Abstract: Molecular constructs, populations thereof, arrays, compositions, methods and kits for differentiating a target molecule from an off-target molecule are provided.

Abstract: Molecular constructs, populations thereof, arrays, compositions, methods and kits for differentiating a target molecule from an off-target molecule are provided.

Abstract: Molecular constructs, populations thereof, arrays, compositions, methods and kits for differentiating a target molecule from an off-target molecule are provided.

Abstract: Conformationally tunable affinity ligands are rationally designed and selected for the ability to switch under operator-defined environmental conditions between or among structurally distinct states that have different affinities for a given target substance. Tunable affinity ligands are incorporated into reagents, separation media, assays, sensors, devices, kits and systems for sorting, separating, detecting, sensing, quantifying, identifying and monitoring target substances. Applications include biomedical research, diagnostics, drug discovery, bioproduction and processing and environmental, industrial, chemical, agricultural and military use.

Abstract: Separation of antibodies, antibody fragments, and conjugates thereof using multistate affinity ligands rationally designed and selected to undergo analytically and functionally definable conformational transitions from a first affinity state under a first operator-defined environmental condition to a second affinity state under a second operator-defined environmental condition.

Abstract: An immobilized bimolecular construct comprises a solid support, a first oligonucleotide and a second oligonucleotide. The first oligonucleotide is labeled at one end with a fluorophore or quencher and attached at the other end to a solid support. The second oligonucleotide is labeled at one end with a fluorophore or quencher and hybridized at the other end to the first oligonucleotide. Hybridization of the second oligonucleotide with the first oligonucleotide brings the labeled end of the second oligonucleotide in close proximity or physical contact with the labeled end of the first oligonucleotide. In one embodiment the second oligonucleotide is also attached to the solid support in proximity to the first oligonucleotide. In this embodiment, the second oligonucleotide may be first attached to the solid support and then hybridized to the first oligonucleotide or, conversely, first hybridized to the first oligonucleotide and then attached to the solid support.