Patents by Inventor Leonard J. Di Sera

Leonard J. Di Sera has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • AUTOMATED HYBRIDIZATION/IMAGING DEVICE FOR FLUORESCENT MULTIPLEX DNA SEQUENCING
    Publication number: 20020012910
    Abstract: A method is disclosed for automated multiplex sequencing of DNA with an integrated automated imaging hybridization chamber system. This system comprises an hybridization chamber device for mounting a membrane containing size-fractionated multiplex sequencing reaction products, apparatus for fluid delivery to the chamber device, imaging apparatus for light delivery to the membrane and image recording of fluorescence emanating from the membrane while in the chamber device, and programmable controller apparatus for controlling operation of the system. The multiplex reaction products are hybridized with a probe, then an enzyme (such as alkaline phosphatase) is bound to a binding moiety on the probe, and a fluorogenic substrate (such as a benzothiazole derivative) is introduced into the chamber device by the fluid delivery apparatus.
    Type: Application
    Filed: November 28, 1995
    Publication date: January 31, 2002
    Inventors: ROBERT B. WEISS, ALVIN W. KIMBALL, RAYMOND F. GESTELAND, F. MARK FERGUSON, DIANE M. DUNN, LEONARD J. DI SERA, JOSHUA L. CHERRY
  • Automated hybridization/imaging device for fluorescent multiplex DNA sequencing
    Patent number: 5470710
    Abstract: A method is disclosed for automated multiplex sequencing of DNA with an integrated automated imaging hybridization chamber system. This system comprises an hybridization chamber device for mounting a membrane containing size-fractionated multiplex sequencing reaction products, apparatus for fluid delivery to the chamber device, imaging apparatus for light delivery to the membrane and image recording of fluorescence emanating from the membrane while in the chamber device, and programmable controller apparatus for controlling operation of the system. The multiplex reaction products are hybridized with a probe, then an enzyme (such as alkaline phosphatase) is bound to a binding moiety on the probe, and a fluorogenic substrate (such as a benzothiazole derivative) is introduced into the chamber device by the fluid delivery apparatus.
    Type: Grant
    Filed: October 22, 1993
    Date of Patent: November 28, 1995
    Assignee: University of Utah
    Inventors: Robert B. Weiss, Alvin W. Kimball, Raymond F. Gesteland, F. Mark Ferguson, Diane M. Dunn, Leonard J. Di Sera, Joshua L. Cherry