Abstract: Provided is a method, including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments. Also provided is a method including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments.

Abstract: Provided is a method, including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments. Also provided is a method including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments.

Abstract: In one example, a method of preparing a fluidic channel includes covalently coupling a first region of a substrate to a first region of a cover using first moieties covalently coupled to the first region of the substrate and second moieties covalently coupled to the first region of the cover. The covalent coupling between the first region of the substrate and the first region of the cover suspends a second region of the cover over a second region of the substrate to form a fluidic channel.

Abstract: An image sensor structure includes an image layer having an array of light detectors disposed therein. A device stack is disposed over the image layer. An array of light guides is disposed in the device stack. Each light guide is associated with a light detector. An array of nanowells is disposed over the device stack. Each nanowell is associated with a first light guide of the array of light guides. A first primer set is disposed throughout a first well region of each nanowell. A second primer set is disposed throughout a second well region of each nanowell. The second well region is adjacent the first well region. The first and second primer sets are operable to attach a forward strand cluster of forward polynucleotide strands in the first well region and a reverse strand cluster of reverse polynucleotide strands in the second well region.

Abstract: Provided is a method, including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments. Also provided is a method including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments.

Abstract: This disclosure generally relates to repeaters, and, in particular, repeaters for open-drain systems. In one embodiment, an apparatus comprises a first port, a second port, a current detector, a transistor, and a control logic circuit. A current detector input of the current detector is coupled to the first port. A transistor channel electrode of the transistor is coupled to the second port. A control logic circuit input of the control logic circuit is coupled to the current detector output, and a control logic circuit output of the control logic circuit is coupled to a transistor control electrode of the transistor.

Abstract: This disclosure generally relates to repeaters, and, in particular, repeaters for open-drain systems. In one embodiment, an apparatus comprises a first port, a second port, a current detector, a transistor, and a control logic circuit. A current detector input of the current detector is coupled to the first port. A transistor channel electrode of the transistor is coupled to the second port. A control logic circuit input of the control logic circuit is coupled to the current detector output, and a control logic circuit output of the control logic circuit is coupled to a transistor control electrode of the transistor.

Abstract: This disclosure generally relates to repeaters, and, in particular, repeaters for open-drain systems. In one embodiment, an apparatus comprises a first port, a second port, a current detector, a transistor, and a control logic circuit. A current detector input of the current detector is coupled to the first port. A transistor channel electrode of the transistor is coupled to the second port. A control logic circuit input of the control logic circuit is coupled to the current detector output, and a control logic circuit output of the control logic circuit is coupled to a transistor control electrode of the transistor.

Abstract: This disclosure generally relates to repeaters, and, in particular, repeaters for open-drain systems. In one embodiment, an apparatus comprises a first port, a second port, a current detector, a transistor, and a control logic circuit. A current detector input of the current detector is coupled to the first port. A transistor channel electrode of the transistor is coupled to the second port. A control logic circuit input of the control logic circuit is coupled to the current detector output, and a control logic circuit output of the control logic circuit is coupled to a transistor control electrode of the transistor.

Abstract: This disclosure generally relates to repeaters, and, in particular, repeaters for open-drain systems. In one embodiment, an apparatus comprises a first port, a second port, a current detector, a transistor, and a control logic circuit. A current detector input of the current detector is coupled to the first port. A transistor channel electrode of the transistor is coupled to the second port. A control logic circuit input of the control logic circuit is coupled to the current detector output, and a control logic circuit output of the control logic circuit is coupled to a transistor control electrode of the transistor.

Abstract: This disclosure generally relates to repeaters, and, in particular, repeaters for open-drain systems. In one embodiment, an apparatus comprises a first port, a second port, a current detector, a transistor, and a control logic circuit. A current detector input of the current detector is coupled to the first port. A transistor channel electrode of the transistor is coupled to the second port. A control logic circuit input of the control logic circuit is coupled to the current detector output, and a control logic circuit output of the control logic circuit is coupled to a transistor control electrode of the transistor.

Abstract: T cells are profiled with respect to their expression of antigen receptor. The cells are arrayed on a planar or three-dimensional substrate through binding to immobilized or partially diffused MHC-antigen complexes. The cells may further be characterized with respect to their ability to respond to external stimulus in the microenvironment. External stimuli include cell-cell interactions, response to factors, and the like.