Abstract: An improved device for replicating cultures of microorganisms grown in petri plates which pierces colonies with an array of fine needles. The device is capable of at least ten replications, and will replicate virtually any colony.

Abstract: An easily loaded culture plate for microorganisms with an unconstrained flat agar surface for sampling flat areas.

Abstract: A method and composition is provided to control fungal infection of seeds and seedlings. Certain species of Streptomyces are grown on dried ground shrimp waste preferably suspended in a mineral salts medium. The supernate derived from the culture provides fungal infection control when used as a seed-coating or seed-soaking material. The residue, consisting of unused shrimp waste and a Streptomyces species, also provides protection against fungal infection of plants such as cotton seedlings. The residue is employed as a amendment to the soil in the immediate area of the newly planted seed.

Abstract: A method of treating a subterranean oil-bearing formation having an in-situ microbial population therein. In a preferred embodiment, the method permits increased oil recovery by stimulating the growth of the in-situ microbial populations. Nutrients, including nitrogenous-and phosphorous-containing compounds, are injected into the formation concurrent with or subsequent to a waterflood. The nutrients can be injected sequentially, and the sequence can be varied or repeated as necessary. Growth of the microorganisms increases displacement and sweep efficiency of a subsequent waterflood.

Abstract: A perforated gel-supporting structure with a temporary seal attached to it divides a cylindrical dish into cylindrical compartments A and B. Microorganisms are grown on agar poured into compartment A. The dish is inverted, the seal removed, and a second nutrient medium is poured into compartment B. Antibiotic substances produced by the microorganisms diffuse through the agar and inhibit growth of pathogens which have been inoculated into compartment B.