Abstract: The present invention belongs to the field of virus detection and diagnosis, and relates to a monoclonal antibody against capsid proteins of multiple AAV serotypes, a preparation method therefor, and the use thereof. Provided in the present invention are a rabbit monoclonal antibody against capsid proteins of multiple AAV serotypes, and amino acid sequences of a heavy-chain variable region and a light-chain variable region of same. Said rabbit monoclonal antibody provided by the present invention can specifically bind to capsid proteins VP1, VP2 and VP3 of multiple AAV serotypes, and thus can be used for detecting AAV antigens. The monoclonal antibody against capsid proteins of multiple AAV serotypes provided by the present invention enables and facilitates research and development processes of gene therapy vectors and detection of AAVs in basic scientific research.

Abstract: The invention belongs to the field of virus detection and diagnosis, and relates to an anti-AAV2 monoclonal antibody, and a preparation method and use thereof. Provided are an anti-AAV2 rabbit monoclonal antibody, and an amino acid sequence of a heavy chain variable region and a light chain variable region of the AAV2-resistant rabbit monoclonal antibody. The anti-AAV2 rabbit monoclonal antibody provided by the invention can be specifically combined with the VLP of the AAV2, and can be used for detecting an AAV virus antigen. The anti-AAV2 monoclonal antibody provided by the invention provides an effective detection tool for the detection of AAV2 in the research and development process of a gene therapy vector and the detection of AAV2 in basic science research.