5, 2012
    Publication date: January 9, 2014
    Applicant: HALLIBURTON ENERGY SERVICES, INC.
    Inventors: Robert Phillip Darbe, David Leon Meadows, Walmy Cuello Jimenez
  • Patent number: 8603800
    Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.
    Type: Grant
    Filed: January 28, 2013
    Date of Patent: December 10, 2013
    Assignee: AMYRIS, Inc.
    Inventors: Timothy Stevens Gardner, Kristy Michelle Hawkins, Adam Leon Meadows, Annie Ening Tsong, Yoseph Tsegaye
  • Publication number: 20130228019
    Abstract: Systems and methods provide for testing a cement by applying force in a first direction until the first portion of the cement sample fails in compression or tension; and applying force a second direction opposite the first direction until the second portion of the cement sample fails in tension or compression.
    Type: Application
    Filed: March 1, 2012
    Publication date: September 5, 2013
    Inventors: David Leon Meadows, Robert Phillip Darbe, Walmy Cuello Jimenez, Robert Lee Browning
  • Patent number: 8415136
    Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.
    Type: Grant
    Filed: November 9, 2012
    Date of Patent: April 9, 2013
    Assignee: Amyris, Inc.
    Inventors: Timothy Stevens Gardner, Kristy Michelle Hawkins, Adam Leon Meadows, Annie Ening Tsong, Yoseph Tsegaye
  • Patent number: 5775714
    Abstract: A tow bar (10) is disclosed which has first and second arms (34, 40) mounted on a member (16) on the towing vehicle (12). The arms can vary in length between a shortened storage configuration, where they are secured to the first member, to a lengthened towing configuration where they are attached to a second member (70) attached to the towed vehicle (14). The arms are attached to the members for pivotal motion about vertical axes. The arms themselves have horizontal pivot hinges near each end thereof and a hinge permitting each arm to twist. The tow bar provides for efficient backing.
    Type: Grant
    Filed: March 15, 1996
    Date of Patent: July 7, 1998
    Inventor: Leon Meadows
  • Patent number: 4756390
    Abstract: An improved remote braking system (10, 100) is provided which allows simultaneous actuation of the braking system of a motor home (12) and an automobile (14) towed behind the motor home. An advantage of the present invention is the incorporation of the system within the self-contained hydraulic brake system of the automobile, which permits the braking system of the automobile to be operated in the normal manner. The system includes an actuator (26, 102) which is secured between the vacuum booster (18) and the master cylinder (20) of the automobile. Within the actuator is a piston (38, 114) which separates the interior of the actuator into two chambers. A pressure differential is created between the chambers when the brakes of the motor home are applied to generate a force on the piston (38, 114), to apply the brakes of the towed automobile as well. The system is operable with either air brakes or hydraulic brakes in the motor home.
    Type: Grant
    Filed: July 15, 1987
    Date of Patent: July 12, 1988
    Inventor: Leon Meadows