Patents by Inventor Lidong Qin

Lidong Qin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200211496
    Abstract: The present application provides a shift register unit, a shift register circuit, a driving method, and a display apparatus, and relates to the field of display technology. The method includes: in a reset phase in which a second node is at a first level, transmitting, by a control circuit, a second level signal to a first node and an output signal terminal under the control of a voltage at the second node; and in a normal operation phase, normally operating, by the shift register unit.
    Type: Application
    Filed: November 3, 2017
    Publication date: July 2, 2020
    Inventors: Zhen Wang, Jian Sun, Fei Huang, Xiaozhou Zhan, Yun Qiao, Han Zhang, Wenwen Qin, Lele Cong, Zhengkui Wang, Rui Liu, Pengjun Chen, Lidong Wang, Shuang Zhao
  • Patent number: 10688491
    Abstract: According to the invention, generally, a method for making a microfluidic aliquoting (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. Design improvements including an injection mold design for an insert and a base and a multiplex hole punch allow for rapid fabrication of the MA chip.
    Type: Grant
    Filed: November 24, 2017
    Date of Patent: June 23, 2020
    Inventor: Lidong Qin
  • Patent number: 10500588
    Abstract: According to the invention, generally, a microfluidic aliquot (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. An insert and a base are configured to create an MA chip.
    Type: Grant
    Filed: November 24, 2017
    Date of Patent: December 10, 2019
    Inventor: Lidong Qin
  • Publication number: 20190345429
    Abstract: A device for holding a plurality of cells is provided. The device includes a top layer having a plurality of spaced apart microwells which are not in contact with each other, each microwell being open on top and sized to hold a single cell. Each microwell has a top cross sectional shape having a perimeter which includes at least one inner concave angle and at least one inner convex angle, for providing increased friction between the microwell and the cell contained within.
    Type: Application
    Filed: May 8, 2019
    Publication date: November 14, 2019
    Inventor: Lidong Qin
  • Publication number: 20190344265
    Abstract: A cell holder chip is provided, comprising a top layer having a plurality of spaced apart microwells and a plurality of microchannels, each microwell being open on top and configured to hold a single cell, and each microwell being connected to at least two nearby microwells via respective microchannels. The microchannels are sized to enable protrusions from the cells to spread therethrough and have widths smaller than the cells to preventing the cells from entering the microchannels.
    Type: Application
    Filed: May 8, 2019
    Publication date: November 14, 2019
    Inventor: Lidong Qin
  • Patent number: 10293337
    Abstract: Apparatus for isolating an individual cell from a group of cells and transferring that isolated cell to a desired location, the apparatus comprising: a single-cell pipette tip, the single-cell pipette tip comprising: a structure having a distal end; a Y-shaped microchannel formed in the structure, the Y-shaped microchannel comprising a base microchannel, a first branch microchannel and a second branch microchannel, wherein the base microchannel extends to the distal end of the structure, the first branch microchannel is connectable to a first pressure channel, the second branch microchannel is connectable to a second pressure channel, and a single-cell trap is disposed in the base microchannel, distal to the convergence of the base microchannel with the first branch microchannel and the second branch microchannel.
    Type: Grant
    Filed: January 28, 2015
    Date of Patent: May 21, 2019
    Assignee: The Methodist Hospital
    Inventors: Lidong Qin, Kai Zhang
  • Patent number: 10228369
    Abstract: Apparatus for determining the quantity of a target protein and other types of biomarkers or analytes present in a sample, the apparatus comprising: a top plate comprising a plurality of recesses arranged to form a plurality of rows extending parallel to one another; and a bottom plate comprising a plurality of recesses arranged to form a plurality of rows extending parallel to one another, and a plurality of channels extending perpendicularly to the plurality of rows of the bottom plate; wherein the top plate and the bottom plate are assembled together so that the top plate is on top of the bottom plate and the recesses of the top plate communicate with the recesses of the bottom plate so as to form a plurality of rows; and wherein at least one of the top plate and the bottom plate is configured to slide relative to the other of the top plate and the bottom plate in order to form a plurality of columns, with each of the plurality of columns in communication with each of the plurality of channels.
    Type: Grant
    Filed: August 4, 2015
    Date of Patent: March 12, 2019
    Assignee: The Methodist Hospital
    Inventors: Lidong Qin, Yujun Song
  • Publication number: 20180327706
    Abstract: The CRISPR-Cas nuclease system represents an efficient tool for genome editing and gene function analysis. It consists of two components: single-guide RNA (sgRNA) and the enzyme Cas9. The present invention introduces and optimizes a microfluidic membrane deformation method to deliver sgRNA and Cas9 into different cell types and achieve successful genome editing. This approach uses rapid cell mechanical deformation to generate transient membrane holes to enable delivery of biomaterials in the medium. The present invention has achieved high delivery efficiency of different macromolecules into different cell types, including hard-to-transfect lymphoma cells and embryonic stem cells, while maintaining high cell viability.
    Type: Application
    Filed: October 19, 2016
    Publication date: November 15, 2018
    Inventors: Lidong Qin, Xin Han, Zongbin Liu, Yuan Ma, Kai Zhang
  • Publication number: 20180156777
    Abstract: A vertical cell pairing (VCP) system comprising: a substrate having a top surface; at least one micropit formed in the top surface of the substrate, the at least one micropit being sized to seat a cell; and at least one microtrap positioned adjacent the top surface of the substrate, the at least one microtrap comprising a body having a vertical slot, wherein the vertical slot has an opening and an exit, the vertical slot being sized to pass fluid therethrough but to prevent a cell from passing therethrough; wherein the at least one microtrap is disposed relative to the at least one micropit so that a cell seated in the at least one micropit is in cell-cell communication with a cell disposed at the opening of the vertical slot.
    Type: Application
    Filed: May 2, 2016
    Publication date: June 7, 2018
    Inventors: Lidong QIN, Joon Hee JANG, Dongfang LIU
  • Publication number: 20180106797
    Abstract: Apparatus for determining the quantity of a target analyte present in a sample, the apparatus comprising: a first plate comprising a plurality of recesses arranged to form a plurality of rows extending parallel to one another, and a plurality of channels extending perpendicularly to the plurality of rows of the first plate; and a second plate comprising a plurality of recesses arranged to form a plurality of rows extending parallel to one another, and a plurality of channels extending perpendicularly to the plurality of rows of the second plate; wherein the first plate and the second plate are assembled together so that the first plate is positioned against the second plate and the recesses of the first plate communicate with the recesses of the second plate so as to form a plurality of sample rows, a plurality of control rows, and an ink row disposed between the plurality of sample rows and the plurality of control rows.
    Type: Application
    Filed: March 21, 2016
    Publication date: April 19, 2018
    Inventors: Lidong Qin, Ying Li, Ping Wang, Xifeng Wu
  • Publication number: 20180071736
    Abstract: According to the invention, generally, a microfluidic aliquot (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. An insert and a base are configured to create an MA chip.
    Type: Application
    Filed: November 24, 2017
    Publication date: March 15, 2018
    Inventor: Lidong Qin
  • Publication number: 20180071737
    Abstract: According to the invention, generally, a method for making a microfluidic aliquoting (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. Design improvements including an injection mold design for an insert and a base and a multiplex hole punch allow for rapid fabrication of the MA chip.
    Type: Application
    Filed: November 24, 2017
    Publication date: March 15, 2018
    Inventor: Lidong Qin
  • Publication number: 20180015471
    Abstract: According to the invention, generally, a microfluidic aliquoting (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. Design improvements including an injection mold design for an insert and a base and a multiplex hole punch allow for rapid fabrication of the MA chip.
    Type: Application
    Filed: September 11, 2017
    Publication date: January 18, 2018
    Inventors: Lidong Qin, Kai Zhang
  • Patent number: 9757728
    Abstract: According to the invention, generally, a microfluidic aliquoting (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by a plurality of microchannels to a plurality of side wells (outlets). A relatively large (such as 120 ?L) cell suspension having several cells may be injected into the inlet of the MA chip, and single cells may be substantially simultaneously and uniformly distributed, via positive pressure-driving flow, to the several (such as 120) side wells having single cells in less than 1 minute. The MA Chip has a high efficiency in cell recovery. Due to rapid isolation and easy identification of single cells, high cell viability, high enrichment factor, and convenient transfer of submicroliter single-cell suspension, MA Chips are well compatible with CTC isolation from blood, single-cell cloning, PCR, and sequencing.
    Type: Grant
    Filed: January 26, 2016
    Date of Patent: September 12, 2017
    Inventors: Lidong Qin, Kai Zhang
  • Publication number: 20170239657
    Abstract: Apparatus for single cell patterning, the apparatus comprising: a structure comprising a surface channel formed therein, the surface channel being connected to an inlet and an outlet; and a cell trap disposed in the surface channel, the cell trap comprising a body defining a flow diverter for diverting flow passing by the cell trap into a wide path or a narrow path, and the body and the structure together defining a well for capturing a cell diverted by the flow diverter toward the narrow path.
    Type: Application
    Filed: January 23, 2015
    Publication date: August 24, 2017
    Applicant: The Methodist Hospital
    Inventors: Lidong Qin, Kai Zhang, Ying Li
  • Publication number: 20170209863
    Abstract: According to the invention, generally, a microfluidic aliquoting (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by a plurality of microchannels to a plurality of side wells (outlets). A relatively large (such as 120 ?L) cell suspension having several cells may be injected into the inlet of the MA chip, and single cells may be substantially simultaneously and uniformly distributed, via positive pressure-driving flow, to the several (such as 120) side wells having single cells in less than 1 minute. The MA Chip has a high efficiency in cell recovery. Due to rapid isolation and easy identification of single cells, high cell viability, high enrichment factor, and convenient transfer of submicroliter single-cell suspension, MA Chips are well compatible with CTC isolation from blood, single-cell cloning, PCR, and sequencing.
    Type: Application
    Filed: January 26, 2016
    Publication date: July 27, 2017
    Inventors: Lidong Qin, Kai Zhang
  • Publication number: 20160339422
    Abstract: Apparatus for isolating an individual cell from a group of cells and transferring that isolated cell to a desired location, the apparatus comprising: a single-cell pipette tip, the single-cell pipette tip comprising: a structure having a distal end; a Y-shaped microchannel formed in the structure, the Y-shaped microchannel comprising a base microchannel, a first branch microchannel and a second branch microchannel, wherein the base microchannel extends to the distal end of the structure, the first branch microchannel is connectable to a first pressure channel, the second branch microchannel is connectable to a second pressure channel, and a single-cell trap is disposed in the base microchannel, distal to the convergence of the base microchannel with the first branch microchannel and the second branch microchannel.
    Type: Application
    Filed: January 28, 2015
    Publication date: November 24, 2016
    Inventors: Lidong Qin, Kai Zhang
  • Publication number: 20160281126
    Abstract: An improved technique for studying the molecular mechanisms of aging in eukaryotic cells utilizes an efficient, high-throughput microfluidic single-cell analysis chip in combination with high-resolution time-lapse microscopy. A High-throughput Yeast Aging and Analysis (HYAA) Chip has a plurality of discrete microfluidic channels grouped into a number of modules. Each module has a single medium inlet and a single medium outlet. Each channel in a module has a microfluidic chamber having a plurality of single-cell trapping structures, and features a sample inlet for introducing cells into the flow of medium through the chamber. This innovative design enables the determination of the yeast replicative lifespan in a high throughput manner.
    Type: Application
    Filed: June 5, 2016
    Publication date: September 29, 2016
    Applicants: Baylor College of Medicine
    Inventor: Lidong Qin
  • Publication number: 20160097767
    Abstract: Apparatus for determining the quantity of a target protein and other types of biomarkers or analytes present in a sample, the apparatus comprising: a top plate comprising a plurality of recesses arranged to form a plurality of rows extending parallel to one another; and a bottom plate comprising a plurality of recesses arranged to form a plurality of rows extending parallel to one another, and a plurality of channels extending perpendicularly to the plurality of rows of the bottom plate; wherein the top plate and the bottom plate are assembled together so that the top plate is on top of the bottom plate and the recesses of the top plate communicate with the recesses of the bottom plate so as to form a plurality of rows; and wherein at least one of the top plate and the bottom plate is configured to slide relative to the other of the top plate and the bottom plate in order to form a plurality of columns, with each of the plurality of columns in communication with each of the plurality of channels.
    Type: Application
    Filed: August 4, 2015
    Publication date: April 7, 2016
    Inventors: Lidong Qin, Yujun Song
  • Publication number: 20150233913
    Abstract: Apparatus for determining the quantity of a target protein and/or other types of biomarkers or analytes present in a sample, the apparatus comprising: a top plate comprising a plurality of recesses arranged to form a plurality of rows extending parallel to one another; and a bottom plate comprising a plurality of recesses arranged to form a plurality of rows extending parallel to one another, and a plurality of channels extending perpendicularly to the plurality of rows of the bottom plate.
    Type: Application
    Filed: October 16, 2013
    Publication date: August 20, 2015
    Inventors: Lidong Qin, Yujun Song