Abstract: The present invention relates to a method and a kit, a computer-implemented method and a system for in vitro predicting survival time of an individual with bladder cancer after surgery from an individual's biological sample. Expression levels of a target gene combination of in vitro aggressive bladder cancer specimen of a patient are detected, and the target gene combination includes at least two of PPT2, ARMH4, P4HB, SLC1A6 and ARID3A, a fragment, a homologue, a variant and a derivative thereof. Next, the expression levels are respectively compared with the reference expression levels of a reference database, and converted to a risk score sum, thereby predicting an averaged survival time of a patient having aggressive bladder cancer after surgery, and being beneficially applied to a kit and a computer-implemented method for in vitro predicting survival time of patient with most aggressive types of bladder cancer after surgery.

Abstract: In a process of molding and assembling a three-dimensional ornament, a mold having a recess in which is formed an opening is provided. A molding pattern is located inside the opening. A first material fills in the molding pattern to form a plurality of protruded decoration patterns. Then, a second material is charged in the opening and the recess of the mold over the first material to respectively form a projection and a contact wall which together form an embossment. The embossment then is removed from the mold. The embossment then is mounted inside a hollow pen tube in a manner that the projection fits in a sidewall opening of the pen tube and the protruded decoration patterns protrude from the sidewall opening. A sleeve is inserted in the hollow pen tube to urge the contact wall of the embossment against the wall of the hollow pen tube.

Abstract: In a process of molding and assembling a three-dimensional ornament, a mold having a recess in which is formed an opening is provided. A molding pattern is located inside the opening. A first material fills in the molding pattern to form a plurality of protruded decoration patterns. Then, a second material is charged in the opening and the recess of the mold over the first material to respectively form a projection and a contact wall which together form an embossment. The embossment then is removed from the mold. The embossment then is mounted inside a hollow pen tube in a manner that the projection fits in a sidewall opening of the pen tube and the protruded decoration patterns protrude from the sidewall opening. A sleeve is inserted in the hollow pen tube to urge the contact wall of the embossment against the wall of the hollow pen tube.