Abstract: Provided herein are devices, methods, and systems for in situ gene sequencing of a target nucleic acid in a cell in an intact tissue. Methods of screening a candidate agent to determine whether the candidate agent modulates gene expression of a nucleic acid in a cell in an intact tissue are also provided herein.

Abstract: Provided are a determination method and apparatus of a main shaft bearing state, an electronic device, and a storage medium. The method includes the following steps: Bearing swing data of a target main shaft bearing at multiple moments within a preset time period are acquired; at least one current data period and current-period bearing swing data in each current data period are determined according to the multiple pieces of bearing swing data; a first abrupt change value and a second abrupt change value are determined according to the current-period bearing swing data in each current data period; and a main shaft bearing state of the target main shaft bearing is determined according to the first abrupt change value and the second abrupt change value.

Abstract: The invention provides novel methods for analyzing (e.g., imaging) drugs in mammalian tissues, and methods for identifying drug targets and analyzing drug-target interactions.

Abstract: One example magnetic random access memory includes a plurality of structural units and a plurality of voltage control lines. The plurality of voltage control lines are in parallel with each other. Planes in which the plurality of structural units are located are in parallel with each other, and a plane in which each of the plurality of structural units is located is perpendicular to the plurality of voltage control lines. Each structural unit includes a multi-layer storage structure including multiple layers that are stacked in sequence. Each layer of the multi-layer storage structure includes an electrode line and a plurality of storage units disposed on the electrode line. Each of the plurality of storage units includes a magnetic tunnel junction. A first end of each storage unit is connected to the electrode line, and a second end of each storage unit is connected to one of the plurality of voltage control lines.

Abstract: Provided are a determination method and apparatus of a main shaft bearing state, an electronic device, and a storage medium. The method includes the following steps: Bearing swing data of a target main shaft bearing at multiple moments within a preset time period are acquired; at least one current data period and current-period bearing swing data in each current data period are determined according to the multiple pieces of bearing swing data; a first abrupt change value and a second abrupt change value are determined according to the current-period bearing swing data in each current data period; and a main shaft bearing state of the target main shaft bearing is determined according to the first abrupt change value and the second abrupt change value.

Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.

Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.

Abstract: Provided are a high-entropy carbide ceramic, a rare earth-containing high-entropy carbide ceramic, fibers thereof, precursors thereof, and preparation methods thereof. The precursor includes at least four elements selected from Ti, Zr, Hf, V, Nb, Ta, Mo, and W, with each metal element accounting for 5-35% of the total molar quantity of metal elements in the precursor. The rare earth-containing high-entropy carbide ceramic precursor includes at least four transition metal elements and at least one rare-earth metal element. The high-entropy ceramic is a single-crystal-phase high-performance ceramic prepared from the precursor, with each element being homogenously distributed at molecular level.

Abstract: Example magnetic random access memories are described. One example magnetic random access memory includes a plurality of structural units and a plurality of voltage control lines. The plurality of voltage control lines are in parallel with each other. Planes in which the plurality of structural units are located are in parallel with each other, and a plane in which each of the plurality of structural units is located is perpendicular to the plurality of voltage control lines. Each structural unit includes a multi-layer storage structure including multiple layers that are stacked in sequence. Each layer of the multi-layer storage structure includes an electrode line and a plurality of storage units disposed on the electrode line. Each of the plurality of storage units includes a magnetic tunnel junction. A first end of each storage unit is connected to the electrode line, and a second end of each storage unit is connected to one of the plurality of voltage control lines.

Abstract: Disclosed are a high-entropy nitride ceramic fiber, and a preparation method and use thereof. The high-entropy ceramic fiber comprises Ti, Hf, Ta, Nb, and Mo; the high-entropy nitride ceramic fiber presents single crystal phase, and each of the elements are uniformly distributed at molecular level. The preparation method of the high-entropy ceramic fiber comprises: mixing a high-entropy ceramic precursor comprising the target metal elements, a spinning aid, and a solvent uniformly to prepare a precursor spinning solution, followed by working procedures of spinning, pyrolyzation, and nitriding to prepare the high-entropy nitride ceramic fiber. The high-entropy nitride ceramic fiber can be used in photocatalysis process of carbon dioxide to prepare methane.

Abstract: A composition for preparing polyisocyanurate and polyurethane foams is provided, comprising A) an isocyanate-reactive component, B) a polyisocyanate component and C) a branched siloxane comprising at least three trimethylsiloxy groups. A method for preparing the polyisocyanurate and polyurethane foams, and foams prepared thereby are also provided.

Abstract: Method and device of storage capacity evaluation based on a CDN application are provided. The method includes: for any cache server corresponding to a target service, using files stored before a preset time node as old files in the cache server; determining a proportion of the old files in all files stored by the cache server and a visiting rate of the old files within a preset visit time range; determining an adjustment type of a storage capacity of the cache server according to the proportion of the old files and the visiting rate of the old files, where the adjustment type at least includes capacity to-be-expanded, capacity to-be-reduced and capacity unchanged; and determining an adjustment type of a storage capacity of the target service according to adjustment types of storage capacities of all cache servers corresponding to the target service.

Abstract: The present disclosure provides a method and a device of storage capacity evaluation based on a CDN application. The method includes: for any cache server corresponding to a target service, using files stored before a preset time node as old files in the cache server; determining a proportion of the old files in all files stored by the cache server and a visiting rate of the old files within a preset visit time range; determining an adjustment type of a storage capacity of the cache server according to the proportion of the old files and the visiting rate of the old files, where the adjustment type at least includes capacity to-be-expanded, capacity to-be-reduced and capacity unchanged; and determining an adjustment type of a storage capacity of the target service according to adjustment types of storage capacities of all cache servers corresponding to the target service. The disclosure simultaneously considers storage cost and bandwidth cost and may also evaluate different services.

Abstract: The present disclosure provides nucleic acid activity-dependent expression vectors and activity-dependent expression cassettes for the activity-dependent expression of an encoded polypeptide. Also provided are recombinant adenoassociated viruses (AAV) containing an expression vector comprising an activity-dependent expression cassette for the activity-dependent expression of an encoded polypeptide by cells infected with the AAV vector. The present disclosure also provides methods for the activity-dependent labeling of cells in vitro or in vivo by introducing into the cells an expression vector containing an activity-dependent regulatory sequence driving expression of a labeling polypeptide. Also provided are methods for the activity-dependent control of cells in vitro or in vivo by introducing into the cells an expression vector containing an activity-dependent regulatory sequence driving expression of a light-responsive polypeptide.

Abstract: A dual-nuclear radio frequency (RF) coil device includes a first RF coil and a second RF coil. The first RF coil includes at least one adjustment capacitor, the first RF coil is configured to generate a first magnetic field, and a direction of a primary magnetic field of the first magnetic field is a first direction. The second RF coil includes an electric dipole and a tuning and matching circuit connected between two conductors of the electric dipole. The second RF coil is configured to generate a second magnetic field and a direction of a primary magnetic field of the second magnetic field is a second direction; the electric dipole is disposed in a center line of the first RF coil and an insulating layer is disposed between the electric dipole and the first RF coil; and the first direction is perpendicular to the second direction.

Abstract: Aspects of the present disclosure include a method for visualizing a fiber-like structure in a biological specimen, the method comprising: clearing the biological specimen comprising a fiber-like structure, wherein the fiber-like structure is detectably labeled; illuminating the cleared biological specimen with two light sheets from a first side and a second side to produce an image volume, wherein the second side is opposite to the first side and wherein the image volume comprises a representation of the fiber-like structure; defining a plurality of voxels within the representation of the fiber-like structure; processing each of the plurality of voxels to estimate a plurality of principal fiber-like structure orientations; and defining a starting point on the representation of the fiber-like structure and propagating a plurality of streamlines from the starting point, according to the plurality of principal fiber-like structure.

Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.

Abstract: A BCD semiconductor device includes devices integrated on a single chip. The devices include a first high voltage nLIGBT device, a second high voltage nLIGBT device, a first high voltage nLDMOS device, a second high voltage nLDMOS device, a third high voltage nLDMOS device, a first high voltage pLDMOS device and low voltage NMOS, PMOS and PNP devices, and a diode device. A dielectric isolation is applied to the high voltage nLIGBT, nLDMOS and pLDMOS devices to realize a complete isolation between the high and low voltage devices. The nLIGBT, nLDMOS, NPN and low voltage NMOS and PMOS are integrated on the substrate of a single chip. The isolation region composed of the dielectric, the second conductivity type buried layer, the dielectric trench, and the first conductivity type implanted region realizes full dielectric isolation of high and low voltage devices. The six types of high voltage transistors have multiple channels.