Abstract: Disclosed herein is an improved automatic sampling and biochemical monitoring system for a microbial or cell culture or a biochemical reaction in a bioreactor.

Abstract: Described herein is an improved automatic bioreactor system utilizing a multi-tray or multi-plate cell culture vessel for cultivating adherent cells.

Abstract: Disclosed herein is an improved automatic bioreactor system utilizing a multi-tray or multi-plate cell culture vessel for cultivating adherent cells.

Abstract: Disclosed herein is an improved automatic sampling and biochemical monitoring system for a microbial or cell culture or a biochemical reaction in a bioreactor.

Abstract: A large scale cell harvesting method for a pack-bed culture device by providing cell growing space that is packed with a plurality of porous matrices and/or matrix means. When a cell detaching step at least comprising shaking or spinning the culture chamber is performed, the unoccupied space is large enough for the porous matrices collide to each other or to the culture chamber to generate momentum whereby the cells detach from the porous matrices to harvest the cells.

Abstract: A large scale cell harvesting method for a pack-bed culture device by providing cell growing space that is loosely packed with a plurality of porous matrixes and/or matrix means. When a cell detaching step at least comprising shaking or spinning the culture chamber is performed, the unoccupied space is large enough for the porous matrixes collide to each other or to the culture chamber to generate momentum whereby the cells detach from the porous matrixes to harvest the cells.

Abstract: A single-used matrix sampling device for the single-used, disposable or autoclavble packed-bed bioreactor is provided herein. The present invention includes a tube means and a matrix collecting means. The matrix collecting means configured for collecting the cell culture matrix includes a receptacle and a pulling means connected to the receptacle or the matrix collecting means could be scoop-like in configuration; the tube means is configured for preventing the matrix collecting means from being contaminated while mechanically or pneumatically sampling; and the tube means could be sealed to enclose the sampled matrix collecting means after sampling. A single-used matrix sampling method for a single-used, disposable or autoclavble packed-bed bioreactor is also provided. The present invention could be utilized to aseptically collect the cell culture matrix from the packed-bed bioreactor without requiring to face the risks of contamination.

Abstract: An external mixing device comprises: arranging an inlet and an outlet on the single-use container; a sterilizable or a single-use pump head connected to the inlet and outlet with a mixer installed inside. Driven by a driving device, the mixer makes the liquid circulate continuously between the pump head and the single-use container. The external mixing device further comprises a sensor chamber installed with a plurality of sensors and extra ports, which are used for introducing solution or gas inside or for sampling and detecting liquid properties. A single-use connector is used to connect the single-use container to the external mixing device, which can be operated aseptically under general environment. The external mixing device is small-sized with the advantage of simplified operation procedures.

Abstract: A scalable packed-bed cell culture device includes a matrix vessel, a mixing vessel, a communicating means, a driving means and a controlling means. The matrix vessel includes porous matrixes packed therein. The mixing vessel includes a mixing means configured for mixing a culture medium. The communicating means is connected between the matrix vessel and the mixing vessel. The driving means is configured for driving the culture medium to flow between the matrix vessel and the mixing vessel. The controlling means configured for controlling the culture medium to submerge the porous matrixes at high level, and to emerge the porous matrixes at low level. An inoculation method and a culture method for scalable packed-bed cell culture device is also herein provided for eliminating the limitation of aeration or oxygenation during culture, alleviating the gradient effect, eliminating the channeling effect in conventional packed-bed bioreactors.

Abstract: The present invention discloses a three-dimensional porous growth surface made from polysaccharide material, especially the alginic acid, to enhance cell growth surface, promote cell adherence, immobilization and propagation, maintain surface structure integrity, enable programmable degradation, and thus increase cellular production. The present invention teaches several methods: a method to enhance the integrity of the growth surface by protecting the growth surface in a rigid solid support; a method of use for enhancing the performance of the surface; and a method of modifying a growth surface for eukaryotic and/or prokaryotic cells comprising the steps of increasing surface area by creating porous and 3-D structure, treating a surface to encourage cell attachment, promoting cell growth and proliferation, and disposing the growth surface in any conventional cell cultivating device. The growth surface is able to program degradation and release the cell/tissue mass after the culture is completed.

Abstract: An apparatus and method for preparing and culturing cells includes a chamber having an inlet and an outlet for introducing culture medium and air. A cell growth substrate placed in the chamber for cells anchored or embedded. The preferred embodiment of invention claims a novel process for controlling the platform form to tilt at one end, to maintain the seesaw and rocking movement for cultured process. The seesaw movement or rocking movement efficiently the nutrition, carbon dioxide and oxygen transfer during cultured process for production of cellular protein products or harvesting the cells.

Abstract: The present invention relates to a method for the preparation of a Neutrophil Inhibitory Factor (NIF) comprising the cultivation of mammalian cells expressing NIF in an animal component-free growth medium. The present invention may be employed in large-scale preparation of NIF. The invention also relates to a method for the preparation of recombinant proteins comprising the cultivation of mammalian cells expressing an exogenous recombinant protein in an animal component-free growth medium.

Abstract: The present invention relates to a method for the preparation of a Neutrophil Inhibitory Factor (NIF) comprising the cultivation of mammalian cells expressing NIF in an animal component-free growth medium. The present invention may be employed in large-scale preparation of NIF. The invention also relates to a method for the preparation of recombinant proteins comprising the cultivation of mammalian cells expressing an exogenous recombinant protein in an animal component-free growth medium.

Abstract: An extensive morse code processing system includes a microprocessor unit, a transmitter processing unit, a receiver processing unit, a display unit, a keyboard unit, and a radio unit. Whenever a data key on the keyboard is pressed, the ASCII code of the pressed key is received by the microprocessor unit and is converted into a binary line code. The line code is processed by the transmitter unit and is then transmitted as morse code signals by the radio unit. Morse code signals received by the radio unit are converted by the receiver unit into the corresponding binary line code. The line code from the receiver unit serves as input to the microprocessor unit which converts the line code into standard ASCII. The microprocessor then actuates the display unit to display the received characters. Exchange of data between the microprocessor unit and a a computer is also possible through an RS-232 interface adapter interconnecting the computer and the microprocessor unit.