Patents by Inventor Lev Kotler
Lev Kotler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20190323078Abstract: The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads. The invention further provides sets of labeled extension probes containing phosphorothiolate linkages.Type: ApplicationFiled: May 7, 2019Publication date: October 24, 2019Inventors: Kevin McKernan, Alan BLANCHARD, Lev KOTLER, Gina COSTA
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Patent number: 10323277Abstract: The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads.Type: GrantFiled: October 12, 2016Date of Patent: June 18, 2019Assignee: APPLIED BIOSYSTEMS, LLCInventors: Kevin McKernan, Alan Blanchard, Lev Kotler, Gina Costa
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Publication number: 20170081717Abstract: The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads.Type: ApplicationFiled: October 12, 2016Publication date: March 23, 2017Inventors: Kevin McKernan, Alan BLANCHARD, Lev KOTLER, Gina COSTA
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Patent number: 9493830Abstract: The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads. The invention further provides sets of labeled extension probes containing phosphorothiolate linkages.Type: GrantFiled: October 18, 2013Date of Patent: November 15, 2016Assignee: Applied Biosystems, LLCInventors: Kevin McKernan, Alan Blanchard, Lev Kotler, Gina Costa
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Patent number: 9217177Abstract: The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to beads. The invention further provides sets of labeled extension probes containing phosphorothiolate linkages.Type: GrantFiled: January 9, 2013Date of Patent: December 22, 2015Assignee: APPLIED BIOSYSTEMS, LLCInventors: Kevin McKernan, Alan Blanchard, Lev Kotler, Gina Costa
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Publication number: 20140248610Abstract: The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads. The invention further provides sets of labeled extension probes containing phosphorothiolate linkages.Type: ApplicationFiled: October 18, 2013Publication date: September 4, 2014Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Kevin McKernan, Alan Blanchard, Lev Kotler, Gina Costa
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Patent number: 8431691Abstract: The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads. The invention further provides sets of labeled probes containing phosphorothiolate linkages.Type: GrantFiled: July 21, 2008Date of Patent: April 30, 2013Assignee: Applied Biosystems LLCInventors: Kevin McKernan, Alan Blanchard, Lev Kotler, Gina Costa
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Patent number: 8329404Abstract: The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads. The invention further provides sets of labeled extension probes containing phosphorothiolate linkages.Type: GrantFiled: March 2, 2012Date of Patent: December 11, 2012Assignee: Applied Biosystems LLCInventors: Kevin McKernan, Alan Blanchard, Lev Kotler, Gina Costa
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Publication number: 20120191363Abstract: The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads. The invention further provides sets of labeled extension probes containing phosphorothiolate linkages.Type: ApplicationFiled: March 2, 2012Publication date: July 26, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: KEVIN MCKERNAN, ALAN BLANCHARD, LEV KOTLER, GINA COSTA
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Publication number: 20110096620Abstract: A vortex mixer and method for forming an emulsion wherein the mixer is adapted to form an emulsion with a desired droplet size and having a desired volume. The vortex mixer provides improved uniformity in emulsion preparation and may be used to create multiple emulsions simultaneously.Type: ApplicationFiled: December 29, 2010Publication date: April 28, 2011Applicant: APPLIED BIOSYSTEMS, LLCInventors: Lev KOTLER, John Andrew Sheridan, Gina Costa, Joseph Podhasky
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Publication number: 20110077169Abstract: The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. In certain embodiments the methods make use of extension probes containing an abasic residue or a damaged base and employ agents appropriate to cleave linkages between a nucleoside and an abasic residue and/or agents appropriate to remove a damaged base from a nucleic acid. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle.Type: ApplicationFiled: December 2, 2009Publication date: March 31, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: KEVIN MCKERNAN, ALAN BLANCHARD, LEV Kotler, GINA COSTA
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Patent number: 7883265Abstract: A vortex mixer and method for forming an emulsion wherein the mixer is adapted to form an emulsion with a desired droplet size and having a desired volume. The vortex mixer provides improved uniformity in emulsion preparation and may be used to create multiple emulsions simultaneously.Type: GrantFiled: May 30, 2008Date of Patent: February 8, 2011Assignee: Applied Biosystems, LLCInventors: Lev Kotler, John Andrew Sheridan, Gina Costa, Joseph Podhasky
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Publication number: 20100297626Abstract: The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. In certain embodiments the methods make use of extension probes containing an abasic residue or a damaged base and employ agents appropriate to cleave linkages between a nucleoside and an abasic residue and/or agents appropriate to remove a damaged base from a nucleic acid. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle.Type: ApplicationFiled: November 30, 2009Publication date: November 25, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Kevin MCKERNAN, Alan Blanchard, Lev Kotler, Gina Costa
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Patent number: 7578915Abstract: A modular multiple lane or capillary electrophoresis (chromatography) system that permits automated parallel separation and comprehensive collection of all fractions from samples in all lanes or columns, with the option of further on-line automated sample fraction analysis, is disclosed. Preferably, fractions are collected in a multi-well fraction collection unit, or plate. The multi-well collection plate is preferably made of a solvent permeable gel, most preferably a hydrophilic, polymeric gel such as agarose or cross-linked polyacrylamide.Type: GrantFiled: August 29, 2003Date of Patent: August 25, 2009Assignee: Northeastern UniversityInventors: Barry L. Karger, Lev Kotler, Frantisek Foret, Marek Minarik, Karel Kleparnik
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Publication number: 20090181860Abstract: The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads. The invention further provides sets of labeled probes containing phosphorothiolate linkages.Type: ApplicationFiled: July 21, 2008Publication date: July 16, 2009Applicant: Applied Biosystems Inc.Inventors: Kevin McKernan, Alan Blanchard, Lev Kotler, Gina Costa
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Publication number: 20090181385Abstract: The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads. The invention further provides sets of labeled probes containing phosphorothiolate linkages.Type: ApplicationFiled: July 21, 2008Publication date: July 16, 2009Applicant: Applied Biosystems Inc.Inventors: Kevin McKernan, Alan Blanchard, Lev Kotler, Gina Costa
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Publication number: 20090035825Abstract: A vortex mixer and method for forming an emulsion wherein the mixer is adapted to form an emulsion with a desired droplet size and having a desired volume. The vortex mixer provides improved uniformity in emulsion preparation and may be used to create multiple emulsions simultaneously.Type: ApplicationFiled: May 30, 2008Publication date: February 5, 2009Applicant: Applera CorporationInventors: Lev Kotler, John Andrew Sheridan, Gina Costa, Joseph Podhasky
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Patent number: 7323092Abstract: While the present invention has been described in conjunction with a preferred embodiment, one of ordinary skill, after reading the foregoing specification, will be able to effect various changes, substitutions of equivalents, and other alterations to the compositions and methods set forth herein. It is therefore intended that the protection granted by Letters Patent hereon be limited only by the definitions contained in the appended claims and equivalents thereof.Type: GrantFiled: September 2, 2003Date of Patent: January 29, 2008Assignee: Northeastern UniversityInventors: Barry L. Karger, Lev Kotler, Frantisek Foret, Marek Minarik, Karel Kleparnik
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Publication number: 20080003571Abstract: The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. In certain embodiments the methods make use of extension probes containing an abasic residue or a damaged base and employ agents appropriate to cleave linkages between a nucleoside and an abasic residue and/or agents appropriate to remove a damaged base from a nucleic acid. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle.Type: ApplicationFiled: February 1, 2006Publication date: January 3, 2008Inventors: Kevin McKernan, Alan Blanchard, Lev Kotler, Gina Costa
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Publication number: 20040222095Abstract: The invention is directed to a high throughput nucleic acid separation method using an improved uncrosslinked polymer separation matrix for increasing read length and separation speed, while maintaining accuracy, for, e.g., nucleic acid sequencing. The separation matrix of the invention includes a denaturant comprising dimethyl sulfoxide (DMSO). Preferably, the separation matrix may further comprise urea. Preferred matrix polymers include linear polyacrylamide, poly(ethylene oxide), hydroxyethyl cellulose, poly(dimethylacrylamide) and poly(vinylpyrrolidone).Type: ApplicationFiled: July 9, 2003Publication date: November 11, 2004Inventors: Barry L. Karger, Lev Kotler, Hui He