Abstract: The present disclosure relates to use of a Clostridium ghonii spore combined with pembrolizumab in cancer treatment. It is found for the first time that the Clostridium ghonii spore combined with pembrolizumab can significantly improve a curative effect of colon cancer and reduce a dose of the pembrolizumab, and thus is efficient and low-toxic. Oncolysis by Clostridium ghonii can affect immunogenicity of a tumor microenvironment (TME) by various ways, converts an immunosuppressive state of the TME into an immune-activated state, adjusts the immunosuppressive TME, and breaks an immune tolerance. An optimal combination of the Clostridium ghonii spore and the pembrolizumab thoroughly removes about 20% of mouse tumor tissues. A benefit range of patients with tumors treated by a PD-1 antibody is expanded. The combination even has an obvious curative effect on patients failed the treatment by the PD-1 antibody.

Abstract: Methods of sequencing a protein using a novel digestion-on-emitter technology are provided.

Abstract: A roller bearing includes an outer ring, an inner ring, at least one row of rollers arranged between the outer ring and the inner ring, and at least one optical fiber cable mounted to the outer ring or the inner ring, the optical fiber cable including at least one Bragg grating. The optical fiber cable is configured such that a signal in the optical fiber cable is usable to determine a preload or load on the roller bearing.

Abstract: A selective laser sintering (SLS) device. The SLS device includes a laser forming unit, a support platform and a driving mechanism. The support platform is configured to support a plurality of raw materials for additive manufacturing of an object including a plurality of sections. The laser forming unit is disposed on the support platform and is configured to lay powders on a surface of each section of the object and sinter the powders. The driving mechanism is disposed under the laser forming unit and includes a vertical driving mechanism and a horizontal driving mechanism. The vertical driving mechanism is connected to the laser forming unit and configured to lift the laser forming unit layer by layer. The horizontal driving mechanism is configured to drive the laser forming unit to move in a horizontal direction with respect to the support platform.

Abstract: The invention belongs to the field of filament additive manufacturing, and discloses a polymer multi-material high-flexibility laser additive manufacturing system and a method thereof. The system comprises a first robot arm, a second robot arm, a positioner, a rotational extrusion nozzle in which a plurality of extrusion modules are disposed and a laser, each extrusion module is used for extruding one kind of filament, and the rotational extrusion nozzle is connected with the first robot which drives the rotational extrusion nozzle to move according to a preset trajectory; the laser is connected with the second robot, and is used for emitting a laser to fuse the filament extruded from the rotational extrusion nozzle, and through the cooperative motion of the first robot and the second robot, the extrusion and fusion of the filament are performed synchronously; the positioner serves as a forming mesa, and the rotation of the positioner cooperates with the motions of the two robots.

Abstract: A selective laser sintering (SLS) device. The SLS device includes a laser forming unit, a support platform and a driving mechanism. The support platform is configured to support a plurality of raw materials for additive manufacturing of an object including a plurality of sections. The laser forming unit is disposed on the support platform and is configured to lay powders on a surface of each section of the object and sinter the powders. The driving mechanism is disposed under the laser forming unit and includes a vertical driving mechanism and a horizontal driving mechanism. The vertical driving mechanism is connected to the laser forming unit and configured to lift the laser forming unit layer by layer. The horizontal driving mechanism is configured to drive the laser forming unit to move in a horizontal direction with respect to the support platform.

Abstract: A roller bearing includes an outer ring, an inner ring, at least one row of rollers arranged between the outer ring and the inner ring, and at least one optical fiber cable mounted to the outer ring or the inner ring, the optical fiber cable including at least one Bragg grating. The optical fiber cable is configured such that a signal in the optical fiber cable is usable to determine a preload or load on the roller bearing.

Abstract: Methods of sequencing a protein using a novel digestion-on-emitter technology are provided.

Abstract: The invention belongs to the field of filament additive manufacturing, and discloses a polymer multi-material high-flexibility laser additive manufacturing system and a method thereof. The system comprises a first robot arm, a second robot arm, a positioner, a rotational extrusion nozzle in which a plurality of extrusion modules are disposed and a laser, each extrusion module is used for extruding one kind of filament, and the rotational extrusion nozzle is connected with the first robot which drives the rotational extrusion nozzle to move according to a preset trajectory; the laser is connected with the second robot, and is used for emitting a laser to fuse the filament extruded from the rotational extrusion nozzle, and through the cooperative motion of the first robot and the second robot, the extrusion and fusion of the filament are performed synchronously; the positioner serves as a forming mesa, and the rotation of the positioner cooperates with the motions of the two robots.

Abstract: The application discloses compositions, methods, systems, and apparatuses for rapid sequence analysis of proteins, including location of post-translational modifications and disulfide bonds. Limited digestion of fully denatured antibody occurs in seconds by flowing sample in 8M urea at constant pressure through a micro column reactor containing immobilized aspergillopepsin I, resulting in a product mixture containing 3-10 kDa peptides, which is then fractionated by capillary column chromatography and analyzed by both electron transfer dissociation (ETD) and collision activated dissociation mass spectrometry. This method provides 95% sequence coverage of a mAb and detects numerous post-translational modifications. For disulfide bond location, native mAb is subjected to longer digestion times. Release of disulfide containing peptides from accessible regions of the folded protein occurs with short digestion times.

Abstract: A depolarizer, including: a photoalignment layer deposited on the substrate; and a birefringent layer deposited on the photoalignment layer; wherein the photoalignment layer includes a plurality of segments, each segment with a different fast axis orientation and a different pretilt angle, such that each segment of the birefringent layer over the corresponding segment of the photoalignment layer has a different fast axis angle and a different birefringent value.

Abstract: The application discloses, compositions, methods, systems, and apparatuses for rapid sequence analysis of proteins, including location of post-translational modifications and disulfide bonds, is described. Limited digestion of fully denatured antibody occurs in seconds by flowing sample in 8 M urea at constant pressure through a micro column reactor containing immobilized aspergillopepsin I, resulting in a product mixture containing 3-10 kDa peptides, which is then fractionated by capillary column chromatography and analyzed by both electron transfer dissociation (ETD) and collision activated dissociation mass spectrometry. This method provides 95% sequence coverage of a mAb and detects numerous post-translational modifications. For disulfide bond location, native mAb is subjected to longer digestion times. Release of disulfide containing peptides from accessible regions of the folded protein occurs with short digestion times.

Abstract: A depolarizer, including: a photoalignment layer deposited on the substrate; and a birefringent layer deposited on the photoalignment layer; wherein the photoalignment layer includes a plurality of segments, each segment with a different fast axis orientation and a different pretilt angle, such that each segment of the birefringent layer over the corresponding segment of the photoalignment layer has a different fast axis angle and a different birefringent value.