Abstract: The present invention relates to fields of continuous directional catalysis of soybean slurry to extract protein and functional oil with nano-magnetic immobilized enzymes. The present invention discloses soybean slurry as raw material and continuous mobile phase, and nano-magnetic immobilized enzymes as stationary phase applied in three-phase magnetic fluidized bed. The present invention provides a method of enzymatic disrupting cell walls by nano-magnetic immobilized enzyme cocktail including cellulose, pectinase and alkaline protease. The method can he used to break down lipoprotein, lipopolysaccharide complex and break lipoprotein film on the surface of lipoprotein to release the lipid. The present invention also provides a method to catalyze phospholipid using nano-magnetic immobilized phospholipase to change the polarity of phospholipids and control liquid emulsification phenomenon. The enzymes disclosed in the present invention do not interfere with each other.

Abstract: The present invention provides a method for aqueous enzymatic extraction of soybean oil, which is in the field of extraction processing technology for plant oil. The method comprises treating crushed and peeled soybeans with an extrusion puffing process and a hydrolysis process with an alkaline proteinase, followed by a liquid nitrogen freezing and a high voltage electrostatic thawing process, and finally obtaining soybean oil by centrifugation. The benefits of the present invention includes short extraction time and high extraction yield. It saves up to 93.1% of time in the freezing and thawing process alone. The total oil yield is up to 95.4%. Furthermore, the method of the invention produces high quality oil with a low peroxide value, a low p-anisidine value and a low TOTOX value.