Abstract: Certain disclosed embodiments concern a bioremediation composition comprising microbial cells, at least one co-metabolism substrate to induce selected enzyme production by the microbial cells, and a bead or gel encapsulating the microbial cells, such as bacterial or fungi cells, and the at least one co-metabolism substrate. For certain embodiments, the substrate is a slow release compound, such as an orthosilicate that hydrolyzes to produce an alcohol growth substrate. Embodiments of a method for using the composition to transform contaminants of concern also are disclosed.

Abstract: Certain disclosed embodiments concern a bioremediation composition comprising microbial cells, at least one co-metabolism substrate to induce selected enzyme production by the microbial cells, and a bead or gel encapsulating the microbial cells, such as bacterial or fungi cells, and the at least one co-metabolism substrate. For certain embodiments, the substrate is a slow release compound, such as an orthosilicate that hydrolyzes to produce an alcohol growth substrate. Embodiments of a method for using the composition to transform contaminants of concern also are disclosed.

Abstract: Certain disclosed embodiments concern a bioremediation composition comprising microbial cells, at least one co-metabolism substrate to induce selected enzyme production by the microbial cells, and a bead or gel encapsulating the microbial cells, such as bacterial or fungi cells, and the at least one co-metabolism substrate. For certain embodiments, the substrate is a slow release compound, such as an orthosilicate that hydrolyzes to produce an alcohol growth substrate. Embodiments of a method for using the composition to transform contaminants of concern also are disclosed.

Abstract: Bioremediation methods and kits for degrading environmental contaminants, such as chlorinated hydrocarbons, in a sample containing microorganisms uses at least one slow release compound having at least one hydrolyzable organic group capable of forming at least one alcohol, at least one organic acid, or a combination thereof, upon hydrolysis. The hydrolysis occurs over an extended period of time to provide alcohols and/or organic acids, which are used by the microorganisms to degrade the environmental contaminants.

Abstract: A system for in-situ bioremediation includes a well extending into a saturated zone. The well includes a casing having two permeable sections in the saturated zone. Groundwater may be recirculated through the well and the saturated zone between the two permeable sections. Nutrients, such as oxygen and methane, may be added to the groundwater as it flows in the well. A venturi transfer device may be used to supply the nutrients.

Abstract: The present invention is directed to a method for purifying an underground water supply containing halogenated aliphatic compounds by in-situ biodegradation of said compounds, comprising injecting water into an aquifer zone, the injected water containing an electron donor capable of being utilized by an indigenous population of microorganisms contained in the groundwater supply as a primary growth substrate, and an electron acceptor for oxidation of the primary substrate, by separate addition of electron donor and electron acceptor temporally or spatially to stimulate growth of the microorganisms and to distribute them throughout the aquifer zone to cause in-situ degradation of the halogenated aliphatic compounds by metabolic activity of the microorganisms.