Abstract: This disclosure relates to a biosensor for detecting a target analyte in a sample comprising: a double-stranded oligonucleotide comprising an overhang on a first strand of the oligonucleotide, and a second strand of the oligonucleotide that is a reporter moiety comprising a detectable label; a first detection probe comprising a recognition moiety and a junction forming moiety, wherein the junction forming moiety comprises a first portion capable of binding by complementarity to the overhang of the first strand of the double-stranded oligonucleotide; a second detection probe comprising a recognition moiety and a junction forming moiety, wherein the junction forming moiety comprises a first portion capable of binding by complementarity to an internal segment of the first strand of the double-stranded oligonucleotide; and a capture probe functionalized on an electrode, wherein the capture probe comprises an immobilized strand attached to the electrode. Methods and uses thereof are also disclosed herein.

Abstract: Provided herein is a biosensor for detecting a target analyte in a sample comprising a first and second photoelectrode each comprising conductive substrate and photoactive material, a first and second capture probe functionalized on the first and second photoelectrode, respectively, and optionally one or more reporter moieties comprising a detectable label, wherein the first and second capture probe each, independently, provides a distance between the detectable label and the photoactive material in the presence of the target analyte, wherein intensity of detection signal dictated by the distance is generated from the first and second photoelectrode by transfer of electrons between the detectable label and the photoactive material, wherein a higher, or higher increase than in absence of sample, in the intensity of the detection signal from the first as compared to the second photoelectrode in the presence of the sample, is indicative of the presence of the target analyte.

Abstract: This application relates to a photoactive product comprising: a catechol-containing compound, an amine-containing polymer, and photoactive material. The photoactive product can act as a photocatalyst and can be used in photoelectrodes for use in, for example, photoelectrochemical analyte sensing, including biosensing. The photoelectrodes modified with hybrid organic/inorganic materials can provide increased light absorption and charge separation, binding sites for attaching biorecognition probes, and built-in film-forming properties for well-adhered and uniform photoactive frameworks on the collector electrodes.

Abstract: Methods for diagnosing or monitoring endometriosis in a mammal are provided. The methods include the steps of determining the expression levels of BDNF, glycodelin and optionally ZAG, in a biological sample from the mammal, and determining that the mammal has endometriosis when the biomarker expression levels in the sample are elevated.

Abstract: This application relates to omniphobic materials which are physically and chemically modified at their surface to create hierarchically structured materials with both nanoscale and microscale structures that provide the omniphobic properties. Methods of making such omniphobic surfaces with hierarchical structures and uses thereof, including as flexible films that repel contaminants are also disclosed in the application.

Abstract: The present application relates to a biosensor for detecting an analyte comprising a first and second working electrode; a detection probe functionalized on the first working electrode, the detection probe comprising a reporter moiety and a recognition moiety for an analyte; a capture probe functionalized on the second working electrode; and a counter electrode. Each working electrode is configured to provide a change in signal if the analyte is present. The biosensor can be used to detect an analyte in a sample.

Abstract: This disclosure relates to a biosensor, and methods of use thereof, for detecting a target in a sample comprising a photoelectrode comprising a conductive substrate and a photoactive material; a population of capture probes functionalized on the photoelectrode wherein the capture probes are capable of binding to the target and a reporter moiety; and the reporter moiety comprising a detectable label and a capture probe binding portion; wherein exposure of the target to the population of the capture probes results in binding of the target to a fraction of the population which results in a decrease in detection signal intensity compared to the intensity in the absence of the target, and subsequent binding of the reporter moiety to the remaining unbound capture probes results in an increase in detection signal intensity that is less than an increase from the reporter moiety binding to capture probes not exposed to the target.

Abstract: This disclosure relates to a biosensor for detecting a target analyte in a sample comprising a first and second photoelectrode each comprising conductive substrate and photoactive material, a first and second capture probe functionalized on the first and second photoelectrode, respectively, and optionally one or more reporter moieties comprising a detectable label, wherein the first and second capture probe each, independently, provides a distance between the detectable label and the photoactive material in the presence of the target analyte, wherein intensity of detection signal dictated by the distance is generated from the first and second photoelectrode by transfer of electrons between the detectable label and the photoactive material, wherein a higher, or higher increase than in absence of sample, in the intensity of the detection signal from the first as compared to the second photoelectrode in the presence of the sample, is indicative of the presence of the target analyte.

Abstract: Methods for diagnosing or monitoring endometriosis in a mammal are provided. The methods include the steps of determining the expression levels of BDNF, glycodelin and optionally ZAG, in a biological sample from the mammal, and determining that the mammal has endometriosis when the biomarker expression levels in the sample are elevated.

Abstract: An all-solution electrode fabrication process is provided. The process comprises the steps of: i) preparing and activating a shrinkable polymer substrate for deposition of a a) conductive film; ii) modifying the substrate to incorporate a linker; iii) immobilizing particles of a conductive material on the linkers of the substrate to form a conductive film on the substrate; and vi) heating the modified substrate to a temperature sufficient to cause contraction of the polymer substrate and to result in micro- and/or nano-texturing in the conductive film. The process advantageously yields a novel multi-scale electrode device comprising a polymer substrate; and a textured electro-conductive film linked to the substrate.

Abstract: A device for manipulating magnetic particles, and the method of fabricating and use thereof. The device includes a substrate; a conductive element formed onto the substrate in a pattern shaped to enhance a magnetic field generated in response to an applied current; an insulating layer to isolate the conductive element from a magnetic element; and a magnetic element formed onto the insulating layer to enhance a magnetic force resulting from the magnetic field generated by the conductive element. The magnetic element can be shaped similarly to the conductive element, and edges of the magnetic element are substantially aligned with edges of the conductive element. During fabrication, the substrate and the conductive element can be heated to cause the substrate to shrink thereby resulting in a wrinkled structure at the conductive element. The device can be used to manipulate the magnetic particles within a biological sample, such as cells and/or biomolecules.

Abstract: An all-solution electrode fabrication process is provided, The process comprises the steps of: i) preparing and activating a shrinkable polymer substrate for deposition of a a) conductive film; ii) modifying the substrate to incorporate a linker; iii) immobilizing particles of a conductive material on the linkers of the substrate to form a conductive film on the substrate; and vi) heating the modified substrate to a temperature sufficient to cause contraction of the polymer substrate and to result in micro- and/or nano-texturing in the conductive film. The process advantageously yields a novel multi-scale electrode device comprising a polymer substrate; and a textured electro-conductive film linked to the substrate.

Abstract: Nanostructured microelectrodes and biosensing devices incorporating the same are disclosed herein.

Abstract: Methods for diagnosing or monitoring endometriosis in a mammal are provided. The methods include the steps of determining the expression levels of BDNF, glycodelin and optionally ZAG, in a biological sample from the mammal, and determining that the mammal has endometriosis when the biomarker expression levels in the sample are elevated.

Abstract: Nanostructured microelectrodes and biosensing devices incorporating the same are disclosed herein.

Abstract: Nanostructured microelectrodes and biosensing devices incorporating the same are disclosed herein.

Abstract: A device for manipulating magnetic particles, and the method of fabricating and use thereof. The device includes a substrate; a conductive element formed onto the substrate in a pattern shaped to enhance a magnetic field generated in response to an applied current; an insulating layer to isolate the conductive element from a magnetic element; and a magnetic element formed onto the insulating layer to enhance a magnetic force resulting from the magnetic field generated by the conductive element. The magnetic element can be shaped similarly to the conductive element, and edges of the magnetic element are substantially aligned with edges of the conductive element. During fabrication, the substrate and the conductive element can be heated to cause the substrate to shrink thereby resulting in a wrinkled structure at the conductive element. The device can be used to manipulate the magnetic particles within a biological sample, such as cells and/or biomolecules.

Abstract: Nanostructured microelectrodes and biosensing devices incorporating the same are disclosed herein.

Abstract: Nanostructured microelectrodes and biosensing devices incorporating the same are disclosed herein.

Abstract: Nanostructured microelectrodes and biosensing devices incorporating the same are disclosed herein.