Abstract: The present invention provides a bispecific CS1-BCMA CAR-T cell and an application thereof. Specifically, the present invention provides a bispecific CAR, which comprises CS1 scFv and BCMA scFv, and a 4-1BB co-stimulatory domain and a CD3 activation domain. The bispecific CAR-T cell in the present invention has a significant killing effect on CS1 positive target cells and BCMA positive target cells, and can secrete IFN-? against target cells and significantly inhibit the growth of RPMI8226 xenograft tumor in an in vivo experiment. The present invention further provides a preparation method and an application of the bispecific CAR-T cell.

Abstract: A pulsation-free wet spraying machine resolves the issue of pulsing during pumping of a concrete spraying machine.

Abstract: A pulsation-free wet spraying machine resolves the issue of pulsing during pumping of a concrete spraying machine The wet spraying machine includes a frame, a pumping mechanism, a swing mechanism and a hydraulic system, wherein the pumping mechanism includes a hopper, a material chamber, a concrete feeding mechanism, an auxiliary feeding mechanism and a distribution valve. The hopper of the pumping mechanism is disposed above the material chamber. The distribution valve is disposed inside the material chamber, the concrete feeding mechanism and the auxiliary feeding mechanism both include a hydraulic cylinder, a concrete piston and a concrete cylinder, a discharge pipe is a three-way pipe fixed at a side surface of the material chamber, an auxiliary discharge pipe of the discharge pipe is connected with an auxiliary concrete cylinder, and a through hole is disposed on the auxiliary concrete cylinder.

Abstract: Described are a test apparatus and a test method for the wetted perimeter of coal seam water injection. In the test apparatus, a columnar insulator is provided between an upper electrode and a lower electrode, circular insulating tapes are located at the outer edges of the upper electrode and the lower electrode, a circular reverse osmosis membrane is provided at the middle of the circular insulating tape, the upper electrode, lower electrode, circular insulating tapes and circular reverse osmosis membrane form an enclosed chamber which is filled with solid sodium chloride, and cotton yarns are packed among the upper resin backing plate, lower resin backing plate, circular insulating tapes and the inner walls of water permeable perforated pipes. The upper electrode is provided with an electrode lead which passes through the columnar insulator, the lower electrode and the lower resin backing plate and goes out from the tail connecting end.

Abstract: Described are a test apparatus and a test method for the wetted perimeter of coal seam water injection. In the test apparatus, a columnar insulator is provided between an upper electrode and a lower electrode, circular insulating tapes are located at the outer edges of the upper electrode and the lower electrode, a circular reverse osmosis membrane is provided at the middle of the circular insulating tape, the upper electrode, lower electrode, circular insulating tapes and circular reverse osmosis membrane form an enclosed chamber which is filled with solid sodium chloride, and cotton yarns are packed among the upper resin backing plate, lower resin backing plate, circular insulating tapes and the inner walls of water permeable perforated pipes. The upper electrode is provided with an electrode lead which passes through the columnar insulator, the lower electrode and the lower resin backing plate and goes out from the tail connecting end.