Abstract: The invention provides a method for detecting apoptosis in a myeloid cell comprising detecting an alteration in any one of: i) a Survivin polypeptide having an amino acid sequence as set out in SEQ ID NO: 1; ii) a polypeptide having at least 80% homology with i); iii) a nucleic acid encoding a polypeptide having the sequence set out in i) or ii); iv) a nucleic acid which hybridises under stringent conditions to the sequence set out in iii); or v) the complement of iii) or iv). The invention accordingly provides a method of modulating apoptosis in neutrophils by modulating Survivin gene expression and a method of treating inflammatory disease by modulating Survivin gene expression or function.

Abstract: The present invention relates to a method of indentifying an agent that modulates the function of an apoptosos-associated polypeptide having a sequence as set out in Table 1, the method comprising: (a) providing a sample containing an apoptosis-associated polypeptide having a sequence as set out in Table 1, and a candidate agent and incubating under conditions to permit binding of the candidate agent to the polypeptide; (b) measuring the binding of the apoptosis-associated polypeptide having a sequence as set out in Table 1 to the candidate agent in the sample; and (c) comparing the binding of the apoptosis-associated polypeptide having a sequence as set out in Table 1 to the candidate agent in the sample with the binding of the polypeptide having a sequence as set out in Table 1; wherein an increase in the binding of the apoptosis-associated polypeptide having a sequence as set out in Table 1 to the candidate agent in the sample relative to the binding of the apoptosis-associated polypeptide having a sequenc

Abstract: The invention provides a method for detecting apoptosis in a cell comprising detecting a decrease in any one of: i) an ELAVL-1 (embryonic lethal, abnormal vision, Drosophila-like 1) polypeptide having an amino acid sequences as set out in SEQ ID NO: 1; ii) a polypeptide having at least 80% homology with i); iii) a nucleic acid encoding a polypeptide having the sequence set out in i) or ii); iv) a nucleic acid which hybridises under stringent conditions to the sequence set out in iii); or v) the complement of iii) or iv). The invention accordingly provides a method of modulating apoptosis by modulating ELAVL-1 gene expression and a method for identifying genes associated with ELAVL-1 gene expression and thus identifying other genes associated with apoptosis. The invention also provides a novel nucleic acid sequence encoding the promoter region for ELAVL-1.

Abstract: The present invention relates to methods of identifying an agent that modulates the function of an apoptosis-associated polypeptide having a sequence as set out in Table 1B. The invention also relates to methods of modulating apoptosis, diagnostic methods, arrays, kits and compositions based upon the apoptosis-associated polypeptides having sequences as set out in Table 1B and in FIG. 42.

Abstract: The present invention relates to methods of identifying an agent that modulates the function of an apoptosis-associated polypeptide having a sequence as set out in Table 1B. The invention also relates to methods of modulating apoptosis, diagnostic methods, arrays, kits and compositions based upon the apoptosis-associated polypeptides having sequences as set out in Table 1B.

Abstract: The present invention relates to a method for identifying a gene product which modulates the transition of a cell between a non-apoptopic state and an apoptopic state, comprising the steps of: (a) exposing the cell to an inhibitor of GM-CSF mediated inhibition of apoptosis; and (b) exposing the cell to one or more agents which increase tyrosine phosphorylation; and (c) C) placing the cell in conditions which permit it to undergo spontaneous apoptosis; and (d) monitoring the level(s) of expression of the one or more gene products in the cell; and (e) identifying gene product(s) whose expression has been increased, decreased or modified as a result of performing steps (a) to (d).