Patents by Inventor Leszek Alexander Lyznik
Leszek Alexander Lyznik has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7364902Abstract: The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.Type: GrantFiled: April 7, 2006Date of Patent: April 29, 2008Assignee: Pioneer Hi-Bred International, Inc,Inventors: Christopher L. Baszczynski, Leszek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan
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Patent number: 7223601Abstract: Compositions and methods for introducing a DNA of interest into a genomic target site are provided. In particular, the methods and compositions involve the use of a combination of target sites for two site specific recombinases and expression of a chimeric recombinase with dual target site specificity. Thus, the compositions comprise novel site-specific recombinases with specificities to multiple target sites, and nucleotide sequences and expression cassettes encoding these recombinases or target sites. The methods involve transforming a eukaryotic cell having target sites for the novel recombinase with a DNA of interest that is flanked by corresponding target sites. Expression of the recombinase results in integration of the DNA of interest into the genome of the cell. The compositions and methods of the invention have use in the construction of stably transformed eukaryotic cells, and in particular, plant cells.Type: GrantFiled: January 29, 2003Date of Patent: May 29, 2007Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Leszek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan, Aragula Gururaj Rao, Laura A. Tagliani
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Patent number: 7179599Abstract: The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.Type: GrantFiled: May 23, 2003Date of Patent: February 20, 2007Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Leszek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan
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Publication number: 20040261145Abstract: The methods of the invention provide a means for targeting the insertion of a nucleotide sequence of interest to a specific chromosomal site within the genome of a plant cell. The invention provides a unique application of wide hybridization and site-specific recombination to bring together and recombine well defined chromosomal fragments. The invention provides novel methods to generate transgenic plant lines and new hybrid plant varieties.Type: ApplicationFiled: July 21, 2004Publication date: December 23, 2004Applicants: Pioneer Hi-Bred International, Inc., Plant Breeding and Acclimatization InstituteInventors: Leszek Alexander Lyznik, Christopher L. Baszczynski, Waclaw Orczyk
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Publication number: 20040005713Abstract: The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.Type: ApplicationFiled: May 23, 2003Publication date: January 8, 2004Applicant: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Leszek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan
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Patent number: 6664108Abstract: The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.Type: GrantFiled: October 1, 1999Date of Patent: December 16, 2003Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Leszek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan
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Publication number: 20030119166Abstract: Compositions and methods for introducing a DNA of interest into a genomic target site are provided. In particular, the methods and compositions involve the use of a combination of target sites for two site specific recombinases and expression of a chimeric recombinase with dual target site specificity. Thus, the compositions comprise novel site-specific recombinases with specificities to multiple target sites, and nucleotide sequences and expression cassettes encoding these recombinases or target sites. The methods involve transforming a eukaryotic cell having target sites for the novel recombinase with a DNA of interest that is flanked by corresponding target sites. Expression of the recombinase results in integration of the DNA of interest into the genome of the cell. The compositions and methods of the invention have use in the construction of stably transformed eukaryotic cells, and in particular, plant cells.Type: ApplicationFiled: January 29, 2003Publication date: June 26, 2003Applicant: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Leszek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan, Aragula Gururaj Rao, Laura A. Tagliani
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Patent number: 6541231Abstract: Compositions and methods for introducing a DNA of interest into a genomic target site are provided. In particular, the methods and compositions involve the use of a combination of target sites for two site specific recombinases and expression of a chimeric recombinase with dual target site specificity. Thus, the compositions comprise novel site-specific recombinases with specificities to multiple target sites, and nucleotide sequences and expression cassettes encoding these recombinases or target sites. The methods involve transforming a eukaryotic cell having target sites for the novel recombinase with a DNA of interest that is flanked by corresponding target sites. Expression of the recombinase results in integration of the DNA of interest into the genome of the cell. The compositions and methods of the invention have use in the construction of stably transformed eukaryotic cells, and in particular, plant cells.Type: GrantFiled: October 12, 1999Date of Patent: April 1, 2003Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Leszek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan, Aragula Gururaj Rao, Laura A. Tagliani
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Publication number: 20020132350Abstract: Compositions and methods for targeted genetic manipulation of an organism are provided. The compositions are novel integration vectors derived from the Mu bacteriophage comprising an active cleaved donor complex (CDC) and further comprising a targeting mechanism whereby integration of the Mu transposable cassette may be directed to a predetermined target site within a host organism's genome. These integration vectors comprise a Mu transposable cassette and one or more navigator elements that direct targeted insertion of the CDC. Methods of the invention utilize the integration vectors of the invention to insert the Mu transposable cassette into a target site of an organism's genome. This insertion occurs in the absence of the MuB accessory protein. The methods are useful for modulating activity of known genes and for targeting integration of nucleotide sequences of interest into a specific location of an organism's genome. Accordingly, the methods may also be used to create gene disruptions and knockouts.Type: ApplicationFiled: September 13, 2001Publication date: September 19, 2002Applicant: Pioneer Hi-Bred International, Inc.Inventors: Hideki Suzuki, Leszek Alexander Lyznik
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Publication number: 20020023278Abstract: The methods of the invention provide a means for targeting the insertion of a nucleotide sequence of interest to a specific chromosomal site within the genome of a plant cell. The invention provides a unique application of wide hybridization and site-specific recombination to bring together and recombine well defined chromosomal fragments. The invention provides novel methods to generate transgenic plant lines and new hybrid plant varieties.Type: ApplicationFiled: May 7, 2001Publication date: February 21, 2002Inventors: Leszek Alexander Lyznik, Christopher L. Baszczynski, Waclaw Orczyk
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Patent number: 6331661Abstract: The present invention is drawn to compositions and methods for introducing nucleotide sequences at preferred genomic target sites in a eukaryotic genome. The compositions comprise transfer cassettes which are flanked by nonhomologous recombination sites. The method involves transforming eukaryotic cells containing target sites utilizing non-integrating transformation methods. The method results in efficient integration of nucleotides into predetermined genetic locations and eliminates random DNA integration.Type: GrantFiled: November 17, 1998Date of Patent: December 18, 2001Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Leszek Alexander Lyznik, William J. Gordon-Kamm
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Patent number: 6300545Abstract: The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons from T-DNA. The methods comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.Type: GrantFiled: November 17, 1998Date of Patent: October 9, 2001Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Leszek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan
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Patent number: 6262341Abstract: Compositions and methods for introducing a DNA of interest into a genomic target site are provided. In particular, the methods and compositions involve the use of a combination of target sites for two site specific recombinases and expression of a chimeric recombinase with dual target site specificity. Thus, the compositions comprise novel site-specific recombinases with specificities to multiple target sites, and nucleotide sequences and expression cassettes encoding these recombinases or target sites. The methods involve transforming a eukaryotic cell having target sites for the novel recombinase with a DNA of interest that is flanked by corresponding target sites. Expression of the recombinase results in integration of the DNA of interest into the genome of the cell. The compositions and methods of the invention have use in the construction of stably transformed eukaryotic cells, and in particular, plant cells.Type: GrantFiled: November 17, 1998Date of Patent: July 17, 2001Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Leszek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan, Argula Gururaj Rao, Laura A. Tagliani