Abstract: This patent application belongs to the field of tissue engineering. In particular, it refers to a method of producing decellularized biomaterial from biological tissue, as well as to the decellularized biomaterial produced by said method comprising biological extracellular matrix. Particularly, said biological tissue derives from animal or human ocular connective tissue. More particularly, it refers to a method of producing decellularized biomaterial from animal sclera for producing a biological scaffold, prosthesis and/or carrier of active substances, as well as biomaterial itself and uses thereof as scaffolds, prosthesis or carriers of substances for systemic or localized release.