Abstract: Described herein are cassettes and methods for using these cassettes for cryopreserving biomaterials (e.g., a bioengineered construct or natural tissue sample) by vitrification while reducing or preventing the loss of viability associated with conventional preservation methods.

Abstract: Described herein are methods for preserving biomaterials by vitrification while reducing or preventing the loss of viability associated with conventional preservation methods. Also described herein are cassettes and methods for using these cassettes for cryopreserving biomaterials (e.g., a bioengineered construct or natural tissue sample).

Abstract: Cellular material containing living cells is preserved by combining the cellular material with a cryoprotectant formulation/medium/solution containing an effective amount of trehalose (in the absence of DMSO and/or any other added cryoprotectants) during a cryopreservation protocol. That is, the cryopreservation protocol is free of cryoprotectant other than trehalose, and the cryopreservation protocol includes: exposing the cellular material to a cryoprotectant formulation containing an effective amount of the trehalose to act as a cryoprotectant, cooling the cellular material at a cooling rate in the range of from ?3° C./minute to ?50° C./minute to a predetermined temperature below ?20° C., and obtaining a cryopreserved cellular material that has been warmed.

Abstract: Methods for ex vivo perfusion of organs (and/or tissues) with a perfusate designed to condition the organ with the desired effect being that upon transplant, said organ, having been administered said perfusate, is less likely to experience delayed graft function, deleterious effects of ischemia/reperfusion injury, including inflammatory reactions, and/or other detrimental responses that can injure the organ or recipient including precipitating or enhancing an immunological reaction from the recipient with the potential of compromising the graft's and/or recipients short teen and/or long term health and proper functionality while monitoring, sustaining and/or restoring the viability of the organ and preserving the organ for storage and/or transport.

Abstract: Large volume cellular material may be preserved by combining the cellular material with a cryoprotectant formulation/medium/solution containing at least one sugar and then subjecting the cellular material to a vitrification preservation protocol.

Abstract: Living cellular material may be preserved by incubating the cellular material in a culture medium containing at least one glycolipid, and then subjecting the cellular material to a preservation protocol.

Abstract: Large volume cellular material may be preserved by combining the cellular material with a cryoprotectant formulation/medium/solution containing at least one sugar and then subjecting the cellular material to a vitrification preservation protocol.

Abstract: Living cellular material may be preserved by incubating the cellular material in a culture medium containing at least one glycolipid, and then subjecting the cellular material to a preservation protocol.

Abstract: Methods for ex vivo perfusion of organs (and/or tissues) with a perfusate designed to condition the organ with the desired effect being that upon transplant, said organ, having been administered said perfusate, is less likely to experience delayed graft function, deleterious effects of ischemia/reperfusion injury, including inflammatory reactions, and/or other detrimental responses that can injure the organ or recipient including precipitating or enhancing an immunological reaction from the recipient with the potential of compromising the graft's and/or recipients short teen and/or long term health and proper functionality while monitoring, sustaining and/or restoring the viability of the organ and preserving the organ for storage and/or transport.

Abstract: Living cellular material may be preserved by incubating the cellular material in a culture medium containing at least one sugar, particularly for at least three hours, and then subjecting the cellular material to a preservation protocol, such as freezing, vitrification, freeze-drying and desiccation.

Abstract: Living cellular material may be preserved by incubating the cellular material in a culture medium containing at least one sugar, particularly for at least three hours, and then subjecting the cellular material to a preservation protocol, such as freezing, vitrification, freeze-drying and desiccation.

Abstract: Living cellular material may be preserved by incubating the cellular material in a culture medium containing at least one sugar, particularly for at least three hours, and then subjecting the cellular material to a preservation protocol, such as freezing, vitrification, freeze-drying and desiccation.