Patents by Inventor Leon Meadows
Leon Meadows has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10988513Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.Type: GrantFiled: June 24, 2016Date of Patent: April 27, 2021Assignees: AMYRIS, INC., TOTAL MARKETING SERVICESInventors: Penelope R. Chua, Hanxiao Jiang, Adam Leon Meadows
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Patent number: 10808015Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.Type: GrantFiled: June 24, 2016Date of Patent: October 20, 2020Assignee: AMYRIS, INC.Inventors: Penelope R. Chua, Hanxiao Jiang, Adam Leon Meadows
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Patent number: 9914941Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.Type: GrantFiled: September 2, 2014Date of Patent: March 13, 2018Assignee: AMYRIS, INC.Inventors: Timothy Stevens Gardner, Kristy Michelle Hawkins, Adam Leon Meadows, Annie Ening Tsong, Yoseph Tsegaye
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Patent number: 9500573Abstract: Systems and methods provide for testing a cement by applying force in a first direction until the first portion of the cement sample fails in compression or tension; and applying force a second direction opposite the first direction until the second portion of the cement sample fails in tension or compression.Type: GrantFiled: July 21, 2014Date of Patent: November 22, 2016Assignee: Halliburton Energy Services, Inc.Inventors: David Leon Meadows, Robert Phillip Darbe, Walmy Cuello Jimenez, Robert Lee Browning
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Patent number: 9410214Abstract: Provided herein are compositions and methods for improved production of acetyl-CoA and acetyl-CoA derived compounds in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a phosphoketolase (PK), and a functional disruption of an endogenous enzyme that converts acetyl phosphate to acetate. In some embodiments, the host cell further comprises a heterologous nucleotide sequence encoding a phosphotransacetylase (PTA). In some embodiments, the enzyme that converts acetyl phosphate to acetate is a glycerol-1-phosphatase. In some embodiments, the glycerol-1-phosphatase is GPP1/RHR2. In some embodiments, the glycerol-1-phosphatase is GPP2/HOR2. The compositions and methods described herein provide an efficient route for the heterologous production of acetyl-CoA-derived compounds, including but not limited to, isoprenoids, polyketides, and fatty acids.Type: GrantFiled: March 14, 2014Date of Patent: August 9, 2016Assignee: Amyris, Inc.Inventors: Kristy Michelle Hawkins, Tina Tipawan Mahatdejkul-Meadows, Adam Leon Meadows, Lauren Barbara Pickens, Anna Tai, Annie Ening Tsong
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Patent number: 9151154Abstract: An apparatus for preparing and testing a sample of a subterranean formation, the apparatus comprising a pressure cell defining an interior volume, the pressure cell comprising a first end member comprising a channel formed therein, a second end member, a wall member positioned between the first end member and the second end member, and a sample cell positioned within the interior volume of the pressure cell, wherein the channel of the first end member fluidly connects with a first point external of the pressure cell, with a second point external of the pressure cell, and with the sample cell.Type: GrantFiled: May 31, 2013Date of Patent: October 6, 2015Assignee: Halliburton Energy Services, Inc.Inventor: David Leon Meadows
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Patent number: 8960013Abstract: Systems and methods provide for testing a cement by applying force in a first direction until the first portion of the cement sample fails in compression or tension; and applying force a second direction opposite the first direction until the second portion of the cement sample fails in tension or compression.Type: GrantFiled: March 1, 2012Date of Patent: February 24, 2015Assignee: Halliburton Energy Services, Inc.Inventors: David Leon Meadows, Robert Phillip Darbe, Walmy Cuello Jimenez, Robert Lee Browning
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Publication number: 20140326073Abstract: Systems and methods provide for testing a cement by applying force in a first direction until the first portion of the cement sample fails in compression or tension; and applying force a second direction opposite the first direction until the second portion of the cement sample fails in tension or compression.Type: ApplicationFiled: July 21, 2014Publication date: November 6, 2014Inventors: David Leon Meadows, Robert Phillip Darbe, Walmy Cuello Jimenez, Robert Lee Browning
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Patent number: 8859261Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.Type: GrantFiled: October 24, 2013Date of Patent: October 14, 2014Assignee: Amyris, Inc.Inventors: Timothy Stevens Gardner, Kristy Michelle Hawkins, Adam Leon Meadows, Annie Ening Tsong, Yoseph Tsegaye
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Publication number: 20140273144Abstract: Provided herein are compositions and methods for improved production of acetyl-CoA and acetyl-CoA derived compounds in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a phosphoketolase (PK), and a functional disruption of an endogenous enzyme that converts acetyl phosphate to acetate. In some embodiments, the host cell further comprises a heterologous nucleotide sequence encoding a phosphotransacetylase (PTA). In some embodiments, the enzyme that converts acetyl phosphate to acetate is a glycerol-1-phosphatase. In some embodiments, the glycerol-1-phosphatase is GPP1/RHR2. In some embodiments, the glycerol-1-phosphatase is GPP2/HOR2. The compositions and methods described herein provide an efficient route for the heterologous production of acetyl-CoA-derived compounds, including but not limited to, isoprenoids, polyketides, and fatty acids.Type: ApplicationFiled: March 14, 2014Publication date: September 18, 2014Inventors: Kristy Michelle Hawkins, Tina Tipawan Mahatdejkul-Meadows, Adam Leon Meadows, Lauren Barbara Pickens, Anna Tai, Annie Ening Tsong
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Patent number: 8794078Abstract: Systems and methods provide for testing a cement by measuring both axial shrinkage/expansion and radial shrinkage/expansion of a sample.Type: GrantFiled: July 5, 2012Date of Patent: August 5, 2014Assignee: Halliburton Energy Services, Inc.Inventors: Robert Phillip Darbe, David Leon Meadows, Walmy Cuello Jimenez
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Publication number: 20140154765Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.Type: ApplicationFiled: October 24, 2013Publication date: June 5, 2014Applicant: AMYRIS, INC.Inventors: Timothy Stevens GARDNER, Kristy Michelle Hawkins, Adam Leon Meadows, Annie Ening Tsong, Yoseph Tsegaye
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Publication number: 20140007695Abstract: Systems and methods provide for testing a cement by measuring both axial shrinkage/expansion and radial shrinkage/expansion of a sample.Type: ApplicationFiled: July 5, 2012Publication date: January 9, 2014Applicant: HALLIBURTON ENERGY SERVICES, INC.Inventors: Robert Phillip Darbe, David Leon Meadows, Walmy Cuello Jimenez
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Patent number: 8603800Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.Type: GrantFiled: January 28, 2013Date of Patent: December 10, 2013Assignee: AMYRIS, Inc.Inventors: Timothy Stevens Gardner, Kristy Michelle Hawkins, Adam Leon Meadows, Annie Ening Tsong, Yoseph Tsegaye
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Publication number: 20130228019Abstract: Systems and methods provide for testing a cement by applying force in a first direction until the first portion of the cement sample fails in compression or tension; and applying force a second direction opposite the first direction until the second portion of the cement sample fails in tension or compression.Type: ApplicationFiled: March 1, 2012Publication date: September 5, 2013Inventors: David Leon Meadows, Robert Phillip Darbe, Walmy Cuello Jimenez, Robert Lee Browning
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Patent number: 8415136Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.Type: GrantFiled: November 9, 2012Date of Patent: April 9, 2013Assignee: Amyris, Inc.Inventors: Timothy Stevens Gardner, Kristy Michelle Hawkins, Adam Leon Meadows, Annie Ening Tsong, Yoseph Tsegaye
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Patent number: 5775714Abstract: A tow bar (10) is disclosed which has first and second arms (34, 40) mounted on a member (16) on the towing vehicle (12). The arms can vary in length between a shortened storage configuration, where they are secured to the first member, to a lengthened towing configuration where they are attached to a second member (70) attached to the towed vehicle (14). The arms are attached to the members for pivotal motion about vertical axes. The arms themselves have horizontal pivot hinges near each end thereof and a hinge permitting each arm to twist. The tow bar provides for efficient backing.Type: GrantFiled: March 15, 1996Date of Patent: July 7, 1998Inventor: Leon Meadows
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Patent number: 4756390Abstract: An improved remote braking system (10, 100) is provided which allows simultaneous actuation of the braking system of a motor home (12) and an automobile (14) towed behind the motor home. An advantage of the present invention is the incorporation of the system within the self-contained hydraulic brake system of the automobile, which permits the braking system of the automobile to be operated in the normal manner. The system includes an actuator (26, 102) which is secured between the vacuum booster (18) and the master cylinder (20) of the automobile. Within the actuator is a piston (38, 114) which separates the interior of the actuator into two chambers. A pressure differential is created between the chambers when the brakes of the motor home are applied to generate a force on the piston (38, 114), to apply the brakes of the towed automobile as well. The system is operable with either air brakes or hydraulic brakes in the motor home.Type: GrantFiled: July 15, 1987Date of Patent: July 12, 1988Inventor: Leon Meadows