Abstract: The invention belongs to the technical field of biomedical testing, and particularly relates to a biomarker for diagnosis, treatment, and prognosis for hepatocellular carcinoma bone metastasis (HCC) and application thereof. The application of biomarker VAPA for preparing a reagent or a kit for diagnosis, treatment, and prognosis for HCC bone metastasis. The application of the reagent that detects VAPA expression levels in blood and tissues for preparing a reagent or kit for diagnosis, treatment, and prognosis for HCC bone metastasis. Compared with the diagnostic kits in the prior art that can only detect bone metastasis after it has occurred, the VAPA described in this invention offers a more specific and sensitive assay kit for the early prediction, diagnosis, disease progression assessment, treatment efficacy evaluation, drug guidance, and prognosis assessment of HCC bone metastasis. Additionally, the VAPA may also serve as a target for therapeutic interventions in bone metastasis.

Abstract: The invention belongs to the field of biotechnology and medicine, and particularly relates to the application of an inhibitor of circIKBKB and a testing reagent thereof in diagnosis, treatment and prognosis kits of breast cancer bone metastasis. The kit includes one of RT-PCR, Q-PCR, Northern blot, FISH or ISH kits. The kit includes the primers shown in SEQ ID Nos. 1-2 in the sequence listing or the probe shown in SEQ ID NO. 3 in the sequence listing for the back-splice junction sequences for circIKBKB. An antisense oligonucleotide targeting the linker sequence of circIKBKB is used as a reagent for inhibiting the generation of circIKBKB and for the preparation of a drug for the treatment of breast cancer bone metastasis. The eIF4A3-IN-2 inhibitor is used for the preparation of a treatment for breast cancer bone metastasis drug.

Abstract: The present invention relates to a non-viral iPSCs induction method as well as the compositions, kits and iPSCs obtained therefrom. More specifically, the induction method comprises the following steps: 1) Constructing a recombinant plasmid by introducing the DNA sequences expressing the reprogramming factors POU5F1, SOX2, GLIS1, KLF4, MYCL and hsa-miR-302s into an episomal vector; 2) Obtaining iPSCs by introducing the recombinant plasmids obtained in step 1) into human somatic cells, and reprogramming induction culture of the cells. The method reduces the risk of clinical applications of iPSCs by using a combination of highly-safe reprogramming factors without the introduction of high-risk reprogramming factors such as c-MYC, SV40-LT and TP53 inhibitors; The method is highly applicable.

Abstract: The present invention relates to a non-viral iPSCs induction method as well as the compositions, kits and iPSCs obtained therefrom. More specifically, the induction method comprises the following steps: 1) Constructing a recombinant plasmid by introducing the DNA sequences expressing the reprogramming factors POU5F1, SOX2, GLIS1, KLF4, MYCL and hsa-miR-302s into an episomal vector; 2) Obtaining iPSCs by introducing the recombinant plasmids obtained in step 1) into human somatic cells, and reprogramming induction culture of the cells. The method reduces the risk of clinical applications of iPSCs by using a combination of highly-safe reprogramming factors without the introduction of high-risk reprogramming factors such as c-MYC, SV40-LT and TP53 inhibitors; The method is highly applicable.

Abstract: The present invention relates to a non-viral iPSCs induction method as well as the compositions, kits and iPSCs obtained therefrom. More specifically, the induction method comprises the following steps: 1) Constructing a recombinant plasmid by introducing the DNA sequences expressing the reprogramming factors POU5F1, SOX2, GLIS1, KLF4, MYCL and hsa-miR-302s into an episomal vector; 2) Obtaining iPSCs by introducing the recombinant plasmids obtained in step 1) into human somatic cells, and reprogramming induction culture of the cells. The method reduces the risk of clinical applications of iPSCs by using a combination of highly-safe reprogramming factors without the introduction of high-risk reprogramming factors such as c-MYC, SV40-LT and TP53 inhibitors; The method is highly applicable.

Abstract: The present invention relates to a non-viral iPSCs induction composition and the kits thereof. Specifically, it comprises a recombinant plasmid, and the recombinant plasmid is obtained by constructing the DNA sequences expressing the reprogramming factors POU5F1, SOX2, GLIS1, KLF4, MYCL and hsa-miR-302s into an episomal vector; The DNA sequence of hsa-miR-302s comprises one or more sequences selected from hsa-miR-302a, hsa-miR-302b, hsa-miR-302c and hsa-miR-302d. The induction composition is suitable for a highly safe and non-integrated induction reprogramming to obtain iPSCs, which reduces the risk of the clinical applications without an introduction of the high-risk reprogramming factors such as c-MYC, SV40-LT and TP53 inhibitors.