Patents by Inventor Lianghui Ji

Lianghui Ji has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230365915
    Abstract: A culture for the fermentative production of Massoia lactone comprising an Aureobasidium melanogenum species in a culture medium, wherein the Aureobasidium melanogenum species expresses no functional Aureobasidin A synthase gene mRNA when cultured, the culture medium including KH2PO4, Na2HPO4, (NH4)2SO4, MgSO4·7H2O and CaCl2·2H2O; at least two trace elements selected from the group consisting of Fe2+, Cu2+, Zn2+ and MoO42?; urea; and a carbon source selected from the group consisting of glucose, mannose, xylose and mixtures thereof, wherein the culture medium has a pH from 5.5 to 6.5.
    Type: Application
    Filed: March 20, 2023
    Publication date: November 16, 2023
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Lianghui JI, Si Te NGOH
  • Patent number: 11639492
    Abstract: The present invention relates to the field of fermentation biotechnology, more particularly to methods for the fermentative production of massoia lactone by Aureobasidium species.
    Type: Grant
    Filed: December 12, 2019
    Date of Patent: May 2, 2023
    Assignee: Temasek Life Sciences Laboratory Limited
    Inventors: Lianghui Ji, Si Te Ngoh
  • Patent number: 11254943
    Abstract: The present invention relates to the field of molecular biology and more particularly to promoters useful for metabolic engineering in yeast or fungi for the production of biobased chemicals with broad applications. Intron-containing promoters with strong activity during oil-accumulation stages are particularly useful for genetic engineering in yeast and fungi, particularly Rhodosporidium or Rhodotorula genera. Such promoter are capable of driving strong expression of RNA or proteins in species of the Rhodosporidium or Rhodotorula genera.
    Type: Grant
    Filed: February 2, 2017
    Date of Patent: February 22, 2022
    Assignee: Temasek Life Sciences Laboratory Limited
    Inventors: Lianghui Ji, Yanbin Liu, Chong Mei John Koh, Sihui Amy Yap
  • Patent number: 11034981
    Abstract: The present invention relates to the field of fungal production fatty alcohols. More specifically, the present invention relates to genetically modified host cells, nucleic acid constructs and culture medium for the production of fatty alcohols in Rhodosporidium.
    Type: Grant
    Filed: January 29, 2018
    Date of Patent: June 15, 2021
    Assignee: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Lianghui Ji, Yanbin Liu, Chong Mei John Koh, Sihui Amy Yap, Si Te Ngoh
  • Publication number: 20200377898
    Abstract: The present invention relates to the field of molecular biology and more particularly to promoters useful for metabolic engineering in yeast or fungi for the production of biobased chemicals with broad applications. Intron-containing promoters with strong activity during oil-accumulation stages are particularly useful for genetic engineering in yeast and fungi, particularly Rhodosporidium or Rhodotorula genera. Such promoter are capable of driving strong expression of RNA or proteins in species of the Rhodosporidium or Rhodotorula genera.
    Type: Application
    Filed: February 2, 2017
    Publication date: December 3, 2020
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Lianghui JI, Yanbin LIU, Chong Mei John KOH, Sihui Amy YAP
  • Publication number: 20200157488
    Abstract: The present invention relates to the field of fermentation biotechnology, more particularly to methods for the fermentative production of massoia lactone by Aureobasidium species.
    Type: Application
    Filed: December 12, 2019
    Publication date: May 21, 2020
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Lianghui JI, Si Te NGOH
  • Patent number: 10570402
    Abstract: The present invention relates to the field of the production of transgenic plants through Agrobacterium-mediated transformation of cells of somatic embryogenic calli or embryogenic suspension cultures and regeneration of the transformed cells into fruit-setting plants. In particular, the present invention relates to the production of transgenic plants in the Euphorbiaceae family. The present invention further relates to media compositions, selection methods and engineered Agrobacterium tumefaciens strains that improve Agrobacterium-mediated transformation efficiency.
    Type: Grant
    Filed: November 14, 2018
    Date of Patent: February 25, 2020
    Assignee: Temasek Life Sciences Laboratory Limited
    Inventors: Lin Cai, Longhua Sun, Lin Fu, Lianghui Ji
  • Publication number: 20190360003
    Abstract: The present invention relates to the field of fungal production fatty alcohols. More specifically, the present invention relates to genetically modified host cells, nucleic acid constructs and culture medium for the production of fatty alcohols in Rhodosporidium.
    Type: Application
    Filed: January 29, 2018
    Publication date: November 28, 2019
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Lianghui JI, Yanbin LIU, Chong Mei John KOH, Sihui Amy YAP, Si Te NGOH
  • Patent number: 10308691
    Abstract: The present invention relates to the field of fungal biotechnology, more particularly to genetic engineering methods for the production of carotenoids in fungal hosts selected from Rhodospordium and Rhodotorula genera.
    Type: Grant
    Filed: December 14, 2015
    Date of Patent: June 4, 2019
    Assignee: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Yanbin Liu, Chong Mei Koh, Lianghui Ji
  • Publication number: 20190085345
    Abstract: The present invention relates to the field of the production of transgenic plants through Agrobacterium-mediated transformation of cells of somatic embryogenic calli or embryogenic suspension cultures and regeneration of the transformed cells into fruit-setting plants. In particular, the present invention relates to the production of transgenic plants in the Euphorbiaceae family. The present invention further relates to media compositions, selection methods and engineered Agrobacterium tumefaciens strains that improve Agrobacterium-mediated transformation efficiency.
    Type: Application
    Filed: November 14, 2018
    Publication date: March 21, 2019
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Lin CAI, Longhua SUN, Lin FU, Lianghui JI
  • Publication number: 20190010444
    Abstract: The present invention relates to the field of fermentation biotechnology, more particularly to methods for the fermentative production of massoia lactone by Aureobasidium species.
    Type: Application
    Filed: August 16, 2016
    Publication date: January 10, 2019
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Lianghui JI, Si Te NGOH
  • Patent number: 10167479
    Abstract: The present invention relates to the field of the production of transgenic plants through Agrobacterium-mediated transformation of cells of somatic embryogenic calli or embryogenic suspension cultures and regeneration of the transformed cells into fruit-setting plants. In particular, the present invention relates to the production of transgenic plants in the Euphorbiaceae family. The present invention further relates to media compositions, selection methods and engineered Agrobacterium tumefaciens strains that improve Agrobacterium-mediated transformation efficiency.
    Type: Grant
    Filed: December 4, 2009
    Date of Patent: January 1, 2019
    Assignee: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Lin Cai, Longhua Sun, Lin Fu, Lianghui Ji
  • Patent number: 10106802
    Abstract: The present invention relates to the application of isolated promoters and synthetic constructs for efficient production of genetically modified cells in a species selected from the Pucciniomycotina and Ustilaginomycotina subphyla, in particular, species selected from the Rhodosporidium, Rhodotourla, Sporobolomyces or Pseudozyma genus.
    Type: Grant
    Filed: March 10, 2014
    Date of Patent: October 23, 2018
    Assignee: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Hsin I. Cheng, Ni Peng, Lianghui Ji
  • Patent number: 10081821
    Abstract: The present invention relates to the field of fungal biotechnology, more particularly to genetic engineering methods for the production of polyunsaturated fatty acids (PUFA) in fungal hosts selected from Rhodosporidium and Rhodotorula genera. The present invention further relates to a modified fungal host cell having reduced native aldehyde dehydrogenase (ALD 1) enzyme activity, and methods for producing omega-3 and omega-6 fatty acids and triacylglycerides, by growing said fungal host cell under suitable conditions.
    Type: Grant
    Filed: August 21, 2015
    Date of Patent: September 25, 2018
    Assignee: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Yanbin Liu, Chong Mei Koh, Lianghui Ji
  • Patent number: 10081812
    Abstract: The present invention relates to the field of fungal biotechnology, more particularly to a strong inducible gene expression system in fungal species, such as a species of the Rhodosporidium genus or the Rhodotorula genus.
    Type: Grant
    Filed: March 31, 2016
    Date of Patent: September 25, 2018
    Assignee: TEMASEK LIFE SCIENCES LABORATORY LTD
    Inventors: Lianghui Ji, Yanbin Liu, Chong Mei John Koh
  • Publication number: 20180073034
    Abstract: The present invention relates to the field of fungal biotechnology, more particularly to a strong inducible gene expression system in fungal species, such as a species of the Rhodosporidium genus or the Rhodotorula genus.
    Type: Application
    Filed: March 31, 2016
    Publication date: March 15, 2018
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Lianghui JI, Yanbin LIU, Chong Mei John KOH
  • Publication number: 20170362285
    Abstract: The present invention relates to the field of fungal biotechnology, more particularly to genetic engineering methods for the production of carotenoids in fungal hosts selected from Rhodospordium and Rhodotorula genera.
    Type: Application
    Filed: December 14, 2015
    Publication date: December 21, 2017
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Yanbin LIU, Chong Mei KOH, Lianghui JI
  • Publication number: 20170198315
    Abstract: The present invention relates to the field of fungal biotechnology, more particularly to genetic engineering methods for the production of polyunsaturated fatty acids (PUFA) in fungal hosts selected from Rhodosporidium and Rhodotorula genera. The present invention further relates to a modified fungal host cell having reduced native aldehyde dehydrogenase (ALD 1) enzyme activity, and methods for producing omega-3 and omega-6 fatty acids and triacylglycerides, by growing said fungal host cell under suitable conditions.
    Type: Application
    Filed: August 21, 2015
    Publication date: July 13, 2017
    Applicant: Temasek Life Sciences Laboratory Limited
    Inventors: Yanbin LIU, Chong Mei KOH, Lianghui JI
  • Patent number: 9580363
    Abstract: The present invention relates to methods of reducing chemical fertilizer usage and greenhouse gas nitrous oxide emission and to methods of improving plant growth rate and seed productivity in agriculture through the application of a novel artificially manufactured formula containing a nitrogen-fixing bacterium that efficiently colonizes non-legume plants in aerial parts and the root system. The bacteria inocula and methods are particularly suitable for plants in the genera Jatropha, Sorghum, Gossypium, Elaeis, Ricinus, Oryza and Manihot.
    Type: Grant
    Filed: March 20, 2013
    Date of Patent: February 28, 2017
    Assignee: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Madhaiyan Munusamy, Lianghui Ji
  • Publication number: 20160032296
    Abstract: The present invention relates to the application of isolated promoters and synthetic constructs for efficient production of genetically modified cells in a species selected from the Pucciniomycotina and Ustilaginomycotina subphyla, in particular, species selected from the Rhodosporidium, Rhodotourla, Sporobolomyces or Pseudozyma genus.
    Type: Application
    Filed: March 10, 2014
    Publication date: February 4, 2016
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Hsin I. CHENG, Ni PENG, Lianghui JI