Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods of storing columns in a wet state.

Abstract: A novel class of capillary electrophoresis probes and a method of employing the same are described. The novel class of capillary electrophoresis probes are comprised of one or more vinylogous carboxylic acid compounds or their derivatives. In another aspect of the present invention, a method of detecting ions in water by capillary electrophoresis is provided wherein: water is introduced into a capillary filled with an electrolyte which contains UV absorbing species comprised of one or more vinylogous carboxylic acid compounds. An electric voltage is applied along the capillary to cause ions present in the water to move along the capillary and separate. The ions displace the UV absorbing species and are detected indirectly by a UV/visible light photometric detector.

Abstract: The subject invention provides extraction capillaries, wherein a substantial portion of the channel is coated with a 3-dimensional solid phase extraction surface that binds an analyte. In some embodiments the extraction matrix comprises a polymer backbone with an extraction agent bound thereto. Analytes of particular relevance include biomolecules, such as proteins, polynucleotides, lipids and polysaccharides. The invention further provides devices comprising the extraction capillaries, reagents for use in conjunction with the capillaries and devices, and methods for the production and use of the capillaries and devices.

Abstract: A novel class of capillary electrophoresis probes and a method of employing the same are described. The novel class of capillary electrophoresis probes are comprised of one or more vinylogous carboxylic acid compounds or their derivatives. In another aspect of the present invention, a method of detecting ions in water by capillary electrophoresis is provided wherein: water is introduced into a capillary filled with an electrolyte which contains UV absorbing species comprised of one or more vinylogous carboxylic acid compounds. An electric voltage is applied along the capillary to cause ions present in the water to move along the capillary and separate. The ions displace the UV absorbing species and are detected indirectly by a UV/visible light photometric detector.